Method for synthesizing 1,2-diglyceride with an enzymic method

A technology of diglyceride and enzymatic synthesis, applied in the direction of fermentation, etc., can solve the problem of not being equal, and achieve the effect of mild reaction conditions, wide source of raw materials, and improved reaction efficiency

Active Publication Date: 2016-03-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The reaction takes more than 60 hours, and a large number of toxic reagents such as benzyl bromide, tetrabutylammonium bisulfate, and chloroform are introduced during the reaction, which is not an efficient and green synthetic route

Method used

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  • Method for synthesizing 1,2-diglyceride with an enzymic method
  • Method for synthesizing 1,2-diglyceride with an enzymic method
  • Method for synthesizing 1,2-diglyceride with an enzymic method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Add high oleic sunflower oil and n-propanol into the reaction kettle at a molar ratio of 1:30, put a stirring bar, preheat in a constant temperature water bath at a temperature of 40°C, turn on the magnetic stirrer, and stir at a speed of 500rpm After emulsifying for 15 minutes, add lipase LipozymeRMIM accounting for 6% of the total mass of the substrate to start the reaction. After 1 hour of reaction, take out the product and centrifuge at 4000 rpm for 10 minutes to remove the lipase. After HPLC analysis, the yield of 1,2-diglyceride in the product is was 45.3%.

Embodiment 2

[0035]Add high oleic sunflower oil and absolute ethanol into the reaction kettle at a molar ratio of 1:50, put a stirring bar, preheat it in a constant temperature water bath at a temperature of 50°C, turn on the magnetic stirrer, and set it at a speed of 500rpm After stirring and emulsifying for 15 minutes, lipase Lipozyme435 accounting for 6% of the total mass of the substrate was added to start the reaction. After 1 hour of reaction, the product was taken out, and the lipase was removed by centrifugation at 4000 rpm for 10 minutes. After HPLC analysis, 1,2-diglyceride in the product was obtained The rate is 90%.

Embodiment 3

[0037] Add palm oil and absolute ethanol to the reaction kettle at a molar ratio of 1:40, put a stirring bar, preheat in a constant temperature water bath at a temperature of 45°C, turn on the magnetic stirrer, stir and emulsify at a speed of 500rpm for 15min , add lipase Lipozyme435 accounting for 8% of the total mass of the substrate to start the reaction. After 1 hour of reaction, the product is taken out and centrifuged at 4000prm for 10 minutes to remove the lipase. According to HPLC analysis, the yield of 1,2-diglyceride in the product is 80.5%. .

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Abstract

The invention discloses a method for synthesizing 1,2-diglyceride with an enzymic method. The method comprises the steps of mixing vegetable oil with an organic solvent according to the mole ratio of 1:10 to 100, stirring, emulsifying, then adding immobilized lipase of which the mass is 2 to 20% of the total mass of substrates, reacting for 0.5 to 6 h at the temperature of 20 to 70 DEG C, and centrifuging to remove the lipase, so as to obtain a product which contains the 1,2-diglyceride. The synthetic method provided by the invention has the advantages of high yield, high efficiency, rapidity and convenience in industrial production and conforms to green chemistry, the reaction rate can be obviously increased, and the product is easy to purify.

Description

technical field [0001] The invention belongs to the technical field of synthesizing structural glycerides, in particular to a method for enzymatically synthesizing 1,2-diglycerides. Background technique [0002] Diacylglycerol (DAG) is a structural lipid formed by replacing a fatty acid in a triacylglycerol (TAG) with a hydroxyl group. There are mainly two isomers, 1,3-DAG and 1,2-DAG. , is generally recognized as safe (GRAS) food ingredients, but also a multifunctional additive, has broad application prospects in food, medicine, chemical and other industries. [0003] There are four main methods for the preparation of diglycerides (mainly referring to 1,3-diglycerides) developed at present: [0004] The first preparation method is disclosed in Japanese Patent No. 6-3438, which uses microorganisms of the genus Pseudomonas bacteria to catalyze the enzymatic hydrolysis and transesterification of solid oil and glycerin. The obtained oily product had 85% purity of diglycerides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 王小三祝雨筱王盈盈黄健花金青哲王兴国
Owner JIANGNAN UNIV
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