Chiral polyoxometalate and its preparation method and application
An oxoacid and chiral technology, which is applied in the field of chiral polyoxometalate and its preparation, can solve the problems of lack of targeting, clinical application and poor recognition ability of POMs, and achieve the elimination of cytotoxicity, Good suppression effect and high sensitivity effect
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[0039] The preparation method of chiral polyoxometalate of the present invention comprises the following steps:
[0040] (1) Dissolve the aminated Anderson-type POM and succinic anhydride in anhydrous acetonitrile according to the amount of substance 1: (2-10), stir and react at room temperature for more than 24h, generally stir at 200-400r / min for 24-48h, remove Precipitate, and the filtrate is placed in ether atmosphere for crystallization to obtain crystals, which are carboxylated Anderson-type POM;
[0041] (2) Dissolve carboxylated Anderson-type POM, NHS and DCC in anhydrous DMF according to the amount of substances 1:1:1, stir at room temperature for more than 24h, generally stir at 200-400r / min for 24-48h, and remove the precipitate. The filtrate was crystallized in an ether atmosphere to obtain crystals, which were carboxyl-activated Anderson-type POM;
[0042] (3) Dissolve carboxyl-activated Anderson-type POM and chiral amino acid in anhydrous DMF according to the ra...
Embodiment 1
[0052] Chiral polyoxometalate, structural formula as shown in formula (I), in formula (I), R is
[0053]
[0054] The preparation method of above-mentioned inhibitor, comprises the following steps:
[0055] (1) Dissolve aminated Anderson-type POM and succinic anhydride in anhydrous acetonitrile according to the amount of substances 1:2, stir and react at room temperature for 24 hours, remove the precipitate, and crystallize the filtrate in an ether atmosphere to obtain crystals, which are carboxyl groups Anderson type POM;
[0056] (2) Carboxylated Anderson-type POM, NHS and DCC were dissolved in anhydrous DMF according to the amount of substances 1:1:1, stirred and reacted at room temperature for 24 hours, and the precipitate was removed. The filtrate was crystallized in an ether atmosphere to obtain crystals, namely Activate Anderson-type POM for carboxyl groups;
[0057] (3) Carboxyl-activated Anderson-type POM and L-His were dissolved in anhydrous DMF at a ratio of 1:...
Embodiment 2
[0059] Chiral polyoxometalate, structural formula is shown in formula (I), and R is
[0060]
[0061] The preparation method of above-mentioned inhibitor, comprises the following steps:
[0062] (1) Dissolve the aminated Anderson-type POM and succinic anhydride in anhydrous acetonitrile according to the amount of substances 1:4, stir and react at room temperature for 24 hours, remove the precipitate, and crystallize the filtrate in an ether atmosphere to obtain crystals, which are carboxyl groups Anderson type POM;
[0063] (2) Carboxylated Anderson-type POM, NHS and DCC were dissolved in anhydrous DMF according to the amount of substances 1:1:1, stirred and reacted at room temperature for 24 hours, and the precipitate was removed. The filtrate was crystallized in an ether atmosphere to obtain crystals, namely Activate Anderson-type POM for carboxyl groups;
[0064] (3) Dissolve carboxyl-activated Anderson-type POM and D-His in anhydrous DMF at a ratio of 1:5, stir and re...
PUM
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