Freeze-drying protective agent applied to nucleic acid amplification system

Abstract

The invention discloses a freeze-drying protective agent applied to a nucleic acid amplification system. The freeze-drying protective agent is prepared from one or more of trehalose and water treated through diethylpyrocarbonate. The freeze-drying protective agent applied to the nucleic acid amplification system further comprises cane sugar and / or lactose. A preparing method of the freeze-drying protective agent applied to the nucleic acid amplification system includes the following steps that the components are mixed accordingly the use concentration ratio to be even, and the freeze-drying protective agent is obtained. By means of the freeze-drying protective agent, the fluorescence quantitative nucleic acid diagnostic reagent system of RNA / DNA can be detected in a freeze-drying mode, freeze drying can be achieved, the detecting aim can be achieved only through one-step hydration, and the normal-temperature transportation aim and the normal-temperature storage aim which can not be achieved through a nucleic acid diagnostic reagent at the present stage can be achieved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a freeze-drying protective agent applied to a nucleic acid amplification system. Background technique [0002] Polymerase chain reaction (PCR) technology has been used in medical testing and clinical testing since 1989 based on its advantages of rapidity, simplicity, sensitivity, and specificity, and soon became one of the main detection methods in medicine and diagnostics. [0003] DNA nucleic acid amplification is a molecular biology technique used to amplify specific DNA fragments. Using DNA denaturation at high temperature in vitro will become a single strand. At low temperature, primers and single strands will combine according to the principle of base complementary pairing, and then adjust the temperature to the optimum reaction temperature of DNA polymerase. DNA polymerase will follow the phosphoric acid to the five-carbon sugar Complementary strands are synthes...

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