SSR molecular marker for salt tolerance of Fraxinus velutina
A technology of molecular markers, villi wax, applied in the field of molecular biology
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[0031] 1. Extraction of Genomic DNA from A. tomentosa
[0032] (1) Material
[0033] Salt-tolerant velvet wax H (Fraxinus velutina Torr) and salt-sensitive velvet wax S. Take the fresh leaves that have just grown, seal them with a fresh-keeping bag, put them in an ice box, and bring them back to the Shandong Academy of Forestry Sciences, freeze them in liquid nitrogen, and store them in a -70°C refrigerator for later use.
[0034] (2) Using the CTAB method to extract genomic DNA from leaves of A. tomentosa
[0035] 1) Preheat the prepared CTAB extraction buffer solution in a water bath at 65°C for 30 minutes, and place chloroform:isoamyl alcohol (24:1), absolute ethanol, and 70% ethanol in a -20°C refrigerator for pre-cooling. The mortar needs to be pre-cooled in advance, and then pre-cooled with a small amount of liquid nitrogen before use.
[0036] 2) Take 0.1-0.2g of white wax young leaves, add sufficient liquid nitrogen (add in two times) and grind to powder, quickly tr...
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