Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of blood plasma gelatin sterilized preparation and preparation method thereof

A plasma and gelatin replacement technology, applied in the field of medicine, can solve the problems of uneven product quality, insufficient sterilization, uneven heat conduction, etc., and achieve the effect of conforming to the pyrogen index, fast and balanced heat and cold conversion speed, and good effect.

Active Publication Date: 2016-11-16
WUHAN DOCAN PHARMA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional hot-press sterilization method using moist heat sterilization is 115°C for 30 minutes or 121°C for 8 minutes. Because gelatin injection is an acylated polypeptide solution with many components and a certain viscosity, it will lead to uneven heat conduction during the sterilization process. There are problems such as insufficient sterilization and uneven product quality

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of blood plasma gelatin sterilized preparation and preparation method thereof
  • A kind of blood plasma gelatin sterilized preparation and preparation method thereof
  • A kind of blood plasma gelatin sterilized preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Sol: Take 180g of commercially available medicinal bovine bone gelatin, add 2000ml of water for injection to swell at low temperature, raise the temperature to 70°C to dissolve, and dissolve for 30 minutes until the gelatin is completely dissolved.

[0041] (2) Alkaline hydrolysis: Add 1mol / L sodium hydroxide solution to adjust the pH of the gelatin solution to 9.0, raise the temperature to 95°C, maintain this temperature until the gelatin weight average molecular weight after hydrolysis is 28000-35000 Daltons, then cool down to 30 °C to obtain a degradation solution.

[0042] (3) Cross-linking and acylation: when hydrochloric acid was added to adjust the pH value of the gelatin hydrolyzate to 8.0, 6 g of cross-linking agent succinic anhydride was added for acylation, the acylation temperature was controlled at 30° C., and cross-linking was stirred for 2.0 h.

[0043] (4) Electrolyte adjustment: add potassium chloride 0.8g, sodium chloride 24g, calcium chloride 0.36...

Embodiment 2

[0047] (1) Sol: Take 190g of commercially available medicinal bovine bone gelatin, add 2000ml of water for injection to swell at low temperature, raise the temperature to 75°C to dissolve, and dissolve for 20 minutes until the gelatin is completely dissolved.

[0048](2) Alkaline hydrolysis: add 1mol / L potassium hydroxide solution to adjust the pH value of the gelatin solution to 11.0, raise the temperature to 90°C, maintain this temperature until the gelatin weight average molecular weight after hydrolysis is 28000-35000 Daltons, then cool down to 30 °C to obtain a degradation solution.

[0049] (3) Cross-linking and acylation: hydrochloric acid was added to adjust the pH value of the gelatin hydrolyzate to 9.5, 6.2 g of cross-linking agent glyoxal was added for acylation, the acylation temperature was controlled at 35° C., and cross-linking was stirred for 1.5 h.

[0050] (4) Electrolyte adjustment: Add 1.0 g of potassium chloride, 26 g of sodium chloride, and 0.40 g of calc...

Embodiment 3

[0054] (1) Sol: Take 200g of commercially available medicinal bovine bone gelatin, add 2000ml of water for injection to swell at low temperature, raise the temperature to 80°C to dissolve, and dissolve for 15 minutes until the gelatin is completely dissolved.

[0055] (2) Alkaline hydrolysis: Add 1mol / L calcium hydroxide solution to adjust the pH value of the gelatin solution to 12.0, raise the temperature to 80°C, maintain this temperature until the gelatin weight average molecular weight after hydrolysis is 28000-35000 Daltons, then cool down to 30 °C to obtain a degradation solution.

[0056] (3) Cross-linking acylation: add hydrochloric acid to adjust the pH value of the gelatin hydrolyzate to 8.5, add 6.5 g of cross-linking agent cyclohexane diisocyanate to carry out acylation, control the acylation temperature at 40°C, stir and cross-link for 1.0 h;

[0057] (4) Electrolyte adjustment: add potassium chloride 1.2g, sodium chloride 28g, calcium chloride 0.44g, add appropri...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
distribution coefficientaaaaaaaaaa
distribution coefficientaaaaaaaaaa
Login to View More

Abstract

The invention relates to a preparation method of a dextran-gelatin sterilization preparation. The preparation method includes (1) gelatin dissolving, (2) basic hydrolysis, (3) crosslinking acylation, (4) electrolyte regulation, (5) adsorption and clarification and (6) rotating sterilization. The invention further relates to the dextran-gelatin sterilization preparation prepared by the preparation method. The weight-average molecular weight of the dextran-gelatin sterilization preparation ranges from 28000 to 35000, the weight-average molecular weight of 10% of macromolecules is 90000 or below, the weight-average molecular weight of 10% of macromolecules is 8000 or above, and a distribution coefficient Mw / Mn is 2.5 or below.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of a plasma substitute gelatin preparation, in particular to a method for preparing a plasma substitute gelatin sterilized preparation using bovine bone gelatin as a raw material, and the plasma substitute gelatin sterilized preparation prepared by the method. Background technique [0002] Substitute plasma gelatin is a new generation of improved gelatin, which is a commonly used drug in the medical field. Its injection is a gelatin-based plasma substitute recommended by the World Health Organization's "Guidelines for Essential Medicines", and is widely used clinically for hemorrhagic shock and fluid loss shock caused by various reasons. It has the function of rapidly replenishing blood volume and maintaining blood pressure. It is suitable for clinical first aid, does not need blood type matching during blood transfusion, can avoid cross infection, and has ad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/17A61K35/32A61K9/08C08H1/00C08J3/24A61P7/08
Inventor 黄岭尹磊张伊萌胡淑静张文军
Owner WUHAN DOCAN PHARMA