Application of dioscin in preparation of acute gastrointestinal injury protecting medicine
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A technology for acute gastrointestinal injury and diosgenin is applied in the application field of diosgenin in the preparation of acute gastrointestinal injury protective medicine, and can solve problems such as no diosgenin and the like.
Active Publication Date: 2016-07-06
DALIAN MEDICAL UNIVERSITY
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In addition, the patent "Application of Dioscin in the Preparation of Drugs for Protecting Kidney Damage" can only prove that dioscin can significantly down-regulate the increase of blood urea nitrogen (BUN) and urinary creatinine (SCr) caused by kidney damage, so it has kidney damage Protective effect, can be used to make kidney damage protection drugs
But so far, there is no relevant report on the application of diosgenin in the protection of acute gastrointestinal injury and in the preparation of drugs for acute gastrointestinal protection
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Embodiment 1
[0016] Example 1 Study on the therapeutic effect of diosgenin on gastric ischemia-reperfusion injury
[0017] 1. In vitro cell model establishment and grouping
[0018] The human gastric mucosal epithelial cell line (GES-1) was cultured in vitro, and the model of gastric hypoxia-reoxygenation injury (H / R) was established. , 25, 50, 100, 200, 400 and 800 ng / mL). 12 hours before cell modeling, the administration group started administration, the model group and the administration group were hypoxic for 2 hours, and after reoxygenation for 12, 24 and 48 hours respectively, the cell morphology was observed under an inverted microscope, and the cell viability was detected by MTT method.
[0019] Effects of hypoxia / reoxygenation model on GES-1 cells:
[0020] The viability and morphology of GES-1 cells after hypoxia / reoxygenation injury are shown in figure 1 , the results showed that, compared with the control group, the cell viability of the model group after reoxygenation decre...
Embodiment 2
[0035] Example 2 Study on the therapeutic effect of diosgenin on intestinal ischemia-reperfusion injury
[0036] 1. In vitro cell model establishment and grouping
[0037] Human colon adenocarcinoma cell line (Caco-2) and intestinal crypt cell line (IEC-6) were cultured in vitro, and hypoxia-reoxygenation injury (H / R) models were established respectively. Experimental groups: control group, model group, different Dosing pretreatment group (diosgenin 12.5, 25, 50, 100, 200, 400 and 800 ng / mL). The drug administration group began to administer drugs 12 hours before cell modeling, the cells in the model group and the drug administration group were hypoxic for 4 hours, and after reoxygenation for 6, 12 and 24 hours respectively, the cell morphology was observed under a microscope, and the cell viability was detected by MTT.
[0038] Effects of hypoxia-reoxygenation model on cells:
[0039] The viability and morphology of Caco-2 and IEC-6 cells after hypoxia-reoxygenation injury ...
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Abstract
The invention discloses application of dioscin which has the acute gastrointestinal injury protecting effect and can be prepared into acute gastrointestinal injury protecting medicine.An MTT method and morphological observation prove that the dioscin has the obvious protecting effect on GES-1 cells, Caco-2 cells and IEC-6 cells which are injured by in-vitro hypoxia and reoxygenation; meanwhile, the dioscin has the obvious down-regulation effect on MDA and MPO increasing caused by an acute gastrointestinal injury and has the obvious up-regulation effect on T-SOD and GSH-PX decreasing.After intervening is performed with the dioscin, the phenomena that the injury index of the ischemia reperfusion injury gastric mucosa is increased, the gastric mucosa is eroded and bleeds, and the partial gland structure is disordered are obviously improved, and the phenomena that after an ischemia reperfusion injury is generated, villus falling is obvious, the blood capillaries are expanded, gland damage is obvious, and parts of the glands bleed and are ulcerated are obviously improved.The dioscin can be prepared into the medicine by serving as a single effective ingredient and also can be prepared into a compound preparation by being cooperatively used with other medicine according to the clinical medication requirements.
Description
technical field [0001] The invention relates to a new application of active ingredients of traditional Chinese medicine, in particular to the application of high-purity diosgenin in the preparation of medicines for protecting acute gastrointestinal injury. Background technique [0002] The patent "Application of Dioscin in the Preparation of Kidney Damage-Protective Medicine" in the prior art proves that dioscin has a kidney-damage-protective effect and can be used to make kidney-damage-protective medicines; the kidney is part of the urinary system and is mainly responsible for filtering blood impurities, maintain the balance of body fluids and electrolytes, and finally produce urine to be excreted from the body through subsequent pipelines. It also has endocrine functions to regulate blood pressure; however, the gastrointestinal tract is an important digestive organ and detoxification organ of the human body. The main function of the stomach is to absorb The main function o...
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