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Method for simultaneously and quantitatively detecting contents of melamine and cyanuric acid and application thereof

A technology of melamine and cyanuric acid, applied in measuring devices, instruments, scientific instruments and other directions, can solve the problems of increased inspection costs, great damage to instruments, cumbersome preparation process, etc., to achieve accurate and reliable test results and fast test process. , Prepare the effect of simple operation

Inactive Publication Date: 2016-01-27
CHINA INST OF VETERINARY DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since melamine and cyanuric acid coexist easily to form precipitates, if the solvent is not used properly during the simultaneous detection of the two, the two will combine to form a precipitate, and the final test result is the balance of the two in the sample after precipitation, not True content, the test results seriously deviate from the real value, making the test results unreliable
At present, the method for simultaneously determining the content of melamine and cyanuric acid is mainly liquid chromatography tandem mass spectrometry. The solvent used in this method and the chromatographic column of the mobile phase instrument are seriously damaged, which greatly shortens the service life of the chromatographic column; The equipment used in phase chromatography tandem mass spectrometry is expensive, and the detection conditions are very harmful to the instrument, which increases the cost of the test and is not conducive to the determination of large-scale samples; in addition, the sample preparation process of this method is cumbersome, and it is easy to cause The precipitation of melamine and cyanuric acid makes the detection process more difficult

Method used

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  • Method for simultaneously and quantitatively detecting contents of melamine and cyanuric acid and application thereof
  • Method for simultaneously and quantitatively detecting contents of melamine and cyanuric acid and application thereof
  • Method for simultaneously and quantitatively detecting contents of melamine and cyanuric acid and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1, quantitatively detect the content of melamine and cyanuric acid simultaneously

[0037] 1. Experimental instruments and materials

[0038] High-performance liquid chromatography: American Waters Corporation, Waters2695 type, 2489 ultraviolet detector, EmpowerⅡworkstation; Chromatographic column: HILICSilica (150×4.6mmid.5μm);

[0039] Melamine standard product: purchased from Dr. Ehrensorfer Company in Germany, with a purity of 99.0%;

[0040] Cyanuric acid standard product: purchased from Dr.Ehrensorfer Company in Germany, with a purity of 99.5%;

[0041] Acetonitrile and ammonium acetate: purchased from MERCK, USA, chromatographically pure;

[0042] Experimental water is ultrapure water;

[0043] The rest of the reagents are routine experimental reagents, analytically pure.

[0044] 2. Preparation of mobile phase, solvent and various concentrations of melamine solution and cyanuric acid solution

[0045] 1. Preparation of mobile phase

[0046] Weigh ...

Embodiment 2

[0097] Embodiment 2, the application of melamine and cyanuric acid content quantitative detection method simultaneously

[0098] One, the inventive method is used for the mensuration of melamine and cyanuric acid content in milk powder

[0099] Take 1g of milk powder (self-made milk powder from a dairy farm in Beijing, which does not contain melamine and cyanuric acid), 1g each of melamine and cyanuric acid, add solvent b to dissolve to 100mL, and ultrasonically mix to obtain the sample mother liquor; take 1mL Sample mother liquor, dilute 500 times with solvent b, filter at 0.45 μm, promptly get the test sample that melamine and cyanuric acid concentration are 20 μ g / mL, detect with HPLC (detection condition is consistent with the detection condition of three in the present embodiment) test The peak area of ​​melamine and cyanuric acid in the sample is then brought into the standard curve equation corresponding to solvent b in Table 2 to calculate the content (μg / mL) of melami...

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Abstract

The invention discloses a method for simultaneously and quantitatively detecting contents of melamine and cyanuric acid. By discoverying a sovlent capable of greatly codissolving melamine and cyanuric acid under the condtion of non-strong acid and strong base, detection results are truthful and accurate. According to the method, preparation of a sample is simple to operate, good mutual solution of melamine and cyanuric acid can be realized, and an experimental base is provided for accurate measurement of contents of melamine and cyanuric acid. An instrument used in the method is a high performance liquid chromatograph (HPLC). The equipment is cheap and easily available in comparison with equipment used in liquid chromatography tandem mass spectrometry. In addition, a solvent and a mobile phase used in the invention cause little damage to a chromatographic column of the instrument. The detection process is rapid and economic. The method is applied in simultaneously and quantitatively detecting contents of melamine and cyanuric acid, and obtained detection results are accurate and credible. The method is suitable for large-scale sample detection and is also suitable for various samples which require detection of protein content.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a simultaneous quantitative detection method for the contents of melamine and cyanuric acid and the application of the method. Background technique [0002] Melamine, commonly known as protein essence, is a triazine nitrogen-containing heterocyclic organic compound. It is a common chemical raw material and can be used to synthesize plastics, coatings, anti-folding and anti-shrinking treatment agents for textiles, heat-resistant decorative sheets, moisture-proof paper, Gray leather tanning agent, etc. Melamine is toxic to the urinary system and reproductive system of the human body (especially infants), and can even cause death. Therefore, the National Food Quality Supervision and Inspection Center clearly pointed out that melamine is a chemical raw material and is not allowed to be added to food. [0003] Protein is one of the most ingested nutrients to maintain life activities. It...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 于晓辉杨秀玉万仁玲张广川赵富华董玲玲马秋冉杨星
Owner CHINA INST OF VETERINARY DRUG CONTROL
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