Method for preparing cellular material from biological cross-linking agent crosslinked protein

A technology of biological cross-linking agent and cross-linking protein, which is applied in the field of preparation of porous materials by cross-linking protein with biological cross-linking agent, can solve the problems of inapplicability and limited application range, and achieve low raw material, wide application range and simple preparation process Effect

Inactive Publication Date: 2016-09-28
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing literature (Nadeem Siddiqui, Esmaiel Jabbari, et al.Osteogenic differentiation of human mesenchymalstem cells in freeze-gelled chitosan / nanoβ-tricalcium phosphate porous scaffolds crosslinkedwith genipin[J].Materials Science and Engineering:C.2019-54: 83) Using β-tricalcium phosphate and chitosan as raw materials, genipin as a cross-linking agent to prepare porous materials for human bone scaffold materials, which can only be used for medical bone scaffold materials and cannot be used in food biology and other fields, limited range of applications

Method used

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  • Method for preparing cellular material from biological cross-linking agent crosslinked protein
  • Method for preparing cellular material from biological cross-linking agent crosslinked protein
  • Method for preparing cellular material from biological cross-linking agent crosslinked protein

Examples

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Embodiment 1

[0028] A method for preparing a porous material with a biological cross-linking agent cross-linked protein, comprising the following steps:

[0029] (1) Prepare an acetic acid solution with a mass concentration of acetic acid of 1%, and add chitosan equivalent to 0.05% of the mass of the acetic acid solution into the acetic acid solution to fully hydrate it.

[0030] (2) An ethanol solution with a mass concentration of ethanol of 70% was prepared, and wheat prolamin equivalent to 2.5% of the mass of the ethanol solution was added into the ethanol solution to fully dissolve it.

[0031] (3) Get the material obtained in step (2) that is equivalent to 40% of the volume of the material obtained in step (1) and add it to the material in step (1), and shear and homogenize under the rotating speed of 6000r / min.

[0032] (4) The material obtained in step (3) is rotatably evaporated in a water bath until the mass concentration of wheat prolamin composite particles is 2%.

[0033] (5) ...

Embodiment 2

[0046] A method for preparing a porous material with a biological cross-linking agent cross-linked protein, comprising the following steps:

[0047] (1) Prepare an acetic acid solution with a mass concentration of acetic acid of 1%, and add chitosan equivalent to 0.05% of the mass of the acetic acid solution into the acetic acid solution to fully hydrate it.

[0048] (2) An ethanol solution with a mass concentration of ethanol of 70% was prepared, and wheat prolamin equivalent to 2.5% of the mass of the ethanol solution was added into the ethanol solution to fully dissolve it.

[0049] (3) Get the material obtained in step (2) that is equivalent to 40% of the volume of the material obtained in step (1) and add it to the material in step (1), and shear and homogenize under the rotating speed of 6000r / min.

[0050] (4) The material obtained in step (3) is rotatably evaporated in a water bath until the mass concentration of wheat prolamin composite particles is 2%.

[0051] (5) ...

Embodiment 3

[0059] A method for preparing a porous material with a biological cross-linking agent cross-linked protein, comprising the following steps:

[0060] (1) Prepare an acetic acid solution with a mass concentration of acetic acid of 1%, and add chitosan equivalent to 0.05% of the mass of the acetic acid solution into the acetic acid solution to fully hydrate it.

[0061] (2) An ethanol solution with a mass concentration of ethanol of 70% was prepared, and wheat prolamin equivalent to 2.5% of the mass of the ethanol solution was added into the ethanol solution to fully dissolve it.

[0062] (3) Get the material obtained in step (2) that is equivalent to 40% of the volume of the material obtained in step (1) and add it to the material in step (1), and shear and homogenize under the rotating speed of 6000r / min.

[0063] (4) The material obtained in step (3) is rotatably evaporated in a water bath until the mass concentration of wheat prolamin composite particles is 2%.

[0064] (5) ...

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Abstract

The invention discloses a method for preparing a cellular material from biological cross-linking agent crosslinked protein. The method comprises the following steps that 1, chitosan is added into an acetic acid solution and is fully hydrated; 2, wheat gliadin is added into an ethanol solution to be fully dissolved; 3, the material obtained in the step 1 and the material obtained in the step 2 are mixed, shear-homogenized, centrifuged and rotationally evaporated through an anti-solvent method, and wheat gliadin compound colloidal particles are obtained; 4, genipin is added into the material obtained in the step 3 to be fully dissolved; 5, normal hexane is added into the material obtained in the step 4 for shearing and homogenizing, and the novel cellular material is prepared after freeze drying. The obtained cellular material is rich in raw material source, low in cost and high in porosity and specific surface area, has certain anti-pressure ability, and has certain prospects in the fields of biological catalysis, chemical industry, medicine, food and the like.

Description

technical field [0001] The invention relates to a novel porous material, in particular to a method for preparing the porous material by cross-linking protein with a biological cross-linking agent. technical background [0002] The research on porous materials has become a major research hotspot in the field of materials. Porous materials have many special properties, such as high porosity, high specific surface area, high adsorption, high screening, etc., which other materials do not have. The properties make porous materials widely used in chemical industry, functional materials, medicine, environmental protection, food and many other fields. [0003] The raw materials used in the currently developed porous materials are basically metals, ceramics, organic and inorganic chemical reagents, etc., and their common feature is that they are inedible raw materials or raw materials that have certain toxic effects on the human body. In addition, the cross-linking agents used in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L89/00C08L5/08C08J3/24C08J9/14
CPCC08J3/246C08J9/141C08L89/00C08J2203/14C08J2389/00C08J2405/08C08L2201/06C08L5/08
Inventor 尹寿伟曾涛杨晓泉
Owner SOUTH CHINA UNIV OF TECH
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