A kind of induction medium formula for tomato flower cultivation
A medium formula and tomato flower technology, which is applied in the field of agricultural science, can solve the problems of unclear interaction between medium components and culture conditions, low induction rate and differentiation rate of embryoid bodies or callus, and gaps.
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Embodiment 1
[0015] Prepare the culture medium with the following formula:
[0016] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙H 2 O 1200mg / L, CaCl 2 ∙2H 2 O225mg / L, Na 2 -EDTA 75mg / L, FeSO 4 ∙7H 2 O 55mg / L, MnSO 4 ∙ H 2 O 17mg / L, ZnSO 4 ∙7H 2 O 18mg / L, H 3 BO 3 6.0mg / L, KI 0.8mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl∙6H 2 O 0.03mg / L, NaMoO 4 ∙2H 2 O0.25mg / L, AgNO 3 8.0mg / L, inositol 100mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;
[0017] 2,4-D 1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH 5.8.
[0018] Tomato cultivars Dayezaofen and Momotaro were selected as donors for anther culture, and flower buds in full bloom were taken at 9:00-11:00 am on a sunny day, and the flower buds were washed with washin...
Embodiment 2
[0021] Prepare the medium with the following formula (AgNO 3 Adding the preferred concentration):
[0022] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙H 2 O 1200mg / L, CaCl 2 ∙2H 2 O225mg / L, Na 2 -EDTA 75mg / L, FeSO 4 ∙7H 2 O 55mg / L, MnSO 4 ∙H 2 O 17mg / L, ZnSO 4 ∙7H 2 O 18mg / L, H 3 BO 3 6.0mg / L, KI 0.8mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl∙6H 2 O 0.03mg / L, NaMoO 4 ∙2H 2 O0.25mg / L, inositol 100mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, glycine 2.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3 g / L, maltose 20g / L, sucrose 15g / L, vegetable gel 5.0g / L;
[0023] 2,4-D 1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH 5.8.
[0024] AgNO 3 The added concentration is set in the following five types: 0; 2.0mg / L; 4.0mg / L; 6.0mg / L; 10.0mg / L.
[0025] The two tomato varieties were also selected as anther donors, the ...
Embodiment 3
[0027] Prepare the medium with the following formula (the preferred concentration of glycine added):
[0028] KNO 3 950mg / L, NH 4 NO 3 825mg / L, KH 2 PO 4 675mg / L, MgSO 4 ∙H 2 O 1200mg / L, CaCl 2 ∙2H 2 O225mg / L, Na 2 -EDTA 75mg / L, FeSO 4 ∙7H 2 O 55mg / L, MnSO 4 ∙H 2 O 17mg / L, ZnSO 4 ∙7H 2 O 18mg / L, H 3 BO 3 6.0mg / L, KI 0.8mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl∙6H 2 O 0.03mg / L, NaMoO 4 ∙2H 2 O0.25mg / L, AgNO 3 8.0mg / L, inositol 100mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 5.0mg / L, folic acid 0.5mg / L, cyclic guanosine monophosphate 0.3g / L, maltose 20g / L L, sucrose 15g / L, plant gel 5.0g / L;
[0029] 2,4-D 1.0mg / L, kinetin 0.4mg / L, complex nucleic acid 0.3mg / L, biotin 0.05mg / L, vitamin C 45μmol / L, activated carbon 175mg / L, hydrolyzed protein 0.6g / L, pH 5.8.
[0030] The added concentration of glycine is set in the following four types: 0; 1.0mg / L; 3.0mg / L; 4.0mg / L.
[0031] The two tomato varieties were also selected as anther donors, the operati...
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