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Preparation and application of a bacterial agent of Bacillus highlander

A technology of bacillus highlandii and microbial inoculum, which is applied in the application, bactericide, bacteria and other directions to achieve the effects of simple fermentation process, significant control effect, and stable control effect

Active Publication Date: 2019-07-12
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are few reports on the control of peach postharvest diseases by Bacillus altitudinis

Method used

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  • Preparation and application of a bacterial agent of Bacillus highlander
  • Preparation and application of a bacterial agent of Bacillus highlander
  • Preparation and application of a bacterial agent of Bacillus highlander

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Isolation and screening of Bacillus highlander 14b

[0027] In the peach orchard without chemical fertilizers and pesticides, the rhizosphere soil of peach trees was collected, and the dilution separation method was used to make 10 -1 、10 -2 、10 -3 and 10-4 soil dilution, use a pipette to draw 10 -2 、10 -3 and 10 -4 Add 0.1mL of the soil dilution solution to the NA medium plate, spread it evenly with a sterile applicator, and mark the serial number. After standing for one hour, reverse the culture plate and cultivate it at 37°C. From the second day of culture, single bacterial colonies were selected according to the characteristics of the colony shape, and transplanted to NA medium respectively, and were used for subsequent experiments after 48 hours of culture. The purified strains were stored on NA slant medium at 4°C.

[0028] Antagonist bacteria were screened by plate confrontation method. Inoculate the pathogenic bacteria cake (8mm puncher) in the...

Embodiment 2

[0029] Embodiment 2: the preparation of microbial bacterial agent

[0030] The microbial bacterial agent used for preventing and treating postharvest fungal diseases of peaches includes fermentation broth and fermentation filtrate of Bacillus highlander 14b, and the preparation steps are as follows:

[0031] 1) Activated strain: transfer the preserved Bacillus uplander 14b to NA medium by streaking, and place in an incubator at 40°C for 24 hours of constant temperature and moisture cultivation;

[0032] 2) Preparation of seed solution: Pick a single colony of the antagonistic strain Bacillus upholsteria 14b that grew well on the NA medium and inoculate it into the NB liquid medium, culture it with shaking at 40°C for 48 hours, and the shaking frequency is 130-180r / min;

[0033] 3) Preparation of fermentation broth: inoculate the seed solution of strain 14b prepared in step 2) into NB liquid culture solution with an inoculum size of 3% by volume, and the charging amount is 50-1...

Embodiment 3

[0036] Embodiment 3: the influence of microbial bacterial agent on the growth of pathogenic bacteria mycelia

[0037] The microbial agent includes the fermentation liquid and the fermentation filtrate of Bacillus highlander 14b prepared in Example 2.

[0038] The filter paper method was used to measure the effect of strain 14b fermentation broth on mycelial growth. Inoculate the cakes of brown rot fungus, botrytis cinerea, soft rot fungus, and penicillium fungus in the center of the PDA plate respectively, dip a piece of sterile filter paper (Φ=8mm) into the fermentation broth of bio-control bacteria, and stick it symmetrically at a distance from the fungus cake of pathogenic bacteria Incubate at 26°C at 2.5 cm, observe and record the growth diameter of pathogenic bacteria after 3 days and calculate the inhibition rate, see Table 1 for details.

[0039]The plate method containing filtrate was used to measure the effect of fermentation broth on mycelia growth. After mixing th...

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Abstract

The invention relates to an antagonistic bacteria used for preventing and treating postharvest fungal diseases of peaches and a microbial bacterial agent prepared from the bacterial strain. The invention provides a strain of Bacillus altitudinis with a preservation number of CGMCC No. 12180 (Bacillus altitudinis), and the application of the microbial agent fermentation liquid and fermentation filtrate prepared from the Bacillus altitudinis 14b in the prevention and treatment of peach postharvest fungal diseases. The bacillus uplander 14b disclosed by the present invention is obtained by collecting the rhizosphere soil of peach trees in a peach orchard in Feicheng without applying chemical fertilizers and pesticides, and can effectively prevent peach brown rot, gray mold, soft rot and penicillium, etc. A variety of fungal diseases, it is a biological control potential strain with good biological control effect, no pollution, wide control range, safety and ecology, and has broad market application prospects.

Description

[0001] (1) Technical field [0002] The invention relates to a bacillus uplander in the field of microbes and biological control and the fermented liquid and fermented filtrate of the microbial agent prepared therefrom, in particular to its application in preventing and controlling peach post-harvest diseases. [0003] (2) Background technology [0004] Peach is an important drupe fruit tree with a wide distribution and a large planting area in my country. With the increase of cultivated area and yield year by year, brown rot caused by Monilinia fructicola, gray mold caused by Botrytis cinerea, soft rot caused by Rhizopus stolonifer Problems caused by rot and penicillium expansum caused by Penicillium expansum have become increasingly prominent, seriously affecting the storage, transportation and processing of peaches. Researchers at home and abroad are working hard to find new ways to replace or reduce chemical corrosion protection. However, the current control methods are m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 石晶盈殷晓慧王庆国张畅彭勇张欣刘佩
Owner SHANDONG AGRICULTURAL UNIVERSITY
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