Preparation method of reagent plate for detecting sterigmatocystin

Abstract

The invention discloses a preparation method of a reagent plate for detecting sterigmatocystin. A colloidal gold binding pad of a nitrocellulose film is coated with an anti-sterigmatocystin monoclonal antibody-colloidal gold mark, a detection line and a quality control line are orderly arranged from a sample pad to a water absorption pad and are respectively coated with a sterigmatocystin-carrier protein conjugate and goat anti-mouse IgG, and the carrier protein is a rabbit serum protein. The nitrocellulose film is spray-coated with the sterigmatocystin-carrier protein conjugate and goat anti-mouse IgG through a film formation machine so that the detection line and the quality control line are obtained, the nitrocellulose film is dried by an oven at a temperature of 38 DEG C for 8h, the colloidal gold binding pad is spray-coated with the anti-sterigmatocystin monoclonal antibody-colloidal gold mark through the film formation machine, and the colloidal gold binding pad is dried by the oven at a temperature of 38 DEG C for 8h. The reagent plate has the advantages of fast detection rate, sensitivity, accuracy, quantification and simple operation. The preparation method has simple processes, realizes detection of a single sample, has a low production cost and greatly reduces a detection cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing a reagent plate for detecting aspergillus versicolor. Background technique [0002] Sterigmatocystin is mainly the final metabolite of Aspergillus versicolor and Aspergillus nidulans, and it is also the late stage of the synthesis of aflatoxins by Aspergillus flavus and Aspergillus parasiticus Speare mid product. It is reported that corn infected with Aspergillus versicolor can produce more than 12g / kg of Aspergillus versicolor in 21 days under the environment of 27°C. [0003] The oral LD50 of Aspergillus versicolor in rats is measured in mg / kg body weight, 166 for males, 120 for females, and more than 800 for mice. Monkey intraperitoneally injected LDso was 32. It can be seen from the LDso value that primates (monkeys) are more sensitive to Aspergillus versicolor than rodents. The fatal lesions caused by Aspergillus versicolor were mainly liver and kidney...

AI Technical Summary

Problems solved by technology

At present, there is no rapid and accurate kit to detect it