Preparation method of control material for blood cell analyzer

A technology for blood cell analyzer and quality control product, which is applied in the field of preparation of blood cell analyzer quality control product, can solve the problems of large economic burden, high cost and poor stability of patients, and achieves great market promotion value, convenient transportation and high stability. sexual effect

Inactive Publication Date: 2016-06-08
THE FIRST AFFILIATED HOSPITAL OF XIAN JIAOTONG UNIV
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  • Abstract
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Problems solved by technology

At present, due to the poor stability of domestic whole blood quality control products, imported whol

Method used

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  • Preparation method of control material for blood cell analyzer
  • Preparation method of control material for blood cell analyzer

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Embodiment Construction

[0020] A kind of preparation method of hematology analyzer quality control article of the present invention, such as figure 1 shown, including the following steps:

[0021] (1) Separate the white blood cells and platelets from the whole blood of normal healthy people by using the buffy coat method; first separate the red blood cells and fix them with a fixative, and then centrifuge the buffy coat and plasma again to separate the white blood cells and platelets;

[0022] (2) Take the leukocytes and platelets separated in step (1), add a fixative therein, and wash the fixation after they are fixed;

[0023] (3) Add cell maintenance solution to the fixed species after washing, which is a high-value quality control product; and divide it into 3 equal parts;

[0024] (4) Take any two of the 3 quality control products obtained in step (3) and add blood cell maintenance solution to make a white blood cell quintile medium-low value quality control product;

[0025] (5) After it is s...

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Abstract

A preparation method of a control material for a blood cell analyzer belongs to the field of clinical medical supplies. The method is characterized by comprising the steps of: separating leukocytes and platelets of healthy human whole blood by using an albuginea method; fixing the isolated erythrocytes by a fixative, adding a fixative into the isolated leukocytes and platelets, and washing the fixture; adding a cell maintenance liquid to the fixture after washing; after stabilization, subpackaging the quality control material in sterile small glass bottles for storage, so as to complete preparation of quality control materials. The method uses fresh whole blood as the raw material, and applies formaldehyde and glutaraldehyde with different concentrations as fixatives; the added maintenance fluid maintains the original cell morphology; the control material has high stability, and advantages of low price, convenience in transportation compared to imported original control products, and shows great marketing value.

Description

technical field [0001] The invention belongs to the field of medical clinical supplies, and in particular relates to a preparation method of a quality control product of a blood cell analyzer. Background technique [0002] With the continuous development of science and technology, all kinds of medical equipment have been applied to the actual treatment process, especially in recent years, with the improvement of people's living standards, the emphasis on their own health has been strengthened, and the physical examination and other aspects The application of medical equipment is more extensive, among which, blood cell analyzer is one of the widely used. Because the changes of many diseases can be reflected by the changes in the number and shape of blood cells, the health status of the body can be judged through the results of cell testing, and therefore, the accuracy of cell testing results is very important. The quality control product is used to monitor whether the hemato...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N33/48
CPCG01N1/28G01N33/48
Inventor 李鹏袁晓红李雯陈静张祎
Owner THE FIRST AFFILIATED HOSPITAL OF XIAN JIAOTONG UNIV
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