Freeze-drying protective agent applied to nucleic acid amplification system

Abstract

The invention discloses a freeze-drying protective agent applied to a nucleic acid amplification system. The freeze-drying protective agent is prepared from one or more of trehalose and water treated through diethylpyrocarbonate. The freeze-drying protective agent applied to the nucleic acid amplification system further comprises cane sugar and / or lactose. A preparing method of the freeze-drying protective agent applied to the nucleic acid amplification system includes the following steps that the components are mixed accordingly the use concentration ratio to be even, and the freeze-drying protective agent is obtained. By means of the freeze-drying protective agent, the fluorescence quantitative nucleic acid diagnostic reagent system of RNA / DNA can be detected in a freeze-drying mode, freeze drying can be achieved, the detecting aim can be achieved only through one-step hydration, and the normal-temperature transportation aim and the normal-temperature storage aim which can not be achieved through a nucleic acid diagnostic reagent at the present stage can be achieved.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a freeze-drying protective agent applied to a nucleic acid amplification system. Background technique [0002] Polymerase chain reaction (PCR) technology has been used in medical testing and clinical testing since 1989 based on its advantages of rapidity, simplicity, sensitivity, and specificity, and soon became one of the main detection methods in medicine and diagnostics. [0003] DNA nucleic acid amplification is a molecular biology technique used to amplify specific DNA fragments. Using DNA denaturation at high temperature in vitro will become a single strand. At low temperature, primers and single strands will combine according to the principle of base complementary pairing, and then adjust the temperature to the optimum reaction temperature of DNA polymerase. DNA polymerase will follow the phosphoric acid to the five-carbon sugar Complementary strands are synthes...

AI Technical Summary

Problems solved by technology

[0006] Nucleic acid diagnosis is widely used in the detection of various pathogens, but nucleic acid amplification reaction reagents are unstable when stored at room temperature, and become invalid in a few weeks; when stored at 2-8°C, the storage period is only about three months; Although the storage period at -20°C can be as long as one year, it must be repeatedly frozen and thawed each time it is used, which also has a great impact on the activity of the reagent, and the components cannot be mixed together. Trouble and other disadvantages, the long-term storage and long-distance transportation of the kit will be greatly restricted, and the sensitivity of the diagnostic reagent will be reduced or even completely invalid due to improper storage temperature, which will eventually lead to the untimely detection of the disease and cause the epidemic

[0007] At present, nucleic acid amplification reaction reagents on the market lack methods that can be stored at 4°C or room temperature for a long time, which also limits the market for nucleic acid diagnostic reagent products

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