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Application of Biflavonoids of Spinyflower in the Preparation of Drugs for Treating Thrombocytopenia

A kind of technology of amiflavonoids and thrombocytopenia, which is applied in drug combination, blood diseases, pharmaceutical formulas, etc., can solve the problems of unreported treatment of amiflavonoids of A. Inhibition of thrombocytopenic purpura, inhibition of thrombocytopenia, less toxic and side effects

Active Publication Date: 2018-04-13
NANJING HAOCHUANG PHARMA TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the above-mentioned prior art does not report the use of abiflavones in the treatment of thrombocytopenia, nor disclose the application of abiflavones in the treatment of thrombocytopenia

Method used

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  • Application of Biflavonoids of Spinyflower in the Preparation of Drugs for Treating Thrombocytopenia
  • Application of Biflavonoids of Spinyflower in the Preparation of Drugs for Treating Thrombocytopenia
  • Application of Biflavonoids of Spinyflower in the Preparation of Drugs for Treating Thrombocytopenia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 In vitro cell activity screening of Selaginella flavonoids

[0035] 1.1 Test materials and instruments

[0036] 1.1.1 Test material

[0037] Dami cells, Guangzhou Geneo Biotechnology Co., Ltd.; RPMI-1640 medium, Nanjing Kage Biotechnology Development Co., Ltd.; fetal bovine serum, ScienCell, USA; double antibodies (penicillin, streptomycin) Nanjing Kage Biotechnology Co., Ltd. Company; trypan blue, Canada BioBasic Co., Ltd.; methyl thiazolyl tetrazolium (MTT), Nanjing Kaiji Biotechnology Development Co., Ltd.; β-mercaptoethanol, American Amresco; Giemsa (Giemsa) pigment, Sinopharm Group Chemical Reagent Co., Ltd.; agar powder, PeproTech, USA; human thrombopoietin (humanthrombopoietin, TPO), PeproTech, USA; CD41-FITC, BioLegend, USA; propidium iodide (PI) dye, RNase, USA Sigma company; Biflavones of Spiny chinensis, Torreya biflavones, Podocarpus biflavones A, Cypress biflavones, etc. are all isolated from Selaginella chinensis (S.Mollendorfii), and their str...

Embodiment 2

[0071] Example 2 Effects of Amiflavones of Spinyflower on Injection of Carboplatin-Induced BALB / c Mice Thrombocytopenia Model

[0072] 2.1 Test materials and instruments

[0073] 2.1.1 Test drug and preparation method:

[0074] Carboplatin injection, specification 100mg: 10ml, Qilu Pharmaceutical Co., Ltd., batch number: 9080082ES, made into 6.25mg / ml with normal saline before use.

[0075]Recombinant human interleukin-11 (IL-11) for injection, specification 100mg: 10ml, Qilu Pharmaceutical Co., Ltd., batch number: 201412061SM, made into 0.05mg / ml with normal saline before use.

[0076] The test drug, abiflavone of Suicha, is self-made in the laboratory. Before use, it is formulated with normal saline at 0.5, 1.5, and 5 mg / ml for intragastric administration.

[0077] 2.1.2 Test equipment:

[0078] Blood counting plate, Shanghai Qiujing Biochemical Reagent Co., Ltd.; OLYMPUS-CH ordinary optical microscope, Japan OLYMPUS company.

[0079] 2.1.3 Test animals:

[0080] Clean ...

Embodiment 3

[0108] Example 3 Effect of Biflavonoids of Spiny Flowers on Idiopathic Thrombocytopenic Purpura

[0109] 3.1 Test materials and instruments

[0110] 3.1.1 Test drug and preparation method:

[0111] Prednisone acetate tablets, specification: 5mg / tablet, Tianjin Pharmaceutical Group Xinzheng Co., Ltd., batch number: 150126, grind into powder before use, and make 1mg / ml suspension with normal saline. The test drug, abiflavone of Suicha, is self-made in the laboratory. Before use, it is formulated with normal saline at 0.5, 1.5, and 5 mg / ml for intragastric administration.

[0112] 3.1.2 Test equipment:

[0113] Blood counting plate, Shanghai Qiujing Biochemical Reagent Co., Ltd.; OLYMPUS-CH ordinary optical microscope, Japan OLYMPUS company.

[0114] 3.1.3 Test animals:

[0115] Clean grade BALB / C mice, male, weighing 18-22g, Yangzhou University Comparative Medicine Center, certificate number: SCXK (Su) 2012-0004; healthy normal guinea pigs, weighing 250-350g, Qinglongshan An...

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PUM

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Abstract

The invention relates to a purpose of medicine for treating thrombocytopenia, in particular to application of amentoflavone to the preparation of medicine for treating thrombocytopenia. The amentoflavone can be prepared into an oral preparation or an injection preparation by adding pharmaceutically acceptable conventional auxiliary materials. Pharmacodynamic experiments show that the amentoflavone can effectively inhibit the thrombocytopenia caused by chemotherapeutics medicine and can be used for treating thrombopenic purpura; the curative effect is obvious; and the toxic and side effects are low.

Description

technical field [0001] The invention belongs to the technical field of medicines, and relates to a medicine for treating thrombocytopenia, in particular to the application of the biflavone of spicantha fir in the preparation of medicine for treating thrombocytopenia. Background technique [0002] Platelets are biologically active small pieces of cytoplasm that are shed from the cytoplasm of mature megakaryocytes in the bone marrow. Platelets have a specific morphological structure and biochemical composition, and have a relatively constant number in normal blood. The number of platelets in healthy people is 100×10 9 / L~300×10 9 / L. Platelets play an important role in physiological and pathological processes such as hemostasis, wound healing, inflammatory response, thrombosis, and organ transplant rejection. [0003] Normal peripheral platelet count <100×10 9 / L is called thrombocytopenia. Bleeding due to thrombocytopenia accounts for approximately 30% of clinical ble...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61P7/04A61P7/06A61P7/00
CPCA61K31/352
Inventor 汪豪熊非
Owner NANJING HAOCHUANG PHARMA TECH CO LTD
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