Boar semen room-temperature preservation dilution powder and diluent, and application of dilution powder and diluent

A technology of storage at room temperature and diluent, which is applied in the field of artificial insemination of livestock, can solve the problems of unstable use effect, increase economic benefit, and easy agglomeration, and achieve good use effect, increase economic benefit, and low cost

Inactive Publication Date: 2016-08-17
CHONGQING ACAD OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of the existing dilution powder such as high cost, easy agglomeration, and unstable use effect, and provide a new dilution powder and dilution liquid for storing porcine semen at room

Method used

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  • Boar semen room-temperature preservation dilution powder and diluent, and application of dilution powder and diluent
  • Boar semen room-temperature preservation dilution powder and diluent, and application of dilution powder and diluent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of porcine semen normal temperature storage dilution powder and dilution

[0044] Prepare raw materials according to the dilution powder formula given in Table 2:

[0045] The dilution powder formula of table 2 embodiment 1

[0046] Reagent

[0047] sodium bicarbonate

[0048] Other operations are the same as Comparative Example 1.

Embodiment 2

[0050] Use the dilution powder described in Comparative Example 1 and Example 1 to make the dilution, and compare the preservation effect with the existing dilution powder. The semen of 5 boars was taken, and each boar was collected 2-3 times a week. The semen of a boar was divided into 4 parts, diluted with BST, BSTm dilution powder (see Table 3 for formula) and the dilution powder prepared in Comparative Example 1 and Example 1 for dilution, and stored at 17°C after subpackaging. Semen viability was checked under a 40X microscope every 12 hours.

[0051] Table 3 BST, BSTm optimized formula

[0052] Composition (g / L)

[0053] Take pig semen and boar semen, and filter it with a 300-mesh sieve, measure the semen motility and density of the filtered semen according to the method described in the national standard GB23238-2009, and calculate the dilution ratio of the original semen when the number of effective sperm is ≥ 2.5 billion. First dilute according to the ratio ...

Embodiment 3

[0060] Prepare raw materials according to the dilution powder formula given in Table 5:

[0061] The dilution powder formula of table 5 embodiment 3

[0062] Reagent

[0063] Other operations are the same as Comparative Example 1. The diluent should be prepared half an hour before the semen dilution. A pack of pig semen dilution powder is dissolved in double distilled water or ultrapure water to 1000mL, stirred continuously by a magnetic stirrer until it is completely dissolved, and the pH value is adjusted to 6.9. Heat to 37°C. According to the sperm viability and density, adjust the dilution ratio, and slowly add the diluent to the semen.

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Abstract

The invention belongs to the technical field of livestock artificial insemination, and specifically, relates to a boar semen room-temperature preservation dilution powder and diluent, and an application of the dilution powder and the diluent. The boar semen room-temperature preservation dilution powder comprises the components in parts by weight: 28-34 parts by weight of glucose, 1.5-2.5 parts by weight of sodium bicarbonate, 6.5-8.5 parts by weight of sodium citrate, 1.8-2.4 parts by weight of EDTA, 2.0-3.5 parts by weight of citric acid, 4.0-6.0 parts by weight of Tris, 3.0-5.0 parts by weight of Hepes, 1.0-2.5 parts by weight of PVP, 2.0-4.0 parts by weight of BSA, 0.05-0.10 part by weight of cysteine, and an antibiotic. The invention provides the brand-new boar semen room-temperature preservation dilution powder and diluent; an agglomeration phenomenon in the semen preservation process can be effectively reduced, the semen preservation time is prolonged, and the utilization efficiency of excellent breeding boars is expanded. Boar semen is diluted and subpacked by the whole-component diluent prepared by the formula and is preserved at the temperature of 17 DEG C, 3 days later, the semen vigor is 0.6 or more, and the pig breeding and production needs can be met.

Description

technical field [0001] The invention belongs to the technical field of artificial insemination of livestock, and in particular relates to a dilution powder for storing porcine semen at room temperature, a dilution solution and applications thereof. Background technique [0002] With the continuous expansion of the pig breeding scale, pig artificial insemination technology has been widely implemented in the pig industry, and the construction of a large number of professional socialized boar studs has put forward higher requirements for the preservation of normal temperature semen. In order to improve the utilization rate of breeding boars and the work efficiency of artificial insemination of pigs, many countries use the pig semen diluted with diluent to store them at room temperature and then carry out insemination operations. It is generally believed that semen dilution powder should meet the following characteristics: 1) non-invasive effect on sperm; 2) can supply sperm nut...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张亮王涛邱进杰
Owner CHONGQING ACAD OF ANIMAL SCI
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