Chip applicable to electrochemical real-time PCR and preparation method of chip
A kind of electrochemical and chip technology, applied in the field of biochip research, to achieve the effect of improving detection throughput, strong specificity and high sensitivity
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[0035] Below in conjunction with accompanying drawing, further illustrate the present invention through embodiment.
[0036] see Figure 1 ~ Figure 3 , the preparation method of electrochemical real-time PCR chip of the present invention, comprises the steps:
[0037] Step 1: Take a substrate 101, clean and remove organic and inorganic impurities on the surface of the substrate 101, and perform vacuum drying.
[0038]The substrate is a silicon wafer, which is cleaned by ultrasonic waves in analytical pure acetone and deionized water to remove organic impurities on the surface of the silicon wafer, then heated and boiled with a mixture of concentrated sulfuric acid and hydrogen peroxide, and rinsed with deionized water. Remove the inorganic impurities on the surface of the silicon wafer, dry the cleaned silicon wafer with nitrogen, put it into a petri dish, and then put it in a vacuum drying oven to dry at 120°C. The purpose of this operation is to remove the water vapor attac...
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