Method for increasing weight of Bombyx mori pupa bodies

A technology of body weight and silkworm, applied in the directions of botanical equipment and methods, biochemical equipment and methods, recombinant DNA technology, etc., can solve problems such as lack of research, and achieve the effect of improving the physiological function of silkworms

Inactive Publication Date: 2016-02-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effect of silkworm Corazonin is mediated through the silkworm Corazonin receptor (BmCrzR), but there is a lack of research on how BmCrzR mediates the effect of Corazonin to regulate the growth of silkworm pupae and material and energy metabolism.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Total RNA was extracted from silk glands of silkworms on the fourth day of the fifth instar, and then reverse-transcribed to obtain the first-strand cDNA: dissect and collect the silk glands of silkworms on the fourth day of the fifth instar, use Trizol kit to extract total RNA, and then use reverse transcription to obtain the first strand cDNA The 1-strand cDNA synthesis reagent was used to reverse-transcribe the total RNA; the reverse transcription conditions were: 65°C denaturation for 5 minutes, 42°C reaction for 60 minutes, and 70°C inactivation for 15 minutes to obtain the first-strand cDNA.

[0030] Using the first-strand cDNA as a template, the forward primer 5'-AAGCTTGCCACCATGGACAACGAAGGCAACAGTAC-3' and the reverse primer 5'-GGATCCCGTAACAAACTAATGTTCTGTCCATTCG-3' were used for reverse transcription polymerase chain reaction (RT-PCR) amplification, and the clone was obtained Bombyx mori Corazonin receptor (BmCrzR) cDNA. The RT-PCR amplification conditions were: p...

Embodiment 2

[0036] Total RNA was extracted from silk glands of silkworms on the third day of the fifth instar, and then reverse-transcribed to obtain the first-strand cDNA: dissect and collect the silk glands of silkworms on the third day of the fifth instar, use Trizol kit to extract total RNA, and then use reverse transcription to obtain the first strand cDNA The 1-strand cDNA synthesis reagent was used to reverse-transcribe the total RNA; the reverse transcription conditions were: 65°C denaturation for 5 minutes, 42°C reaction for 60 minutes, and 70°C inactivation for 15 minutes to obtain the first-strand cDNA.

[0037] Using the first-strand cDNA as a template, the forward primer 5'-AAGCTTGCCACCATGGACAACGAAGGCAACAGTAC-3' and the reverse primer 5'-GGATCCCGTAACAAACTAATGTTCTGTCCATTCG-3' were used for reverse transcription polymerase chain reaction (RT-PCR) amplification, and the clone was obtained Bombyx mori Corazonin receptor (BmCrzR) cDNA. The RT-PCR amplification conditions were: pre...

Embodiment 3

[0043] Total RNA was extracted from silk glands of silkworms on the fourth day of the fifth instar, and then reverse-transcribed to obtain the first-strand cDNA: dissect and collect the silk glands of silkworms on the fourth day of the fifth instar, use Trizol kit to extract total RNA, and then use reverse transcription to obtain the first strand cDNA The 1-strand cDNA synthesis reagent was used to reverse-transcribe the total RNA; the reverse transcription conditions were: 65°C denaturation for 5 minutes, 42°C reaction for 60 minutes, and 70°C inactivation for 15 minutes to obtain the first-strand cDNA.

[0044] Using the first-strand cDNA as a template, the forward primer 5'-AAGCTTGCCACCATGGACAACGAAGGCAACAGTAC-3' and the reverse primer 5'-GGATCCCGTAACAAACTAATGTTCTGTCCATTCG-3' were used for reverse transcription polymerase chain reaction (RT-PCR) amplification, and the clone was obtained Bombyx mori Corazonin receptor (BmCrzR) cDNA. The RT-PCR amplification conditions were: p...

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PUM

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Abstract

The invention discloses a method for increasing weight of Bombyx mori pupa bodies. The method includes the following steps: extracting total RNA from fifth instar Bombyx mori silk glands; performing reverse transcription to obtain first-chain cDNA, and performing Rt-PCR amplification by taking the first-chain cDNA as a template; obtaining BmCrzR cDNA through cloning; performing PCR amplification by taking BmCrzR cDNA as a template; after enzyme digestion, connecting DNA segments with a carrier to obtain pFLAG-BmCrzR; performing PCR amplification by taking pFLAG-BmCrzR as a template to obtain a reaction template; mixing the reaction template with T7 reaction buffer, ATP liquid, CTP liquid, GTP liquid, UTP liquid and T7 enzyme for reaction, and obtaining BmCrzR dsRNA after further treatment; finally, injecting BmCrzR dsRNA into abdomens of larvae of fifth instar Bombyx mori; feeding with folium mori until Bombyx mori are mature and cocooning is formed and weight of Bombyx mori pupa bodies is increased. The method for increasing weight of Bombyx mori pupa bodies is realized through controlling Bombyx mori metabolism rather than increasing eating quantity of folium mori, so that the method has positive functions on studying the substance and energy metabolism mechanism of Bombyx mori and further improving the physiological function of Bombyx mori.

Description

technical field [0001] The invention relates to a method for increasing the weight of silkworms, in particular to a method for increasing the weight of silkworm chrysalis. Background technique [0002] The silkworm (Bombyxmori) is the largest economic resource insect reared indoors on the earth today. It absorbs various nutrients from mulberry leaves (feed), and supplies various tissues and organs in the silkworm through metabolic and physiological processes for growth, development, and reproduction. . Silkworm large-scale rearing is divided into cocoon production and silkworm egg production. The main purpose of silkworm egg production is to obtain high-quality and high-yield silkworm eggs, which are closely related to the regulation of silkworm pupal growth and metabolic physiological processes. [0003] Neuropeptides play an important role in the regulation of important physiological processes such as material and energy metabolism in insects. There are nearly 40 kinds o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/85A01K67/04
Inventor 时连根杨静文
Owner ZHEJIANG UNIV
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