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A rice non-endosperm expression promoter safes3 and its application

A non-endosperm, promoter technology, applied in the field of biotechnology and crop genetic engineering, can solve the problem that research results have no application value, and achieve the effect of reducing food safety risks, improving growth performance, and helping commercial promotion.

Active Publication Date: 2019-08-13
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] If transgenic rice cannot be commercialized and used in production, no matter how good the research results are, they will have no application value

Method used

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  • A rice non-endosperm expression promoter safes3 and its application
  • A rice non-endosperm expression promoter safes3 and its application
  • A rice non-endosperm expression promoter safes3 and its application

Examples

Experimental program
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Embodiment 2

[0057] In order to verify that the sequence in SEQ ID No: 2 of the present invention also has the same function, the present invention adopts the similar scheme in Example 1, but changes the primer sequence, and conducts the same experiment as Example 1.

[0058] It has been verified by experiments that although the transformation is lower than in Example 1, the specific expression of Gus gene in non-endosperm parts can still be induced. It was confirmed that the sequence shown in SEQ ID No: 2 has a promoter function similar to that of SEQ ID No: 1.

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Abstract

The invention provides a rice non-endosperm expression promoter SAFES3 and its application. The invention also provides an expression cassette and a plant expression vector containing the promoter. Specifically, the present invention applies the above-mentioned promoters to crop genetic engineering. The promoter provided by the invention can specifically drive the expression of exogenous genes in non-endosperm tissues of rice, and utilize the characteristics of non-endosperm expression promoters to give full play to the advantages of transgenic breeding and breed more and better new varieties. It is verified by experiments that the promoter of the present invention can drive the expression of foreign genes in non-edible parts (non-endosperm) of rice without causing expression in edible parts. Once this kind of promoter is widely used, it will be very easy to promote, and it will try to ensure that the quality of rice will not be affected by genetic modification, and it will be more conducive to eliminating public doubts about genetically modified products.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and crop genetic engineering. Specifically, the present invention relates to a rice non-endosperm expression promoter and its application. Endosperm structural properties for the purpose of improving rice traits. Background technique [0002] Rice is one of the most important crops, and more than one-third of the world's population uses rice as a staple food. In my country, the production area of ​​rice accounts for about 40% of the crop planting area, and rice production occupies a very important position in the national economy. Due to the constant changes in the natural environment and the needs of economic development, some practical production problems that are difficult to solve by conventional breeding techniques, such as insect pests, diseases and adversity stress, need to be solved by modern biotechnology. Transgenic technology can modify the genetic material of plants at the ge...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N15/84A01H5/00A01H6/46
CPCC07K14/415C12N15/8205C12N15/8225C12N15/8226C12N15/8227
Inventor 秦瑞英杨剑波李莉魏鹏程李浩李娟杨亚春许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI