Pteris wallichiana tissue culture rapid breeding method
A technology of tissue culture and Pteris fern, applied in gardening methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of unqualified breeding technology, destruction of wild fern resources, long time required, etc., and achieve reduction Effects of cultivation steps, shortening of cultivation time, and reduction of cultivation cost
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[0018] Example 1
[0019] An example of the rapid propagation method of Pteridium aquilinum tissue culture according to the present invention includes the following steps:
[0020] (1) Selection and disinfection of explants: Take the mature spores of Pteris sylvestris as explants, slowly rinse the explants under running water to remove the dirt on the surface, put them in a beaker, and use 2v / v% first After soaking in the cleansing liquid for 15 minutes, rinse with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then use 1-2 drops of Tween-20 100ml concentration is 0.1v / v%HgC1 2 Sterilize for 8-15 minutes, soak in sterile water for 3 times, use sterile filter paper to absorb moisture on the surface of the explants and then inoculate, where the sterile water is distilled water sterilized by high temperature and high pressure;
[0021] (2) Induction of spores: inoculate the explants obtained in step (...
Example Embodiment
[0025] Example 2
[0026] An example of the rapid propagation method of Pteridium aquilinum tissue culture according to the present invention includes the following steps:
[0027] (1) Selection and disinfection of explants: Take the mature spores of Pteris sylvestris as explants, slowly rinse the explants under running water to remove the dirt on the surface, put them in a beaker, and use 2v / v% first After soaking in the cleansing liquid for 15 minutes, rinse with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then use 1-2 drops of Tween-20 100ml concentration is 0.1v / v%HgC1 2 Sterilize for 8-15 minutes, soak in sterile water for 3 times, use sterile filter paper to absorb moisture on the surface of the explants and then inoculate, where the sterile water is distilled water sterilized by high temperature and high pressure;
[0028] (2) Induction of spores: inoculate the explants obtained in step (...
Example Embodiment
[0032] Example 3
[0033] An example of the rapid propagation method of Pteridium aquilinum tissue culture according to the present invention includes the following steps:
[0034] (1) Selection and disinfection of explants: Take the mature spores of Pteris sylvestris as explants, slowly rinse the explants under running water to remove the dirt on the surface, put them in a beaker, and use 2v / v% first After soaking in the cleansing liquid for 15 minutes, rinse with running water for 10 minutes, move the explants to the ultra-clean workbench, disinfect the surface with 75v / v% alcohol for 15s, and then use 1-2 drops of Tween-20 100ml concentration is 0.1v / v%HgC1 2 Sterilize for 8-15 minutes, soak in sterile water for 3 times, use sterile filter paper to absorb moisture on the surface of the explants and then inoculate, where the sterile water is distilled water sterilized by high temperature and high pressure;
[0035] (2) Induction of spores: inoculate the explants obtained in step (...
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