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A kind of bacillus subtilis and microbial agent and their application in fermentation and decomposing

A technology of Bacillus subtilis and microbial inoculum, which is applied in the field of agricultural biology, can solve the problem that the efficiency of fermentation and decomposing needs to be improved, and achieve the effects of improving the efficiency of fermentation and decomposing and improving the efficiency.

Active Publication Date: 2018-08-17
BEIJING HUIMINDA TECH DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation and decomposing efficiency of the existing microbial strains still needs to be improved, especially the fermentation and decomposing efficiency of landscaping waste piles needs to be improved urgently.

Method used

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  • A kind of bacillus subtilis and microbial agent and their application in fermentation and decomposing
  • A kind of bacillus subtilis and microbial agent and their application in fermentation and decomposing
  • A kind of bacillus subtilis and microbial agent and their application in fermentation and decomposing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] This example is used to illustrate the cultivation of Bacillus subtilis and the preparation of microbial inoculum of the present invention.

[0048] The slant strain of Bacillus subtilis (Bacillus subtilis) with the preservation number of CCTCC NO: M2013496 was placed on an LB plate (containing 10g / L of peptone, 5g / L of yeast extract, 5g / L of NaCl and 3g / L of agar sugar, pH value 7.4, the same below), and cultured at 37°C for 15 hours, picked a single colony from the plate, and inoculated it in 25mL of LB liquid medium (containing 10g / L peptone, 5g / L Yeast extract and 5g / L NaCl, pH value 7.4, hereinafter the same), cultivated on a shaker at 250r / min at 37°C for 15 hours to obtain primary seeds, and added 20mL of primary seeds to 500mL of LB liquid medium In 250r / min shaker at 37°C for 15 hours to obtain secondary seeds, 2L of secondary seeds were added to 50L of LB liquid medium, stirred at 250r / min at 37°C and 10m 3 / h ventilation for 15 hours to obtain a culture solu...

Embodiment 2

[0082] This example is used to illustrate the cultivation of Bacillus subtilis and Bacillus licheniformis of the present invention and the preparation of microbial inoculum.

[0083] The slant strain of Bacillus subtilis (Bacillus subtilis) with the preservation number of CCTCC NO: M2013496 was placed on an LB plate (containing 10g / L of peptone, 5g / L of yeast extract, 5g / L of NaCl and 3g / L of agar sugar, pH value 7.4, the same below), and cultured at 37°C for 15 hours, picked a single colony from the plate, and inoculated it in 25mL of LB liquid medium (containing 10g / L peptone, 5g / L Yeast extract and 5g / L NaCl, pH value 7.4, hereinafter the same), cultivated on a shaker at 250r / min at 37°C for 15 hours to obtain primary seeds, and added 20mL of primary seeds to 500mL of LB liquid medium , cultivated on a shaker at 250 r / min at 37°C for 15 hours to obtain secondary seeds of Bacillus subtilis.

[0084] The slant strain of Bacillus licheniformis (Bacillus licheniformis) with th...

Embodiment 3

[0088] The microbial inoculum was prepared according to the method of Example 2, except that 1.2 L of secondary seeds of Bacillus subtilis and 1.2 L of secondary seeds of Bacillus licheniformis were mixed, and then tertiary expansion culture was carried out.

[0089] In this embodiment, the CFU ratio of the Bacillus subtilis to the Bacillus licheniformis in the culture medium was determined to be 1:0.9 by using the nucleic acid quantification method based on 16S rDNA.

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Abstract

The invention provides bacillus subtilis, a microbial agent and application of the bacillus subtilis or the microbial agent to fermentation compost maturity. A preservation number of the bacillus subtilis is CCTCC NO. M2013496. The microbial agent contains the bacillus subtilis which is used as an effective ingredient. The bacillus subtilis, the microbial agent and the application have the advantages that the bacillus subtilis or the microbial agent can be used for fermentation compost maturity of crop straw and garden greening waste, and the fermentation compost maturity promotion level can be greatly enhanced.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a bacillus subtilis, a microbial agent and their application in fermentation and decomposing. Background technique [0002] Straw mainly refers to cotton stalks, and also refers to the stalks of other crops. It usually refers to the remaining parts of wheat, rice, corn, potatoes, oilseeds, sugar cane and other crops after harvesting seeds. Straw contains a large amount of organic matter, nitrogen, phosphorus, potassium and trace elements, and is one of the important organic fertilizer sources for agricultural production. According to measurements, the stalks, leaves, and roots of straw contain a large amount of nitrogen, phosphorus, and potassium organic raw materials. According to the analysis, every 100 kg of fresh straw contains 0.48 kg of nitrogen, 0.38 kg of phosphorus, and 1.67 kg of potassium, which is equivalent to 2.4 kg of nitrogen fertilizer. , 3.8 kg of phos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/125C12R1/10
CPCY02W30/40
Inventor 郝元福
Owner BEIJING HUIMINDA TECH DEV CENT