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Bombyx mori extract and extraction method and application thereof

An extraction method and extract technology, applied in silkworm extract and its extraction method and application field, can solve the problems of patents on the purification and refining of active ingredients of silkworm, etc.

Inactive Publication Date: 2016-07-20
厦门市医药研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current literature shows that in the treatment of diabetes and other related diseases, silkworms are all used as medicine with the freeze-dried powder of whole larvae. For example, the domestic published patents CN101036671A and CN101091724A relate to the research of silkworms in the treatment of diabetes, but no relevant active ingredients of silkworms have been seen. Patents for purification and refinement

Method used

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  • Bombyx mori extract and extraction method and application thereof
  • Bombyx mori extract and extraction method and application thereof
  • Bombyx mori extract and extraction method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The preparation method of embodiment 1 silkworm methanol extract

[0023] Take a certain amount of 5th instar silkworm, freeze-dry and pulverize it to prepare silkworm freeze-dried powder, add 10-30 times of 50%-100% methanol aqueous solution, stir well, soak for more than 0.5h, ultrasonic for 10min, repeat more than 3 times, Filter each time at least 10 minutes apart; add 10 to 30 times more 50% to 100% methanol aqueous solution to the precipitate, stir thoroughly, soak for more than 0.5 hours, ultrasonic for 10 minutes, repeat more than 3 times, and filter at least 10 minutes apart each time; The filtrates were combined, concentrated under reduced pressure, freeze-dried or spray-dried to obtain the product.

Embodiment 2

[0024] The preparation method of embodiment 2 silkworm ethanol extract

[0025] Take a certain amount of 5th instar silkworm, freeze-dry and pulverize it to prepare silkworm freeze-dried powder, add 10-30 times of 50%-100% ethanol aqueous solution, stir well, soak for more than 0.5h, ultrasonic for 10min, repeat more than 3 times, Filter each time at least 10 minutes apart; add 10 to 30 times the 50% to 100% ethanol aqueous solution to the precipitate, stir thoroughly, soak for more than 0.5 hours, ultrasonic for 10 minutes, repeat more than 3 times, and filter at least 10 minutes apart each time; The filtrates were combined, concentrated under reduced pressure, freeze-dried or spray-dried to obtain the product.

Embodiment 3

[0026] Example 3 Bombyx mori alcohol extract directly enhances the effect of cell glucose uptake

[0027] Experimental materials: 3T3-L1 preadipocytes, DMEM high glucose medium, fetal bovine serum (FBS), dexamethasone (Dex), insulin (insulin), 3-isobutyl-1-methylxanthine (IBMX) , 2-deoxyglucose (2-DG), dimethyl sulfoxide (DMSO); 1-deoxynojirimycin (1-DNJ), silkworm ethanol extract

[0028] Experimental method: 3T3-L1 preadipocytes were seeded in a 12-well plate, cultured in high-glucose DMEM medium containing 10% FBS, and the medium was changed every other day; after the cells in the 12-well plate grew to full confluence for 48 hours, the The differentiation medium starts to differentiate, and the medium is changed every other day; after 72 hours of differentiation, the maintenance medium containing 100ng / ml Insulin is replaced, and the medium is changed every other day; after more than 95% of the cells are completely differentiated into mature adipocytes (day 11) , add a cer...

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Abstract

The invention relates to a bombyx mori extract, in particular to the bombyx mori extract and an extraction method and application thereof. The extraction method includes the steps that 5-old bombyx mori is taken and prepared into bombyx mori freeze-dried powder after freeze drying and smashing, after an alcohol-water solution is added, soaking, ultrasonic treatment and filtering are conducted, and filtrate A is obtained; 2, an alcohol-water solution is added to sediment obtained in the step 1, soaking, ultrasonic treatment and filtering are conducted, and filtrate B is obtained; 3, the filtrate A and the filtrate B are combined, vacuum concentration and drying are conducted, and then the bombyx mori extract is obtained. The bombyx mori extract can be applied to relieving insulin resistance and enhancing cell glucose uptake. On the aspect of relieving insulin resistance, the bombyx mori extract is applied to preparing medicine for treating or relieving insulin resistance syndromes and preparing medicine for treating type 2 diabetes, obesity, hypertension, dyslipidemia, polycystic ovarian syndromes, hyper blood-coagulation disorder, microalbuminuria and other diseases. On the aspect of enhancing cell glucose uptake, the bombyx mori extract is applied to preparing medicine for treating or relieving type 1 diabetes.

Description

technical field [0001] The invention relates to a silkworm extract, in particular to a silkworm extract and its extraction method and application. Background technique [0002] Insulin resistance refers to the decreased sensitivity and responsiveness of insulin's peripheral target tissues (mainly skeletal muscle, liver and adipose tissue) to endogenous or exogenous insulin, resulting in a physiological dose of insulin that produces a lower than normal physiological effect ( That is, insulin promotes glucose uptake and utilization efficiency decline). In the early and middle stages of insulin resistance, in order to overcome the decrease in the physiological potency of insulin, the pancreatic β cells often secrete excessive insulin compensatoryly. A series of closely related clinical symptoms caused by long-term insulin resistance and hyperinsulinemia Abnormalities such as impaired glucose tolerance or type 2 diabetes, hypertension, lipid metabolism disorder, microalbuminuri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P3/10A61P3/04A61P9/12A61P3/06A61P3/00A61P15/00A61P7/02A61P13/12A61P5/50
CPCA61K35/63
Inventor 许光辉黄亦琦罗友华杨辉
Owner 厦门市医药研究所
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