Plant tissue culture support

A plant tissue culture and support technology, applied in plant regeneration, botany equipment and methods, horticulture, etc., can solve the problems of poor growth, poor fixation of explants, etc., to reduce pollution and reduce tissue culture costs , time-saving effect

Inactive Publication Date: 2016-10-12
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Absorbent cotton is easy to absorb water and has good air permeability, and the growth of callus in the medium used as a support is better. It is also because of the good water absorption of absorbent cotton that often leads to insufficient culture medium and poor growth of explants in the later stage; When the polyurethane sponge is used as the support, the explants grow vigorously, and the cost is low, saving labor and labor. The roots of the cultures can be directly moved into the shade without washing with water, so the survival rate is high, but there are also problems in the later stage due to the strong absorption capacity. Insufficient nutrient solution affects the growth of explants, and it is found that vitreous seedlings are produced; vermiculite and perlite as supports have the characteristics of low cost, simple operation, and good growth of explants, but the disadvantage is that the explants The fixing effect is not very good, which is the same as glass balls, glass wool, quartz sand, loam, river sand, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Select the leaf buds of the dicotyledonous vine rose, wash them with running water, peel off the scales outside the buds, sterilize the buds in 1:1 (W / V) garlic aqueous solution for 30 minutes, and inoculate them immediately on the last leaves of the vine rose. Suitable primary culture medium (MS+6-BA0.5mg / L+NAA0.05mg / L+3% sucrose). After 30 days, the germinated leaf bud stem cuttings were cultured in the optimum subculture medium (MS+6-BA1.0mg / L+IBA0.2mg / L+2% sucrose). After 30d, the subcultured seedlings were moved into the optimal rooting medium (1 / 2MS+6-BA0.2mg / L+IBA1.0mg / L+2% sucrose) and carried out rooting culture, and transferred to Acclimatized on perlite and sand, and finally transplanted in neutral loam.

Embodiment 2

[0038] Choose the dicotyledonous plant "Red Lantern" sweet cherry with a leaf bud stem section, wash it with running water, peel off the scales outside the leaf bud and the epidermis of the stem section, and put a section of stem with leaf bud into 1:1 (W / V) The garlic aqueous solution was sterilized for 30 minutes, and then immediately inoculated in the optimum primary culture medium of "Red Lantern" sweet cherry (MS+6-BA1.0mg / L+IBA0.1mg / L+3% sucrose). After 30 days, the germinated leaf bud stem cuttings were cultured in the most suitable subculture medium (2 / 3MS+6-BA0.5mg / L+IBA0.3mg / L+GA1.5+2% sucrose). After 40 days, the subcultured seedlings were moved into the optimal rooting medium (1 / 2MS+IBA0.5mg / L+2% sucrose) for rooting culture, and then transferred to perlite and sandy soil for domestication after growing good roots. Finally transplanted in neutral loam. Example 3:

Embodiment 3

[0039] Select the seeds of the dicotyledon licorice, and treat them with 98% concentrated sulfuric acid for 1 hour to soften the hard seed coat, and then fully rinse them with tap water. Sterilize with 0.1% mercuric chloride for 10 minutes under a sterile operating table, rinse with sterile water for 3 to 4 times, then disinfect with 75% ethanol for 40 seconds, and rinse with sterile water for 3 to 4 times. The best medium for primary culture (or inoculation) is MS medium, cultured in the dark for 1 to 2 weeks, and then cultured in the light for one month. The best medium for cultivating stems with leaves to directly take root and become seedlings is: MS+13.5mg / LKH 2 PO 4 +3% sucrose. The rooting and seedling selection is directly grown on the basis of subculture, and after a good root system grows, it is transferred to perlite and sandy soil for domestication, and finally transplanted in neutral loam.

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PUM

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a plant tissue culture support. The support comprises dry sphagnum and de-ionized water, wherein the volume ratio of the dry sphagnum to the de-ionized water is (0.5-1):1. Compared with the prior art, the plant tissue culture support has the beneficial effects that the plant tissue culture support is easy to operate and low in tissue culture cost; energy resources are saved; the working time is shortened, the labor intensity is reduced, the inoculation efficiency is improved, and the pollution probability is reduced; an explant grows robustly, and is high in transplantation survival rate; good fixing effects are achieved; the air permeability is high; a culture medium is long in service cycle; a tissue culture environment can more meet physiological requirements of a plant.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a plant tissue culture support. Background technique [0002] There are two types of physical state of medium for plant tissue culture, liquid and solid. Liquid media are suitable for cell culture, promoting the increase of cell bioproduction or metabolites. Solid medium is mostly used for the cultivation of callus and test-tube plantlets. Support refers to some substances that are artificially added to the medium to support the growth of plant explants. At present, the support of the medium is mostly agar. Agar is a polysaccharide and does not provide any nutrition itself. It is a high molecular compound extracted from seaweed. Agar is only soluble in hot water above 90°C and becomes a sol, and begins to solidify below 40°C to become a solid gel. The coagulation degree of agar is also related to factors such as the time, temperature, and pH value of the high-tempera...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 侯义龙
Owner DALIAN UNIV
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