Breeding method for complete set line of alfalfa three lines

Abstract

The present invention provides a breeding method for a complete set line of alfalfa three lines. The breeding method comprises the steps of: step 1, screening male sterile plants, further repeatedly evaluating the pollen fertility thereof, and marking completely male sterile and female fertile plants as MS-GN; step 2, selecting different alfalfa varieties or strains, respectively performing a test cross trial with the male sterile plants in the step 1, breeding a male parent of male sterile plants in hybrid progeny as a maintainer GN-B-1; step 3, in order to enable the sterile character of sterile lines more stable and the sterile character of maintainer GN-B-1 sterility and hybrid generation more stable, performing multi-generation backcross on maintainer materials and sterile line materials to obtain a sterile line MS-GN-2-1 of same line with the maintainer; and step 4, hybridizing the male parent in the step 2 with sterile line materials in the step 1 and recovering hybridized combination of fertility, and breeding restorer materials. The breeding method provided by the invention has the beneficial effects that the breeding method can effectively improve the output and quality of alfalfa.

Description

technical field [0001] The invention belongs to the field of crop cultivation, and in particular relates to a method for selecting a three-line matching line of alfalfa. Background technique [0002] Alfalfa is the most widely cultivated and most important perennial leguminous forage in the world. Because of its wide adaptability, high yield, high nutritional value, and good palatability, it is called the "king of forage" and is the first choice for the development of animal husbandry. As one of pastures, it is playing an increasingly important role in the development of agriculture and animal husbandry and the construction of ecological environment in China and the whole world. [0003] The alfalfa planting area in the world is more than 4 million km 2 , Breeding new varieties, improving their yield, quality, and stress resistance is an important task for forage breeders. Alfalfa is a cross-pollinated plant, which is self-aborted and pollinated mainly by insects. Alfalfa i...

AI Technical Summary

Problems solved by technology

[0003] The alfalfa planting area in the world is more than 4 million km 2 , Breeding new varieties, improving their yield, quality, and stress resistance is an important task for forage breeders. Alfalfa is a cross-pollinated plant, which is self-aborted and pollinated mainly by insects. Alfalfa is a butterfly corolla with two stamens. When the keel flap is opened, the pollen will disperse, and artificial castration and drug castration are very difficult. In view of the characteristics of flower organs and pollination characteristics of alfalfa, most breeders use single plant selection, establishment of multiple hybrid nurseries, and mixed hybrid methods to breed comprehensive varieties. With the lack of germplasm resources of alfalfa, this method leads to more and more uniform varieties, and the improvement of performance such as yield and quality is not obvious.

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