Pseudomonas alternata with the function of degrading polycyclic aromatic hydrocarbons at low temperature and its application

A technology of Pseudomonas alternata and polycyclic aromatic hydrocarbons, applied in the field of microorganisms, can solve problems such as lack of research results, and achieve the effect of simple cultivation method and strong naphthalene degradation ability

Active Publication Date: 2019-09-06
DALIAN MARITIME UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are few studies on low-temperature degradation bacteria of marine petroleum hydrocarbons in my country, especially the research results on low-temperature degradation bacteria of marine PAHs are even scarcer.

Method used

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  • Pseudomonas alternata with the function of degrading polycyclic aromatic hydrocarbons at low temperature and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Isolation and identification of bacterial strain Pseudoalteromonas sp.QN-1

[0020] Isolation of Strain Pseudoalteromonas sp.QN-1

[0021] (1) Sample collection: Jiaozhou Bay;

[0022] (2) Enrichment and acclimatization: Add 100 mL of MMC culture solution into a 250 mL Erlenmeyer flask, and sterilize at 120° C. for 15 min. Dichloromethane was sterilized by filtration with a 0.22 μm pore size organic solvent-resistant filter membrane. Weigh 0.2g of naphthalene and dissolve it in 20mL of sterilized dichloromethane, then transfer it to 100mL of sterilized MMC culture medium, and shake it on a shaker overnight to volatilize the dichloromethane. After dichloromethane volatilizes, add 5 mL of seawater sample, place it in a low-temperature biochemical incubator, shake and cultivate at 120 r / min, 10 ° C for 30 days, and then take 5 mL of culture solution from it and transfer again: add 5 mL of culture solution to the solution containing 0.2 g Naphthalene in 100mL M...

Embodiment 2

[0027] Fermentation of Example 2 bacterial strain Pseudoalteromonas sp.QN-1

[0028] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100mL of sterilized 2216E liquid medium, and culture it under low-temperature shaking for 7 days (0°C, 120r / min) to become a seed solution; The liquid was added into the 2216E liquid medium at a volume ratio of 10%, and cultured at 0° C. for 7 days to obtain a fermentation liquid.

Embodiment 3

[0029] Example 3 Determination of Naphthalene Degradation Rate of Bacterial Strain Pseudoalteromonas sp.QN-1 at 0°C

[0030] (1) Degrading bacteria seed solution

[0031] Use a sterile inoculation loop to pick up the purified bacterial lawn into a conical flask filled with 100 mL of sterilized 2216E liquid medium, and culture it with shaking at low temperature for 7 days (0°C, 120r / min).

[0032] (2) Degradation culture

[0033] Weigh 0.2g of naphthalene and dissolve it in 20mL of filter-sterilized n-hexane, transfer it to a conical flask filled with 100mL of sterilized MMC liquid medium, and place it on a shaker until the n-hexane volatilizes completely. 10% of the inoculation amount was used to inoculate the seed solution of the degrading bacteria, and the culture was shaken at low temperature for 30 days (0° C., 120 r / min). The culture medium not inoculated with degrading bacteria was used as the blank group.

[0034] (3) Extraction

[0035]After the degradation culture...

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Abstract

The invention discloses a strain of Pseudoalteromonas sp. QN‑1 capable of degrading petroleum at low temperature, and its preservation number is CGMCC No. 11635. The strain was selected from the oil-polluted sea area in the north of my country, and it can degrade oil at low temperature, especially naphthalene in oil at an extreme environment of 0°C. The Pseudomonas alternata QN-1 of the present invention can be applied to the bioremediation work of marine oil pollution, especially can be applied to the degradation of naphthalene in low temperature environments such as winter (including ice age) in the northern sea area of ​​my country.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a marine low-temperature bacterium and its application in bioremediation of marine naphthalene pollution. The strain can degrade petroleum hydrocarbons at a low temperature of 0°C to 15°C, especially has the characteristics of efficiently degrading naphthalene in petroleum hydrocarbons under extreme conditions of 0°C, and can be used for biological treatment of oil spill pollution and naphthalene pollution in northern seas of my country. repair work. Background technique [0002] With the rapid development of the marine transportation industry and the rapid rise of the marine mining industry, marine oil spill accidents have become increasingly frequent and harmful. In particular, oil spills in low-temperature seas cause organic pollutants to persist due to the low temperature, which will cause greater harm to the local ecosystem. Polycyclic aromatic hydrocarbons (PAHs) in petrole...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/32C02F103/08
CPCC02F3/344C02F2101/327C02F2103/08C12N1/205C12R2001/01
Inventor 郭平林建国曹宾霞
Owner DALIAN MARITIME UNIVERSITY
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