Kit for determining pepsinogen II and preparation method thereof

A pepsinogen and kit technology, applied in the fields of medicine and biochemistry, can solve problems such as complicated operation and low measurement accuracy, and achieve the effects of convenient operation, improved accuracy, and increased suspension stability

Inactive Publication Date: 2016-08-31
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kit for measuring pepsinogen II and its preparation method in order to overcome the defects of complicated operation and low measurement accuracy in the prior art

Method used

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  • Kit for determining pepsinogen II and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0063] Reagent R1:

[0064] MES buffer 100 mmol / L

[0065] Potassium sulfate 200 mmol / L

[0066] Polyethylene glycol-2000 35 g / L

[0067] Sodium azide 1.0 g / L

[0068] The solvent is purified water

[0069] Reagent R2:

[0070] MES buffer 35mmol / L

[0071] Sucrose 30g / L

[0072] Sodium azide 1.0 g / L

[0073] Latex-coated anti-human pepsinogen II antibody 10 g / L

[0074] Its solvent is purified water.

Embodiment 2

[0076] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0077] Reagent R1:

[0078] Tris buffer 180 mmol / L

[0079] Sodium chloride 105 mmol / L

[0080] Polyethylene glycol-4000 20 g / L

[0081] Sodium azide 1.5 g / L

[0082] The solvent is purified water

[0083] Reagent R2:

[0084] Tris buffer 10 mmol / L

[0085] Tween-20 45 g / L

[0086] Sodium azide 1.5 g / L

[0087] Latex-coated anti-human pepsinogen II antibody 16 g / L

[0088] Its solvent is purified water.

Embodiment 3

[0090] Kit preparation and method of use

[0091] 1. Preparation of latex-coated anti-human pepsinogen II antibody: Dilute polystyrene microspheres with a particle size of 120 nm with 50 mmol / L MES buffer solution to obtain a mass concentration of polystyrene microspheres of 4 % solution, and then add 0.5 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride to each milliliter of the solution, react for 1 hour at 28°C, and use a centrifuge Machine, centrifuge at 25000 rpm / min for 30 minutes, remove the supernatant, then suspend the precipitate in 50 mmol / L MES buffer, use an ultrasonic disperser for ultrasonic dispersion, and use a centrifuge at 25000 rpm / min Centrifuge for 30 minutes, remove the supernatant, suspend the pellet in 50 mmol / L MES buffer, disperse by ultrasonic, then add an equal volume of MES buffer containing 8 mg / mL anti-human pepsinogen II antibody while stirring , mixed and stirred, reacted at room temperature for 2 hours, so that the final mas...

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Abstract

The invention discloses a kit for determining pepsinogen II and a preparation method thereof. The kit comprises double liquid components including a reagent R1 and a reagent R2 which are independent from each other, wherein the reagent R1 is prepared from a buffering solution, inorganic salt, an accelerant and a preservative; and the reagent R2 is prepared from a buffering solution, a stabilizer, a preservative and an emulsion-coated anti-human pepsinogen II antibody. The preparation method comprises the following steps: preparing the reagents according to the content of the components; mixing a sample to be detected with the reagent R1 and the reagent R2 to achieve sufficient reaction; determining a reacted absorbance difference value by utilizing a full-automatic biochemical analyzer; and calculating the concentration of the pepsinogen A in a sample according to an absorbance change value. The kit gas has the advantages of high detection accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring pepsinogen II and a preparation method thereof. Background technique [0002] Pepsinogen is synthesized by the main cells of the oxyntic gland, and becomes pepsin under the action of hydrochloric acid or active pepsin in the gastric cavity. Pepsinogen is the precursor of pepsin. According to its biochemical properties and immunogenicity, it will It is divided into two subgroups, components 1-5 have the same immunogenicity, called pepsinogen Ⅰ, mainly secreted by the principal cells and mucus neck cells of the fundic gland, and components 6 and 7 are called pepsinogen II, in addition to being secreted by the principal cells and mucus neck cells of the fundic glands, the mucus neck cells of the pyloric glands of the cardia glands and gastric antrum and the upper duodenum can also produce pepsinogen II, so the concentration of pepsinogen in ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/546
CPCG01N33/573G01N33/54313G01N2333/96477
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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