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Immunological competence improving acid degradation product of polysaccharides of stems and leaves of abelmoschus esculentus and method for preparing immunological competence improving acid degradation product

A degradation product, the technology of hollyhock, which is applied in the direction of organic active ingredients, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of less activity research, and achieve the protection significance of improving immune activity and good ecological environment. The effect of economic value

Inactive Publication Date: 2016-09-28
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, domestic and foreign studies on polysaccharides from stems and leaves of hollyhock mainly focus on the analysis of physical and chemical properties and monosaccharides, and there are few studies on its activity.

Method used

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  • Immunological competence improving acid degradation product of polysaccharides of stems and leaves of abelmoschus esculentus and method for preparing immunological competence improving acid degradation product
  • Immunological competence improving acid degradation product of polysaccharides of stems and leaves of abelmoschus esculentus and method for preparing immunological competence improving acid degradation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 The preparation method of the acid degradation product of hollyhock stem and leaf polysaccharide comprises the following steps:

[0028] (1) Preparation of crude polysaccharides from stems and leaves of hollyhock

[0029] Take 100 g of hollyhock stem and leaf coarse powder, add 30 times the amount of water, and extract under reflux in a water bath at 100°C for 3 times, each time for 1 hour. Filter, combine the filtrates, and concentrate under reduced pressure. Remove protein by Sevag method, centrifuge (4000r / min, 10min), take the supernatant, add four times the volume of absolute ethanol, alcohol precipitation overnight, filter with suction, wash the precipitate three times with absolute ethanol, acetone, ether, 50°C drying to obtain the crude polysaccharide from the stems and leaves of the hollyhock.

[0030] (2) Fractionation of crude polysaccharides from stems and leaves of hollyhock

[0031] Weigh 3 g of crude polysaccharide, add an appropriate amount ...

Embodiment 2

[0034] Example 2 Determination of total sugar and uronic acid content of polysaccharides from stems and leaves of hollyhock and its acid degradation products

[0035] 1. Experimental method: with D-anhydrous glucose as the standard, the total sugar content of polysaccharides in the stems and leaves of hollyhock before and after sulfation modification was determined by the phenol-sulfuric acid method; Aldehydic acid is the standard.

[0036] 2. Experimental results: the total sugar content of hollyhock stem and leaf polysaccharides is 99.76%, and the total sugar content of degradation products has decreased to 80.68%; the hollyhock stem and leaf polysaccharides do not contain uronic acid before degradation, and do not contain uronic acid after degradation acid, which is consistent with the results for monosaccharide composition.

Embodiment 3

[0037] Example 3 Composition analysis of polysaccharides from stems and leaves of hollyhock and its monosaccharides from acid degradation products

[0038]1. Experimental method: use trifluoroacetic acid to hydrolyze polysaccharide samples, use mixed monosaccharides as standard products, PMP derived polysaccharide samples and mixed standards, use high performance liquid chromatography to determine the composition of monosaccharides according to the peak time, and draw each according to the peak area. The monosaccharide standard curve was used to determine the monosaccharide content of the sample.

[0039] 2. Experimental results: analysis of monosaccharide composition, as shown in Table 1:

[0040] Table 1 Molar ratio of monosaccharide composition of polysaccharides in stems and leaves of hollyhock and its acid degradation products

[0041]

[0042] The molar ratios of polysaccharides in the stems and leaves of hollyhock and the monosaccharides of the acid hydrolysis produ...

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PUM

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Abstract

The invention discloses an immunological competence improving acid degradation product of polysaccharides of stems and leaves of abelmoschus esculentus and a method for preparing the immunological competence improving acid degradation product. The acid degradation product of the polysaccharides of the stems and leaves of the abelmoschus esculentus comprises mannose, glucose, galactose and arabinose. A molar ratio of the mannose to the glucose to the galactose to the arabinose is 0.33:17.03:1.00:0.41. The molecular weight of the acid degradation product of the polysaccharides of the stems and leaves of the abelmoschus esculentus is 715.17 kDa. The method includes optimizing extraction and separation technologies via large quantities of experiments; acquiring crude polysaccharides by the aid of water extraction and alcohol precipitation processes; removing protein; purifying the crude polysaccharides by the aid of DEAE-52 cellulose resin to obtain the high-purity polysaccharides of the stems and leaves of the abelmoschus esculentus; acquiring the acid degradation product of the polysaccharides of the stems and leaves of the abelmoschus esculentus by means of acid hydrolysis. The immunological competence improving acid degradation product and the method have the advantages that waste stem and leaf resources of the abelmoschus esculentus can be sufficiently utilized, accordingly, waste can be turned into wealth, the immunological competence improving acid degradation product of the polysaccharides of the stems and leaves of the abelmoschus esculentus can be prepared, traditional Chinese medicine resources can be sustainably applied, and the immunological competence improving acid degradation product and the method have excellent economic value.

Description

technical field [0001] The invention relates to a plant polysaccharide, in particular to an acid degradation product of hollyhock stem and leaf polysaccharide capable of improving immune activity and a preparation method thereof. Background technique [0002] Traditional Chinese medicine resources are a solid foundation for safeguarding national health and developing ethnic medicine. In recent years, the production area of ​​traditional Chinese medicine and natural medicinal biological resources has exceeded 2.40×10 6 hm 2 , the output of medicinal materials can reach 5.40×10 6 t, while the biomass of discarded plant roots and aboveground stems and leaves is as high as 1.1×10 7 ~1.6×10 7 t is 2 to 3 times the output of medicinal materials, causing serious waste of resources and environmental pollution. Therefore, in the process of industrialization of traditional Chinese medicine resources, improving the utilization value of medicinal biological resources, especially en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04A61K36/185
CPCA61K31/715A61K36/185C08B37/0003C08B37/006
Inventor 江曙潘欣欣段金廒朱悦钱大玮严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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