Chip applicable to electrochemical real-time PCR and preparation method of chip

A kind of electrochemical and chip technology, applied in the field of biochip research, to achieve the effect of improving detection throughput, strong specificity and high sensitivity

Inactive Publication Date: 2016-05-25
PEKING UNIV +1
View PDF8 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no chip capable of electrochemical real-time PCR in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chip applicable to electrochemical real-time PCR and preparation method of chip
  • Chip applicable to electrochemical real-time PCR and preparation method of chip
  • Chip applicable to electrochemical real-time PCR and preparation method of chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] Below in conjunction with accompanying drawing, further illustrate the present invention through embodiment.

[0036] see Figure 1 ~ Figure 3 , the preparation method of electrochemical real-time PCR chip of the present invention, comprises the steps:

[0037] Step 1: Take a substrate 101, clean and remove organic and inorganic impurities on the surface of the substrate 101, and perform vacuum drying.

[0038]The substrate is a silicon wafer, which is cleaned by ultrasonic waves in analytical pure acetone and deionized water to remove organic impurities on the surface of the silicon wafer, then heated and boiled with a mixture of concentrated sulfuric acid and hydrogen peroxide, and rinsed with deionized water. Remove the inorganic impurities on the surface of the silicon wafer, dry the cleaned silicon wafer with nitrogen, put it into a petri dish, and then put it in a vacuum drying oven to dry at 120°C. The purpose of this operation is to remove the water vapor attac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a chip applicable to electrochemical real-time PCR and a preparation method of the chip. The preparation method comprises the following steps: growing an insulating layer on a substrate, and then preparing three electrodes including a working electrode, an auxiliary electrode and a reference electrode; then growing an insulating film on the surface of the working electrode, and transferring a mask pattern from a microarray electrode to the insulating film by virtue of a photoetching process and an etching process; and etching a region of the insulating film on the working electrode, so as to generate the microarray electrodes. Each microarray electrode is an independent reaction unit and is applied to electrochemical real-time PCR. The chips can be combined in series or in parallel for use and can be used for simultaneously carrying out multiple PCRs, so that the detection throughput is greatly increased; the chip has the advantages of being high in precision and throughput and capable of realizing low-concentration quantitative detection.

Description

technical field [0001] The invention relates to the field of biochip research, in particular to a chip that can be used for electrochemical real-time PCR and a manufacturing method thereof. Background technique [0002] Polymerase Chain Reaction (Polymerase Chain Reaction, referred to as PCR), is a method of enzymatically synthesizing specific DNA fragments in vitro. DNA can be rapidly amplified, and has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. It can not only be used in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also in medical, food and health fields such as pathogen detection, prenatal diagnosis, tumor gene detection, efficacy evaluation, food safety detection, and food genetically modified detection. [0003] The number of DNA copies produced during the PCR reaction increases exponentially. With the increase of the number of reaction cycles, the final PCR reaction no longer ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30
Inventor 陆祖宏李清宁李成强严勇魏颖颖孙利王磊孙杰
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap