A kind of method that promotes the fermentation growth of butyric acid bacteria
A technology of butyric acid bacteria and fermentation tank, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., to achieve the effect of clear ingredients, low use concentration, and alleviating the symptoms of vitiligo
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] (1) Preparation of medium
[0022] Glucose 20 g / L, peptone 30 g / L, yeast powder 5 g / L, dipotassium hydrogen phosphate 0.5 g / L, magnesium sulfate heptahydrate 0.5 g / L, light calcium carbonate 1 g / L, manganese sulfate monohydrate 0.02 g / L, pH7.3; anaerobic tube slant medium needs to be added with agar, the agar concentration is 20 g / L; pH 7.3. After deoxygenation, it was sterilized by steam at 0.1 MPa for 20 minutes for later use.
[0023] (2) Seed expansion culture and collection of bottled seed cells
[0024] According to the requirements of aseptic and anaerobic operation, the cells were transferred from the freeze-dried tube of Butyricum strain to the newly prepared slant medium of anaerobic tube. After incubating at 37°C for 12 h, according to the requirements of sterile and anaerobic operation, add 10 mL sterile and anaerobic water to prepare anaerobic tube seed solution.
[0025] 120 mL of anaerobic bottled culture medium 60 mL, in the proportion of 1% (v / v) But...
Embodiment 2
[0030] (1) Preparation of culture medium: Same as Example 1.
[0031] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: Same as in Example 1.
[0032] (3) Fermentation culture: Except for the addition of regulatory factors, the rest of the fermentation conditions are the same as in Example 1.
[0033] Regulatory factor supplementation: Aseptic and anaerobic uniform flow of tyrosine solution (concentration: 2.5 g / L) was added within 1 hour after the start of fermentation, so that the total concentration of tyrosine was 0.0125 g / L.
[0034] Anaerobic fermentation was carried out for 14 h as above, and the number of cells (counted by colony CFU by Hungate rolling tube counting method) was 5.52×10 8 CFU / mL, increased by 49.2% compared with Example 1; the acetic acid produced by every 100 million cells in the fermentation broth was 0.13 mg, which was 31.6% lower than that of Example 1; A decrease of 27.7%.
Embodiment 3
[0036] (1) Preparation of culture medium: Same as Example 1.
[0037] (2) Expanded cultivation of seed solution and collection of seed cells in bottle: Same as in Example 1.
[0038] (3) Fermentation culture: Except for the addition of regulatory factors, the rest of the fermentation conditions are the same as in Example 1.
[0039] Regulatory factor addition: Aseptic and anaerobic feeding of tyrosine solution (concentration: 2.5 g / L) within 1 hour of the start of fermentation, so that the total concentration of tyrosine is 0.025 g / L.
[0040]Anaerobic fermentation was carried out for 14 h as above, and the number of cells (counted by colony CFU by Hungate rolling tube counting method) was 6.56×10 8 CFU / mL, increased by 77.3% compared with Example 1; the acetic acid produced by every 100 million cells in the fermentation broth was 0.1 mg, which was 47.4% lower than that of Example 1; A decrease of 41.8%.
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More