Method for preparing cowhide collagen by complex enzymatic hydrolysis

A compound enzyme method and compound protease technology, which is applied in the field of preparation of cowhide collagen by compound enzymatic hydrolysis, can solve problems such as application limitations of enzymatic hydrolyzed protein, and achieve the effects of saving procedures, increasing procedures, and reducing energy consumption

Active Publication Date: 2016-06-08
山东恒鑫生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This severely limits the application of enzymatically hydrolyzed proteins in the food industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 5kg of cowhide and wash it, add it to a cooking pot, add an appropriate amount of water, and adjust the pH value to 8 with calcium hydroxide. Heat up to 65°C and keep warm, according to 8x10 6 The ratio of U / kg substrate was added to the complex enzyme (papain: trypsin: Bacillus subtilis alkaline protease=3:2:1) for 2 hours of hydrolysis. Enzymatic hydrolysis and neutralization. After the enzymatic hydrolysis is completed, remove the visible oil drift on the surface of the feed liquid. Raise the temperature to 90°C for 5 minutes. Release the primary glue solution, pour it into a decolorization and debittering tank, add 0.05kg of activated carbon for decolorization and debittering, and heat up to 90°C for 30 minutes. The feed liquid is filtered through a plate and frame filter and then enters the storage tank. After cooling the feed liquid in the storage tank to 60°C, the feed liquid can enter the ion exchanger for ion exchange. The glue feeding speed is control...

Embodiment 2

[0035] Take 10kg of cowhide and wash it, put it into a cooking pot, add an appropriate amount of water, and adjust the pH value to 8 with calcium hydroxide. Heat up to 65°C for heat preservation, according to 10x10 6 The ratio of U / kg substrate was added to the complex enzyme (papain: trypsin: Bacillus subtilis alkaline protease=3:2:1) for 6 hours of hydrolysis. Enzymatic hydrolysis and neutralization. After the enzymatic hydrolysis is completed, remove the visible oil drift on the surface of the feed liquid. Raise the temperature to 90°C for 5 minutes. Release the primary glue solution, put it into a decolorization and debittering tank, add 0.1kg of activated carbon for decolorization and debittering, and heat up to 100°C for 1 hour. The feed liquid is filtered through a plate and frame filter and then enters the storage tank. After cooling the feed liquid in the storage tank to 60°C, the feed liquid can enter the ion exchanger for ion exchange. The glue feeding speed is ...

Embodiment 3

[0037] Take 8.5kg of cowhide and wash it, put it into a cooking pot, add an appropriate amount of water, and adjust the pH value to 8 with calcium hydroxide. Heat up to 65°C for heat preservation, according to 9x10 6 The ratio of U / kg substrate was added to the compound enzyme (papain: trypsin: Bacillus subtilis alkaline protease=3:2:1) for 10 hours of hydrolysis. Enzymatic hydrolysis and neutralization. After the enzymatic hydrolysis is completed, remove the visible oil drift on the surface of the feed liquid. Raise the temperature to 90°C for 5 minutes. Release the primary glue solution, pour it into a decolorization and debittering tank, add 0.085kg of activated carbon for decolorization and debittering, and heat up to 90°C for 1 hour. The feed liquid is filtered through a plate and frame filter and then enters the storage tank. After cooling the feed liquid in the storage tank to 60°C, the feed liquid can enter the ion exchanger for ion exchange. The glue feeding speed...

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Abstract

The invention discloses a method for preparing cowhide collagen by complex enzymatic hydrolysis. A process for preparing cowhide collagen by complex enzymatic hydrolysis is used to acquire a required protein product. The method comprises steps of cowhide soaking and washing, cooking enzymatic hydrolysis, decoloring and debitterizing, filtering, ion exchanging, concentrating, sterilizing, spray drying, and screening. The complex process of a traditional manufacture technique is omitted, reducing energy consumption and cost, it is possible to prepare collagen by directly using cowhide, the obtained collagen is good in quality, has a molecular weight between 3050-3300 Da and has bitter taste greatly reduced.

Description

technical field [0001] The invention relates to a process for preparing cowhide collagen by compound enzymatic hydrolysis, which belongs to the field of biotechnology. Background technique [0002] In the industrial production of gelatin, one of the main raw materials is cowhide, and the production process is complicated. The raw material of hydrolyzed collagen is gelatin according to the requirements of industry standards. Over the years, various processes have been developed for extracting collagen from gelatin. Such as adopting clear water high-temperature cooking, acid hydrolysis, and alkali hydrolysis, these methods all have relatively large disadvantages, which are not conducive to production. Collagen molecules are heated and denatured, causing random hydrolysis of the bonds in the collagen, and easily turning into macromolecular gelatin. The acid hydrolysis method will destroy some amino acids, and the product yield is low, the process conditions are high and seco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 曹祥来
Owner 山东恒鑫生物科技有限公司
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