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Method for detecting citrinin in red yeast rice pigment powder

A detection method, the technology of monascus pigment, is applied in the field of chemical detection to achieve high-sensitivity analysis and reduce the use of toxic reagents

Inactive Publication Date: 2016-09-07
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, the Chinese Pharmacopoeia has not yet used citrinin as a control indicator for Chinese patent medicines containing red yeast rice

Method used

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  • Method for detecting citrinin in red yeast rice pigment powder
  • Method for detecting citrinin in red yeast rice pigment powder
  • Method for detecting citrinin in red yeast rice pigment powder

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Experimental program
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Effect test

Embodiment 1

[0018] 1 material

[0019] 1.1 Instrument

[0020] Agilent1295-6550C triple quadrupole liquid chromatography mass spectrometer (Agilent, USA), HLB-SPE column (60mg / 3ml, Shanghai Anpu Scientific Instrument Co., Ltd.), Agilent ZORBAX Eclipse Plus (2.1×50mm, 1.8μm; Agilent, USA) , XA205DU electronic balance (Mettler Toledo, Germany), ultrasonic cleaner.

[0021] 1.2 National Monascus Pigment Powder and Reagents

[0022] There are 4 batches of Chinese red yeast rice pigment powder, all of which come from market sales samples.

[0023] Citrinin (batch number: 518-75-2-150403, standard product purchased from Pribolab, purity > 98%), ethanol and methanol are chromatographically pure, other reagents are analytically pure, experimental water is purified by ELGA PURELAB ClassicUVF system Filtered deionized water.

[0024] The percentage concentration involved in the present invention, unless otherwise specified, refers to the volume concentration of the liquid, and refers to the mas...

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Abstract

The invention provides a method for detecting citrinin in red yeast rice pigment powder, and belongs to the technical field of chemical detection. The detection method has the advantages of short detection time and high sensitivity. The detection method comprises the following steps: (1) preparing a standard solution; (2) adding 0.5 gram of red yeast rice pigment powder into a 10 mL conical flask, adding 2 mL of formic acid-methanol solution (1%), carrying out ultrasonic extraction (350 W) for 10 minutes at a temperature of 50 DEG C, cooling, filtering, transferring the filtrate to a 25 mL volumetric flask, adding water to the scale, evenly shaking, filtering, making the filtrate go through a small C18 solid phase extraction column at a flowing speed of 1 mL / min, washing the small column by 5 mL of water, discarding, then washing the small column by 5 mL of ethanol, collecting the eluent, blowing the eluent by nitrogen gas at a temperature of 40 DEG C to dry the eluent, precisely adding 1 mL of ethanol to dissolve the obtained solids, filtering the solution by an organic filter membrane with a thickness of 0.22 [mu]m to obtain a sample to be detected, loading the sample on an Agilent 1295-6550C triple quadrupole HPLC-MS, and analyzing the sample.

Description

technical field [0001] The invention provides a method for detecting citrinin, in particular to a method for detecting citrinin in monascus pigment powder; it belongs to the technical field of chemical detection. Background technique [0002] Citrinin was first isolated from Penicillium citrinum in 1931. Its molecular formula is C 13 h 14 o 5 , the molecular weight is 250.25. Citrinin has certain nephrotoxicity and hepatotoxicity, can cause teratogenicity, cause tumor and induce mutation. Red yeast rice is a traditional Chinese medicinal material, which is cultivated and fermented in rice with a unique monascus mold. Because some Monascus strains can produce statins that inhibit cholesterol biosynthesis, the traditional Chinese medicine Monascus has been developed as a Chinese Monascus pigment powder and health food for lowering blood lipids. In 1995, after the French scholar Blanc et al. first discovered that citrinin was contained in red yeast rice products, citrinin...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 陈学松罗达龙王华
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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