Plant tissue culture support
A plant tissue culture and support technology, applied in plant regeneration, botany equipment and methods, horticulture, etc., can solve the problems of poor growth, poor fixation of explants, etc., to reduce pollution and reduce tissue culture costs , time-saving effect
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Embodiment 1
[0036]Select the leaf buds of the dicotyledonous vine rose, wash them with running water, peel off the scales outside the buds, sterilize the buds in 1:1 (W / V) garlic aqueous solution for 30 minutes, and inoculate them immediately on the last leaves of the vine rose. Suitable primary culture medium (MS+6-BA0.5mg / L+NAA0.05mg / L+3% sucrose). After 30 days, the germinated leaf bud stem cuttings were cultured in the optimum subculture medium (MS+6-BA1.0mg / L+IBA0.2mg / L+2% sucrose). After 30d, the subcultured seedlings were moved into the optimal rooting medium (1 / 2MS+6-BA0.2mg / L+IBA1.0mg / L+2% sucrose) and carried out rooting culture, and transferred to Acclimatized on perlite and sand, and finally transplanted in neutral loam.
Embodiment 2
[0038] Choose the dicotyledonous plant "Red Lantern" sweet cherry with a leaf bud stem section, wash it with running water, peel off the scales outside the leaf bud and the epidermis of the stem section, and put a section of stem with leaf bud into 1:1 (W / V) The garlic aqueous solution was sterilized for 30 minutes, and then immediately inoculated in the optimum primary culture medium of "Red Lantern" sweet cherry (MS+6-BA1.0mg / L+IBA0.1mg / L+3% sucrose). After 30 days, the germinated leaf bud stem cuttings were cultured in the most suitable subculture medium (2 / 3MS+6-BA0.5mg / L+IBA0.3mg / L+GA1.5+2% sucrose). After 40 days, the subcultured seedlings were moved into the optimal rooting medium (1 / 2MS+IBA0.5mg / L+2% sucrose) for rooting culture, and then transferred to perlite and sandy soil for domestication after growing good roots. Finally transplanted in neutral loam. Example 3:
Embodiment 3
[0039] Select the seeds of the dicotyledon licorice, and treat them with 98% concentrated sulfuric acid for 1 hour to soften the hard seed coat, and then fully rinse them with tap water. Sterilize with 0.1% mercuric chloride for 10 minutes under a sterile operating table, rinse with sterile water for 3 to 4 times, then disinfect with 75% ethanol for 40 seconds, and rinse with sterile water for 3 to 4 times. The best medium for primary culture (or inoculation) is MS medium, cultured in the dark for 1 to 2 weeks, and then cultured in the light for one month. The best medium for cultivating stems with leaves to directly take root and become seedlings is: MS+13.5mg / LKH 2 PO 4 +3% sucrose. The rooting and seedling selection is directly grown on the basis of subculture, and after a good root system grows, it is transferred to perlite and sandy soil for domestication, and finally transplanted in neutral loam.
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