Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of extraction method of salvianolic acid components in Salvia miltiorrhiza stems and leaves

A technology for extracting stems and leaves of Salvia miltiorrhiza, applied in the field of natural product extraction, can solve the problems of unsuitability for industrial application, low efficiency of wall breaking, and high cost of a single enzyme, and achieve the effects of good product quality, reduced production cost, and accelerated release

Inactive Publication Date: 2019-11-26
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the plant cell wall is a lignocellulose composite macromolecule composed of lignin, cellulose, and hemicellulose, the efficiency of a single enzyme in breaking the wall is often not high, and the combined action of multiple enzyme systems is often required to effectively degrade the cell wall.
The compound enzyme preparations currently on the market are mainly fermented by multiple strains to produce a single enzyme, and then the product is formed through compounding technology. For many enzyme preparation manufacturers, the conditions are relatively high, and the cost of a single enzyme is too high, so Not suitable for large-scale industrial applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of extraction method of salvianolic acid components in Salvia miltiorrhiza stems and leaves
  • A kind of extraction method of salvianolic acid components in Salvia miltiorrhiza stems and leaves
  • A kind of extraction method of salvianolic acid components in Salvia miltiorrhiza stems and leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting salvianolic acid components in stems and leaves of salvia miltiorrhiza, comprising the following steps: drying the stems and leaves of salvia miltiorrhiza, pulverizing into 40-mesh powder, weighing 50 g, adding compound enzyme crude enzyme liquid according to the enzyme-material ratio of 15:1, shaking After homogenization, control the enzymolysis temperature at 40°C, pH value 4.5-5.0, enzymolysis time 0.5 hours, and process the stems and leaves of Salvia miltiorrhiza; after the enzymolysis is completed, add 50 times the amount of distilled water, put it in a water bath at 50°C, and keep warm Extract by water immersion for 90min and then filter. The filter residue was extracted with pure water for the second time; the two extracts were combined. Concentrate under reduced pressure at 45°C to obtain a concentrated solution of phenolic acids in stems and leaves of Salvia miltiorrhiza; the concentrated solution is then freeze-dried under the following...

Embodiment 2

[0036] A method for extracting salvianolic acid components in stems and leaves of salvia miltiorrhiza, comprising the following steps: drying stems and leaves of salvia miltiorrhiza, pulverizing into 40-mesh powder, weighing 50 g, adding compound enzyme crude enzyme liquid according to the enzyme-material ratio of 20:1, shaking After uniformity, control the enzymolysis temperature at 30°C, pH value 4.5-5.0, and enzymolysis time for 1 hour, and process the stems and leaves of Salvia miltiorrhiza. Extract by water immersion for 90min and then filter. The filter residue was extracted with pure water for the second time; the two extracts were combined. Concentrate under reduced pressure at 45°C to obtain a concentrated solution of phenolic acids in stems and leaves of Salvia miltiorrhiza; the concentrated solution is then freeze-dried under the following operating conditions: vacuum degree of 135 bar and temperature of -45°C to obtain dry powder of the water-soluble extract of Sal...

Embodiment 3

[0038] A method for extracting salvianolic acid components in stems and leaves of salvia miltiorrhiza, comprising the following steps: drying stems and leaves of salvia miltiorrhiza, pulverizing into 40-mesh powder, weighing 50 g, adding compound enzyme crude enzyme liquid according to the enzyme-material ratio of 18:1, shaking After uniformity, control the enzymolysis temperature at 50°C, pH value 4.5-5.0, enzymolysis time 45min, and process the stems and leaves of Salvia miltiorrhiza; After extraction for 90min, filter. The filter residue was extracted with pure water for the second time; the two extracts were combined. Concentrate under reduced pressure at 45°C to obtain a concentrated solution of phenolic acids in stems and leaves of Salvia miltiorrhiza; the concentrated solution is then freeze-dried under the following operating conditions: vacuum degree of 135 bar and temperature of -45°C to obtain dry powder of the water-soluble extract of Salvia miltiorrhiza.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting phenolic acid ingredients in salvia miltiorrhiza stems and leaves. The method is characterized by including the steps of complex enzyme extraction and water extraction, wherein complex enzyme is a crude enzyme fluid produced through Psathyrella candolleana BDF15 fermentation, Psathyrella candolleana BDF15 is preserved at the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC, and the address is Courtyard 1 No.3 of Beichen west road of Chaoyang District of Beijing) of China Committee for Culture Collection of Microorganisms on November 4, 2015, and the preservation number is CGMCC No.11415. The extracting method can effectively promote release of the phenolic acid ingredients in the salvia miltiorrhiza stems and leaves, improve the extraction efficiency and reduce the production cost. In addition, no harmful substance is produced in the production process, no pollution is produced, the product quality is good, and the effect is strong. Furthermore, traditional Chinese medicine waste is also comprehensively utilized and is turned into wealth, and the method is an environmentally-friendly green extraction method.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting salvianolic acid components in stems and leaves of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza Bunge is a plant of the genus Salvia in the Labiatae family. It has the functions of dispelling blood stasis and relieving pain, promoting blood circulation and promoting menstrual flow, clearing the heart and eliminating troubles, and treating cardiovascular diseases. The main active substances of Danshen are fat-soluble tanshinones and water-soluble salvianolic acids. Since the root of Danshen is mostly used as medicine, the root of Danshen and its preparations are widely used clinically. The annual output of Danshen Dao’s production area is about 10 million kilograms, but a large number of aerial parts are discarded. Therefore, in the process of processing Danshen root, it will cause A lot of waste. S...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/537
CPCA61K36/537A61K2236/19A61K2236/331A61K2236/51A61K2236/53
Inventor 李艳玲常正尧史仁玖张显忠
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI