A kind of tissue culture rapid propagation method of Maoba Damuqi

Abstract

The invention relates to a tissue culture rapid-propagation method of Maoba wild sumac. Fresh axillalry buds and terminal buds of five-year excellent Maoba wild sumac are used as explant culture materials and undergo steps of disinfection, induced culture, proliferation culture, rooting culture, hardening-seedling, transplantation and the like. Rapid propagation of Maoba wild sumac is realized through the tissue culture rapid-propagation technology. For each 10 Maoba wild sumac explants, propagation coefficient is 6.5, and propagation cycle is 30 days. Except pollution loss, yearly propagation rate reaches 2.75 million and above. Thus, the problem that Maoba wild sumac is scarce is effectively solved, and the requirement of large-area rapid propagation is met. By accurate selection of explant picking time and tree-age, induced differentiation rate of adventitious buds is raised, and the differentiation rate reaches 85% and above. In addition, browning rate of explants is lower than 8%, pollution rate is lower than 2%. By adoption of closed hardening-seedling and reasonable matrix proportion, survival rate of seedlings is greatly raised, and the survival rate is maintained at 90% and above.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of Maoba damuqi. Background technique [0002] Raw lacquer is a water-in-oil milky white liquid that flows out of the phloem of the lacquer tree, and is a physiological secretion of the lacquer tree. Raw lacquer is a renewable resource of my country's special product, and it is also a traditional native product with a long history. Its output accounts for more than 80% of the world's total. Hubei is a large lacquer-producing province in China. Maoba lacquer was formed under specific climate and altitude conditions. In 1954, Premier Zhou Enlai awarded the "ba lacquer famous all over the world". For this reason, Maoba lacquer became famous all over the country and even the world traditional brand. Maoba raw lacquer is famous at home and abroad for its thick lacquer quality, bright color, fragrant s...

AI Technical Summary

Problems solved by technology

Because sumac is an economic tree species of cross-pollination, it will produce greater differentiation when propagated by seeds, and the genetic characteristics of its fine varieties are difficult to maintain; seedlings are cultivated by burying roots, except for a very small number of possible bud changes, which are stable and not easy to degenerate. The seedling rate is low, the propagation speed is slow, and the fine varieties and fine single plants of sumac are restricted

The tissue culture rapid propagation technology can well ensure the excellent varieties of sumac and the genetic stability of a single plant, and can meet the large-scale propagation requirements. In the prior art, there are reports about the tissue culture rapid propagation technology being applied to the sumac propagation, such as , the patent application number is 201410311305.4 discloses a kind of high-efficiency and rapid multiplication method of small wood lacquer, including (1) selection and disinfection of explants (2) initiation culture (3) proliferation culture (4) rooting culture (5) seedling hardening With the transplanting step, the method of tissue culture of a fine single plant of fine wood paint can be used to make its reproduction coefficient reach 4 to 5 times. For every 10 effective small wood paint axillary buds, the reproduction coefficient is 4.5, and the multiplication cycle is 45 days. , except for pollution loss, its annual breeding capacity will still reach more than 1.75 million, which effectively solves the problem of rapid propagation of small wood lacquer and enables large-scale promotion of high-quality clone materials, but the invention has a long breeding cycle and a low reproduction coefficient. Moreover, the cultivated small wood paint is easy to be polluted, browned, and has a high loss rate, so it is not suitable for the tissue culture and rapid propagation of Maoba large wood paint

In addition, the patent application with the application number 201410540495.7 discloses a method for rapid propagation of sumac regenerated plants, including steps such as disinfection of sumac leaves, callus induction, callus differentiation, rooting induction, seedling hardening and transplanting, etc. The invention selects sumac leaves as explants to avoid browning, which will lead to low induction differentiation rate