A portable rapid test strip for syphilis for household use

Abstract

A portable rapid test strip for syphilis for household use is disclosed. Syphilis antigens TP17 and TP47 are adopted to construct an escherichia coli expression vector. Through codon optimization, carrying of purification tags, construction of the syphilis antigen expression vector and addition of a molecular chaperone signal peptide having a molecular weight of 12 KD, a target gene can be expressed efficiently in vitro and the exogenous target gene in a form of a soluble protein is secreted into periplasm, and therefore subsequent purification of target antigens is convenient. Through codon preference of the escherichia coli, the two antigen genes of the syphilis are optimized and artificially synthesized, efficient expression of the syphilis antigens in the escherichia coli can be achieved, and soluble expression can be achieved through controlling expression conditions, thus avoiding tedious and inefficient denaturation and renaturation processes of inclusion bodies. In terms of clone strategies, the C ends of the antigens carry the purification tags, and therefore product purification steps are simplified. The test strip is rapid, simple, economical, easily popularized, easily available in materials, and not restricted by experiment conditions.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a portable family-type rapid detection test strip for syphilis. Background technique [0002] At present, the cultivation of syphilis pathogen in vitro is still not realized. Therefore, the clinical and laboratory diagnosis of syphilis mainly focuses on the detection of serological features. Although the diagnosis can also be made by directly identifying the pathogen, the existing technology mainly uses a dark field microscope to directly observe the sample. Such an operation is highly subjective, and false negatives may occur even for very experienced testers. Results; PCR method can also directly detect the presence of pathogens, but this method is expensive and not easy to be used as a routine test in blood stations and hospitals. It can be seen that serological detection of syphilis patients is very important in the diagnosis of syphilis. Serological diagnosis ...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a portable home-type rapid detection test strip for syphilis, which aims to solve the problems that the traditional syphilis diagnosis method is cumbersome, time-consuming, expensive and difficult to popularize

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