Method for extracting general flavones from chequer-shaped indocalamus leaves

A technology of total flavonoids and rutabaga, which is applied in medical formulas, medical preparations containing active ingredients, food science, etc., can solve the problems of long extraction time, environmental pollution, and low efficiency of total plant flavonoids, and achieve short extraction time, Improve the extraction rate and promote the effect of swelling and cracking

Active Publication Date: 2016-10-12
JIAXING VOCATIONAL TECHN COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creation of highly effective solutions that are able to efficiently remove volatile compounds such as alcoholic substances (ethanol) or alkaloids from plants without causing damage during their drying process. These methods also allow for controlled temperature conditions while maintaining good quality extracted materials.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the efficient extractions of plants containing various compounds from rutweed berbons called “RuO” or "RooYe". Current techniques involve traditional liquid acid hydrothermal extraction processes requiring longer times at different temperatures due to slow reaction rates caused by thermal expansion during cool down period. There also exist issues related to safety concerns associated with these procedures.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment 1

[0018] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to pulverize, and then pass 160 mesh to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, and add PH=8.2 NaCl- at a ratio of 1:20g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 0.2%, is then sealed and put into a microwave extractor for extraction. The power is 500W, the extraction temperature is 100°C, and the extraction time is 4min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.54%.

specific Embodiment 2

[0019] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to pulverize, and then pass 160 mesh to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, and add pH=8.2 NaCl- at a ratio of 1:30g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 0.4%, is then sealed and put into a microwave extractor for extraction. The power is 400W, the extraction temperature is 100°C, and the extraction time is 4min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.62%.

specific Embodiment 3

[0020] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to grind, and then pass 160 meshes to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, add PH=8.2 NaCl- at a ratio of 1:40g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 1.0%, is then sealed and placed in a microwave extractor for extraction. The power is 500W, the extraction temperature is 100°C, and the extraction time is 6min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.88%.

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Abstract

The invention discloses a method for extracting general flavones from chequer-shaped indocalamus leaves. The method includes the steps that fresh chequer-shaped indocalamus leaves are washed to be clean, dried, crushed and sieved with a 160-mesh sieve, and chequer-shaped indocalamus leaf powder is obtained; then, the chequer-shaped indocalamus leaf powder and a NaCl-NaOH mixed solution are mixed to be uniform in certain proportion, the mixture is placed into microwave extraction equipment to be extracted twice in a closed high-pressure environment, after extraction is completed, an extracting solution is filtered, constant volume is conducted after the extracting solution is combined, and the content of the general flavones in the chequer-shaped indocalamus leaves is measured. According to the method, the NaCl-NaOH mixed solution serves as extraction solvent for the first time, and the general flavones in the chequer-shaped indocalamus leaves are extracted in the closed high-pressure environment in combination with a microwave-assisted method. The method has the advantages of being rapid, efficient, high in extraction rate, environmentally friendly and beneficial for subsequent separation and purification work of the general flavones in the chequer-shaped indocalamus leaves.

Description

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Claims

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Application Information

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Owner JIAXING VOCATIONAL TECHN COLLEGE
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