Plant mutagenic agent and preparation and use methods thereof
A mutagen and plant technology, applied in the direction of botany equipment and methods, plant gene improvement, application, etc., can solve the problems of limited scope of application and inability to realize plant polyploidy breeding, etc., and achieve simple and easy to use methods, improve Mutagenesis operation efficiency and good stability
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Embodiment 1
[0027] a plant mutagen
[0028] For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The mutagen is composed in parts by weight: 25kg of heavy water with a mass fraction of 65%, 25kg of nuclear reactor wastewater, 25kg of mining wastewater, 250kg of zirconium indium sand, 0.25kg of ethyl methanesulfonate, 0.3kg of colchicine, and rylene 0.2kg, fumingnong 0.2kg, heteroauxin 0.25kg, naphthalene ethane 0.25kg, ammonium dihydrogen phosphate 2.5kg, diammonium phosphate 2kg, potassium dihydrogen phosphate 2kg, potassium nitrate 3kg, ammonium nitrate 2kg, ethylene dihydrogen phosphate 3kg of amine tetraacetic acid, 2kg of diacetic acid triaminepentaacetic acid, 3.5kg of 1,2-cyclohexanediamine tetraacetic acid, 2.5kg of o-hydroxyphenylacetic acid, 2kg of hydroxyethyl ethylenediamine triacetic acid, 2...
Embodiment 2
[0030] a plant mutagen
[0031]For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The mutagen is composed of: 20kg of heavy water with a mass fraction of 60%, 20kg of nuclear reactor wastewater, 20kg of mining wastewater, 200kg of zirconium indium sand, 0.2kg of ethyl methanesulfonate, 0.2kg of colchicine, and rylene 0.1kg, fumingnong 0.1kg, heteroauxin 0.2kg, naphthalene ethane 0.1kg, ammonium dihydrogen phosphate 2kg, diammonium phosphate 1kg, potassium dihydrogen phosphate 1kg, potassium nitrate 2kg, ammonium nitrate 1kg, ethylenediamine Tetraacetic acid 1kg, diacetic acid triaminepentaacetic acid 1kg, 1,2-cyclohexanediaminetetraacetic acid 2kg, o-hydroxyphenylacetic acid 1kg, hydroxyethylethylenediaminetriacetic acid 1kg, tap water 2000kg.
Embodiment 3
[0033] a plant mutagen
[0034] For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The weight part of the mutagen consists of: 30kg of heavy water with a mass fraction of 70%, 30kg of nuclear reactor wastewater, 30kg of mining wastewater, 300kg of zirconium indium sand, 0.3kg of ethyl methanesulfonate, 0.4kg of colchicine, and rylene 0.3kg, fumingnong 0.3kg, heteroauxin 0.3kg, naphthalene ethane 0.4kg, ammonium dihydrogen phosphate 3kg, diammonium phosphate 3kg, potassium dihydrogen phosphate 3kg, potassium nitrate 4kg, ammonium nitrate 3kg, ethylenediamine Tetraacetic acid 5kg, diacetic acid triaminepentaacetic acid 3kg, 1,2-cyclohexanediaminetetraacetic acid 5kg, o-hydroxyphenylacetic acid 4kg, hydroxyethylethylenediaminetriacetic acid 3kg, tap water 3000kg.
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