Method for detecting citrinin in monascus pigment powder by solid-phase extraction-liquid chromatography mass spectrometry
A technology of monascus pigment and citrinin, which is applied in the detection field of solid phase extraction-liquid mass tandem detection of citrinin and citrinin in monascus pigment powder
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[0022] 1 material
[0023] 1.1 Instrument
[0024] Agilent1290-6490 triple quadrupole liquid chromatography-mass spectrometer (Agilent, USA), HLB-SPE column (60mg / 3ml, Shanghai Anpu Scientific Instrument Co., Ltd.), Agilent ZORBAX Eclipse Plus (2.1×50mm, 1.8μm; Agilent, USA ), XA205DU electronic balance (Mettler Toledo, Germany), ultrasonic cleaner.
[0025] 1.2 Monascus pigment powder and reagents
[0026] Two batches of red yeast rice pigment powder were obtained from market samples.
[0027] Citrinin (batch number: 518-75-2-150403, the standard product was purchased from Pribolab, purity > 98%), methanol and acetonitrile were chromatographically pure, other reagents were analytically pure, and the experimental water was purified by the ELGA PURELAB ClassicUVF purification system Filtered deionized water.
[0028] The percentage concentration involved in the present invention, unless otherwise specified, refers to the volume concentration of the liquid, and refers to the...
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