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Integrated microfluidic chip for drug screening and application thereof

A microfluidic chip and drug technology, applied in the field of cell biology, can solve the problems of reagent consumption, inability to simulate blood flow, continuous supply of medicinal liquid, etc., so as to reduce the number of cells and reagent consumption, realize drug screening, and avoid mutual the effect of interference

Pending Publication Date: 2019-05-07
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But what follows is that the above method cannot maintain a long-term concentration gradient, cannot be expanded to share a device for multiple biological types, and cannot accurately generate the required single concentration or mixed concentration, etc.
In addition, the research of some anti-malignant tumor drugs on cancer cells requires a lot of time and equipment, and is accompanied by huge consumption of reagents, and traditional cell culture methods cannot simulate the blood flow in the body and the continuous supply of liquid medicine

Method used

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  • Integrated microfluidic chip for drug screening and application thereof
  • Integrated microfluidic chip for drug screening and application thereof
  • Integrated microfluidic chip for drug screening and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] An integrated microfluidic chip for drug screening, which is a PDMS single-layer chip, specifically as Figure 1-3 As shown, it is composed of the upper fluid layer 1 and the lower glass layer 2, wherein the fluid layer 1 is a fluid channel made of PDMS, which is bonded with the lower glass layer 2 through a thin PDMS layer to form a fluid channel unit; There is a concentration gradient structure generation area, in which the liquid flow crosses and splits to generate a corresponding concentration gradient. The concentration gradient structure generation area is provided with a hexagonal fluid channel 3, and 3 sample injections are arranged at equal intervals on the hexagonal fluid channel 3. Inlet 4, each inlet 4 is opened at a vertex of the hexagonal fluid channel 3; the inlet 4 is used for injecting the drug solution to be tested and the cell culture solution.

[0035] The periphery of the hexagonal fluid channel 3 is equidistantly provided with 6 first microchannels...

Embodiment 2

[0042] The preparation method of the integrated microfluidic chip used for drug screening in Example 1 is as follows:

[0043] Step 1, design and print the structure: design the structure of the microfluidic chip through AutoCAD software, and then print it on the transparent mask by printing technology;

[0044] Step 2, making a chip template: Clean the silicon wafer with a mixed solution of hydrogen peroxide and concentrated sulfuric acid with a volume ratio of 1:3 for more than 15 minutes, and then wash the silicon wafer sequentially in the order of absolute ethanol, acetone and ultrapure water; Afterwards, the silicon wafer is dried on a heating plate, and then the surface of the silicon wafer is modified with hexamethyldisiloxane to make the photoresist stable and not easy to be washed off. In the machine, pour Az-50XT photoresist to the center of the silicon plate, shake the photoresist to a suitable height at a speed of 2000rpm, and then perform ultraviolet exposure, tha...

Embodiment 3

[0048] The integrated microfluidic chip for drug screening prepared in Example 2 is used for breast tumor MCF-7 cell culture, specifically including the following steps:

[0049] Select the microfluidic chip in Example 2, and use a precision syringe pump to transfer 2 μL of cells to a density of 1×10 6 ml -1 The breast tumor MCF-7 cell suspension was injected into the corresponding cell culture chamber 11 through one of the cell injection ports 12, and a precision syringe pump was used to apply a driving force so that the injection speed of the cell suspension was 2.0 μl·min -1 ; After the MCF-7 cell suspension is filled with the cell culture chamber 11, the precision syringe pump is removed to keep the cell suspension in the cell culture chamber 11 in a static state, and then the microfluidic chip is placed in CO 2 Culture in a cell incubator; after 12 hours of culture, the cells adhere to the wall, and then take photos of the cells to observe the growth status of the cells....

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Abstract

The invention discloses an integrated microfluidic chip for drug screening. The chip is formed by a flow layer and a glass layer; the flow layer is provided with a concentration gradient structure generation region; the concentration gradient structure generation region is provided with a hexagonal fluid channel; the hexagonal fluid channel is provided with three sample introduction ports; the periphery of the hexagonal fluid channel is provided with six first micro channels; the liquid outlet ends of the first micro channels communicate with first micro-ring circle channels; the liquid outletends of the first micro-ring circle channels communicate with annular channels; the annular channels are provided with twelve second micro channels; the liquid outlet ends of the second micro channels communicate with second micro-ring circle channels; the liquid outlet ends of the second micro-ring circle channels communicate with buffering channels; and the liquid outlet ends of the buffering channels communicate with cell culture chambers. The same drug treatment can be used for two different cells through the layout with different micro-pipeline networks; and therefore, the purpose of cell sorting and drug screening can be achieved by forming drug concentration gradients to act on same or different cells.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to an integrated microfluidic chip for drug screening and its application. Background technique [0002] As we all know, the microenvironment of cells in the living body is highly precise. In order to understand the instantaneous changes in the microenvironment in vivo, scholars have developed many in vitro concentration generators. It mainly includes Dunn cavity, Zigmomd cavity, biogel and other technologies. But what follows is that the above method cannot maintain a long-term concentration gradient, cannot be expanded to share a device for multiple biological types, and cannot accurately generate the required single concentration or mixed concentration. In addition, the current research on some anti-cancer drugs on cancer cells requires a lot of time and equipment, and is accompanied by huge consumption of reagents, and traditional cell culture methods cannot si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12Q1/02
Inventor 申少斐王德富张璇张芳娟刘亚君牛颜冰
Owner SHANXI AGRI UNIV
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