Plant mutagenic agent and preparation and use methods thereof

A mutagen and plant technology, applied in the direction of botany equipment and methods, plant gene improvement, application, etc., can solve the problems of limited scope of application and inability to realize plant polyploidy breeding, etc., and achieve simple and easy to use methods, improve Mutagenesis operation efficiency and good stability

Inactive Publication Date: 2016-04-13
HUIAN KELIAN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot achieve polyploidy breeding of plants, and its scope of application is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] a plant mutagen

[0028] For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The mutagen is composed in parts by weight: 25kg of heavy water with a mass fraction of 65%, 25kg of nuclear reactor wastewater, 25kg of mining wastewater, 250kg of zirconium indium sand, 0.25kg of ethyl methanesulfonate, 0.3kg of colchicine, and rylene 0.2kg, fumingnong 0.2kg, heteroauxin 0.25kg, naphthalene ethane 0.25kg, ammonium dihydrogen phosphate 2.5kg, diammonium phosphate 2kg, potassium dihydrogen phosphate 2kg, potassium nitrate 3kg, ammonium nitrate 2kg, ethylene dihydrogen phosphate 3kg of amine tetraacetic acid, 2kg of diacetic acid triaminepentaacetic acid, 3.5kg of 1,2-cyclohexanediamine tetraacetic acid, 2.5kg of o-hydroxyphenylacetic acid, 2kg of hydroxyethyl ethylenediamine triacetic acid, 2...

Embodiment 2

[0030] a plant mutagen

[0031]For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The mutagen is composed of: 20kg of heavy water with a mass fraction of 60%, 20kg of nuclear reactor wastewater, 20kg of mining wastewater, 200kg of zirconium indium sand, 0.2kg of ethyl methanesulfonate, 0.2kg of colchicine, and rylene 0.1kg, fumingnong 0.1kg, heteroauxin 0.2kg, naphthalene ethane 0.1kg, ammonium dihydrogen phosphate 2kg, diammonium phosphate 1kg, potassium dihydrogen phosphate 1kg, potassium nitrate 2kg, ammonium nitrate 1kg, ethylenediamine Tetraacetic acid 1kg, diacetic acid triaminepentaacetic acid 1kg, 1,2-cyclohexanediaminetetraacetic acid 2kg, o-hydroxyphenylacetic acid 1kg, hydroxyethylethylenediaminetriacetic acid 1kg, tap water 2000kg.

Embodiment 3

[0033] a plant mutagen

[0034] For plant polyploid breeding and cultivation, the mutagen is divided into a solid part and a liquid part, the solid part is used for seed embedding, and the solid part is zirconium indium sand; the liquid part is used for seedling watering. The weight part of the mutagen consists of: 30kg of heavy water with a mass fraction of 70%, 30kg of nuclear reactor wastewater, 30kg of mining wastewater, 300kg of zirconium indium sand, 0.3kg of ethyl methanesulfonate, 0.4kg of colchicine, and rylene 0.3kg, fumingnong 0.3kg, heteroauxin 0.3kg, naphthalene ethane 0.4kg, ammonium dihydrogen phosphate 3kg, diammonium phosphate 3kg, potassium dihydrogen phosphate 3kg, potassium nitrate 4kg, ammonium nitrate 3kg, ethylenediamine Tetraacetic acid 5kg, diacetic acid triaminepentaacetic acid 3kg, 1,2-cyclohexanediaminetetraacetic acid 5kg, o-hydroxyphenylacetic acid 4kg, hydroxyethylethylenediaminetriacetic acid 3kg, tap water 3000kg.

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PUM

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Abstract

The invention discloses a plant mutagenic agent used for polyploid breeding and culturing of plants. The mutagenic agent is prepared from heavy water, nuclear reactor wastewater, mining wastewater, zirconium-indium sand, ethylmethane sulfonate, colchicine, acenaphthene, fumiron, heteroauxin, naphtho-ethane, ammonium dihydrogen phosphate, diammonium phosphate, monopotassium phosphate, potassium nitrate, ammonium nitrate, ethylenediaminetetraacetic acid, oxalic acid penteticacid, 1,2-cyclohexanediamine tetraacetic acid, 2-hydroxyphenylacetic acid, hydroxyethyl ethylenediamine triacetic acid and tap water. The plant mutagenic agent comprises a solid part and a liquid part; the solid part is used for embedding seeds, and the zirconium-indium sand serves as the solid part; and the liquid part is used for watering seedlings. The solid and liquid cooperating mutagenesis mode is adopted, so that the mutagenic effect is better; the zirconium-indium sand containing rich radioactive substances is adopted as a matrix for culturing seeds, so that the mutagenic efficiency is easily improved.

Description

technical field [0001] The invention belongs to the field of plant breeding, in particular to a plant mutagen and a method for making and using the same. Background technique [0002] The use of mutation technology to create useful variations has been proven to be a very promising breeding method. So far, 2,000 excellent mutant varieties have been selected from more than 150 kinds of plants all over the world for popularization and application in production. In vitro culture, double haploid technology is a more effective method to promote the application of mutation technology. With the deepening of research, the existing polyploid induction methods will be continuously improved, especially the most widely used chemical induction methods in production. However, most of the current mutagens are complicated to operate and use, and it is difficult to use them as conveniently as fertilization and irrigation. Therefore, it is necessary to develop a mutagen that can be easily u...

Claims

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Application Information

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IPC IPC(8): A01H1/08C05G3/00
CPCA01H1/08C05B7/00C05G3/00C05G5/23C05C1/00C05C5/02C05F7/005
Inventor 肖礼池
Owner HUIAN KELIAN AGRI TECH CO LTD
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