A kind of microfluidic chip and its preparation method and application

A microfluidic chip and microchannel technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, biochemical instruments, etc., can solve problems such as no substantial breakthroughs, and achieve high-content drug combination Effects of screening, reduced usage, small sample and reagent usage

Active Publication Date: 2018-11-09
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, microfluidic chips are used to prepare microstructures and microchannels on the surface, relying on the mixing effect between liquids in the microchannels to drive multiple drugs to perform multi-level mixing, and at the same time complete cell culture and detection. There has not been a substantial breakthrough in the application field of screening

Method used

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  • A kind of microfluidic chip and its preparation method and application
  • A kind of microfluidic chip and its preparation method and application
  • A kind of microfluidic chip and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A microfluidic chip, the microfluidic chip is composed of two concentration gradient generating units 5, 18, 1 to 10 mixing units and cell culture units, the inlet of the mixing unit is the drug solution outlet 6 of the concentration gradient generating unit , 17, the uppermost end of the concentration gradient generating unit is drug solution or medium inlet 1, 2, 3, 4;

[0035] The concentration gradient generation unit 5 is a tree-like structure with branches added layer by layer from top to bottom, the channel of each branch is a circuitous zigzag shape, and the number of the most terminal branches is half of the number of mixing units. And each of its outlets is connected to the uppermost end of the mixing unit through a microchannel,

[0036] Described mixing unit is two microchannels of concentration gradient unit terminal channel branch, mixing unit fast microchannel 8 and mixing unit slow microchannel 10, and mixing unit fast microchannel is a long straight cha...

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Abstract

The invention provides a microfluidic chip and its preparation method and application. The microfluidic chip is composed of 2 concentration gradient generating units, 1 to 10 mixing units and cell culture units, and the entrance of the mixing unit is a concentration gradient generating unit. The drug solution outlet of the unit, the uppermost end of the concentration gradient generation unit is the drug solution or culture medium inlet. The microfluidic chip is integrated with a concentration gradient generating unit and a mixing unit, which can finally generate different levels of concentration mixing of various drugs to stimulate cells fixed in a specific cell culture unit, and it is possible to visually study the effect of cells during growth under a microscope. The reaction of multi-drug multi-level combination provides new ideas and new research technologies for large-scale drug combination screening. It is mainly used in related fields such as cell biology, genetics and drug screening. A new tool for cell analysis.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, in particular to a microfluidic chip and its preparation method and application. Background technique [0002] Cell culture is an indispensable and important step in the drug development process. Conventional cell culture has many deficiencies in high-throughput drug screening, and it is rarely involved in multi-level drug combination screening. There is no high-throughput screening platform. The multi-level combination of multiple drugs can more truly reflect the actual drug use of people, study the impact of drug combination on cells, and simulate the interaction between drugs in human tissues, which is also conducive to the rapid screening of the efficacy and effectiveness of new drug drug combination. toxicity. Therefore, the development of a convenient, fast, efficient, and low-cost drug combination screening technology is an urgent need in the fields of drug screening and cell ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
Inventor 秦建华李中玉许慧
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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