A marker related to auxiliary diagnosis of non-small cell lung cancer and its application
A non-small cell lung cancer, diagnostic marker technology, applied in the biological field, can solve the problems of unclear role of human tumors, no human cancer detection and target therapy, and low early diagnosis rate.
Active Publication Date: 2018-12-25
CHENGDU SHANQUANJIANG BIOTECH CO LTD
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Problems solved by technology
An important reason is that the early diagnosis rate is extremely low, less than 2%, and more than 80% of patients are already in the advanced stage when they see a doctor.
Previous studies on NATD have focused on yeast 16 , but its role in the development of human tumors remains unclear
So far, there have been no reports about using NATD as a marker for human cancer detection and target therapy
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[0046] The present invention uses Real-time PCR and immunohistochemical technology to analyze the expression level of NATD in the tumor specimens and normal tissues of 29 cases of non-small cell lung cancer patients, and finds that the expression level of NATD in the tumor tissues of lung cancer patients is significantly increased, and positively correlated with patient mortality. A stable non-small cell lung cancer cell line with NATD knockdown was constructed, and the migration ability of cancer cells was detected to be impaired. Immunohistochemistry and Real-time PCR detection showed that NATD can regulate the expression of key molecular genes in the process of EMT, and the decrease of NATD expression level can inhibit the conversion process of EMT. NATD without catalytic activity (abbreviated as NATDΔ) was constructed using mutation technology, and in vitro acetylation experiments verified the necessity of the missing acetyl-CoA binding site for the biological activity of ...
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The invention relates to the field of molecular biology and tumor pharmacology and discloses a marker related to auxiliary diagnosis of cancer cells or tumors. The marker is NATD or a combination of NATD and Slug. The invention provides a quick, simple and convenient detection method of the non-small cell lung cancer marker. The detection method can serve as an auxiliary means for early diagnosis of the non-small cell lung cancer or other urothelial tumors. In addition, NATD serving as an epigenetic inheritance modification enzyme can screen antibodies or small molecule compounds specific to protein to inhibit activity of the protein, so that the objective of treating the non-small cell lung cancer and other urothelial tumors is achieved.
Description
[0001] Patent Description [0002] All documents mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. In addition, it should be understood that after reading the teaching content of the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application. field of invention [0003] The invention belongs to the field of biotechnology and provides a new method for detection, diagnosis, prognosis and treatment of non-small cell lung cancer. Specifically, the present invention relates to N-alpha-acetyltransferase D (NATD) used alone or in combination with Slug protein as a specific diagnostic marker for non-small cell lung cancer, for the prognosis of patients with non-small cell lung cancer, and with Application as a potential target for the tr...
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IPC IPC(8): C12N15/54C12N15/12C12N15/11C12N15/113C12N9/10C07K14/47C07K16/40C12Q1/6886G01N33/68
CPCC07K14/47C07K16/40C12N9/1029C12Q1/6886C12Q2600/158G01N33/68
Inventor 赵权魏继武曾长江鞠君毅陈爱萍
Owner CHENGDU SHANQUANJIANG BIOTECH CO LTD