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Specific primer pair of Pantoea beijing and its application

A specific primer pair, pantoea technology, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of industrial production loss and difficult control of Pleurotus eryngii

Active Publication Date: 2019-03-08
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once bacterial soft rot of Pleurotus eryngii occurs in the production workshop, it is difficult to control, causing serious losses to the industrial production of Pleurotus eryngii

Method used

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  • Specific primer pair of Pantoea beijing and its application
  • Specific primer pair of Pantoea beijing and its application
  • Specific primer pair of Pantoea beijing and its application

Examples

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Embodiment 1

[0028] Example 1. Identification of Pantoea beijingensis using PCR primers for ALF-ALR

[0029] 1. PCR reagents for identifying Pantoea beijingensis

[0030]The PCR reagent for the identification of Pantoea Beijingi in this embodiment consists of the Pantoea Beijingi specific primer pair ALF-ALR, 2×Taq PCRMix (Bioeasy) and ddH 2 O composition.

[0031] Among them, the Pantoea beijing specific primer pair ALF-ALR is composed of ALF (upstream primer) and ALR (downstream primer), the nucleotide sequence of ALF is 5'ATCTCTCAGACAAGCCCCGTTAGTAACAACT 3'(SEQ ID No.1), the nucleotide sequence of ALR The sequence is 5'GATTTTCCTTCACATATTCCACTGCACGAG 3' (SEQ ID No. 2).

[0032] 2. Identification of Pantoea beijingensis

[0033] 2.1. DNA extraction of strains

[0034] Strains preserved from -80°C (Pantoea beijingensis sp.nov.) JZB2120001 T Pantoea gaviniae DSM22758; Pantoea eucalypti DSM23077; Pantoea anthophila DSM23080; T ) was streaked in solid LB medium, cultivated at 28°C for 20...

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Abstract

The invention discloses a pantoea beijingensis specific primer and application thereof. An antoea beijingensis specific primer pair ALF-ALR is composed of a primer ALF and a primer ALR. The primer ALF is a single-strand DNA molecule shown as SEQ ID No.1. The primer ALR is a single-strand DNA molecule shown as SEQ ID No.2. The antoea beijingensis specific primer pair ALF-ALR only amplifies a specific band in genome DNA of pantoea beijingensis, no amplification products exist in other bacterial strains, and the antoea beijingensis specific primer pair ALF-ALR can be used for quickly identifying pantoea beijingensis.

Description

technical field [0001] The invention relates to a pair of specific primers for Pantoea beijing and its application in the field of biotechnology. Background technique [0002] my country began large-scale cultivation of Pleurotus eryngii in the 1990s. Pantoea includes several species that act as plant growth promoters, biocontrols or plant pathogens (Pantoea: insights into a highly versatile and diversegenus within the Enterobacteriaceae, FEMS Microbiology Reviews (2015) 39:968-984). The bacterial soft rot of Pleurotus eryngii caused by Pantoea is a serious disease that has occurred in the industrial production of Pleurotus eryngii at home and abroad in recent years. The pathogenic bacteria of Pleurotus eryngii soft rot reported in Beijing was identified and named as Pantoea beijingensis sp. nov., isolated from the fruiting body of Pleurotus eryngii. Liu et al. Antonie van Leeuwenhoek (2013) 104:1039–1047). Once bacterial soft rot of Pleurotus eryngii occurs in the product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/01
CPCC12Q1/689
Inventor 荣成博刘宇马元伟王守现许峰宋婧祎李瑞杰赵爽黄斌王晶王兰青宋爽牛玉蓉
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES