Sheep blood IgG extract product and application thereof

A technology of extract and blood, applied in the field of IgG extract, can solve the problems of long desalination process, low purification factor, poor resolution, etc., and achieve the effect of wide application and promotion prospects, simple preparation and operation, and low cost

Inactive Publication Date: 2016-02-24
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two technical solutions are faced with the following outstanding problems: firstly, the resolution is poor, the purification factor is low, and non-immunoglobulin components such as albumin and fibrin are easily introduced into the product; secondly, the desalination process takes a long time and consumes a lot of water

Method used

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  • Sheep blood IgG extract product and application thereof
  • Sheep blood IgG extract product and application thereof
  • Sheep blood IgG extract product and application thereof

Examples

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Embodiment Construction

[0027] In the following embodiments, the applicant provides several specific implementations of the present invention, and the raw materials, equipment, preparations, etc. used below are only illustrative and do not constitute special limitations. Improvements, substitutions, and adjustments made by those skilled in the art on the basis of understanding the essence and spirit of the present invention still fall within the protection scope of the present invention.

[0028] In the following experiments, the sheep whole blood used was anticoagulated with 10% potassium oxalate, and the anticoagulated blood was centrifuged at 5000rpm×15min, and the red blood cells were discarded to prepare plasma, which was stored at 4°C for future use.

[0029] In the following experiments, the various reagents and experimental equipment used can be purchased from the market, and can also be prepared according to needs.

[0030] During the extraction of immunoglobulin extracts, the steps of salti...

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Abstract

The invention discloses a sheep blood IgG extract product. The extract product is prepared by the following steps: 1) adding ammonium sulfate in the sheep blood plasma until 20% of a saturation degree is reached, standing the material and centrifuging the material to obtain a supernatant, adding ammonium sulfate in the supernatant until 50% of the saturation degree is reached, standing a mixture and centrifuging the mixture to obtain precipitate, dissolving the precipitate and adding the ammonium sulfate until 33% of the saturation is reached, and standing the precipitate and centrifuging the precipitate to obtain the precipitate which is an IgG extract product crude product; and 2) using 100kd ultrafilter membrane for performing ultrafiltration on the IgG extract product crude product for at least one time to realize desalination and condensation, drying an interception object of the ultrafilter membrane to obtain the IgG extract product. The sheep blood IgG extract product has high immunocompetence, can provide passive immune protection for newborn lambs, and can obtain better production benefit.

Description

technical field [0001] The invention relates to an animal feed additive, in particular to an IgG extract made from sheep blood and its application. Background technique [0002] In modern animal husbandry, in order to improve the survival rate of newborn animals and reduce their disease, antibiotics, probiotics and other biological agents are usually added to the feed. Continuous research over the years has shown that large-scale and widespread use of antibiotics can lead to gastrointestinal bacteria The emergence of drug resistance makes the amount of antibiotics used more and more, and the cost of application is higher and higher, and its residues in animal products also directly threaten human health and safety; while the use of probiotics is less effective, and the use of prebiotics The survival time of probiotics in the intestinal tract and digestive tract of animals is short, and the reproduction amount is low, which makes it difficult for them to produce ideal effects...

Claims

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Application Information

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IPC IPC(8): C07K16/06C07K1/36C07K1/34C07K1/30A23K20/147A23K50/00
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