Method for extracting general flavones from chequer-shaped indocalamus leaves
A technology of total flavonoids and rutabaga, which is applied in medical formulas, medical preparations containing active ingredients, food science, etc., can solve the problems of long extraction time, environmental pollution, and low efficiency of total plant flavonoids, and achieve short extraction time, Improve the extraction rate and promote the effect of swelling and cracking
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specific Embodiment 1
[0018] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to pulverize, and then pass 160 mesh to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, and add PH=8.2 NaCl- at a ratio of 1:20g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 0.2%, is then sealed and put into a microwave extractor for extraction. The power is 500W, the extraction temperature is 100°C, and the extraction time is 4min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.54%.
specific Embodiment 2
[0019] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to pulverize, and then pass 160 mesh to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, and add pH=8.2 NaCl- at a ratio of 1:30g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 0.4%, is then sealed and put into a microwave extractor for extraction. The power is 400W, the extraction temperature is 100°C, and the extraction time is 4min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.62%.
specific Embodiment 3
[0020] Rinse the picked fresh ruo leaves, dry them naturally, and put them in an oven at 50°C to dry them to reduce the moisture content of ruo leaves to less than 5%. Put the dried ruo leaves into a high-speed pulverizer to grind, and then pass 160 meshes to obtain ruo leaf powder; take 0.5 g ruo leaf powder into the microwave extraction tank, add PH=8.2 NaCl- at a ratio of 1:40g / mL The NaOH mixed solution, in which the mass concentration of NaCl is 1.0%, is then sealed and placed in a microwave extractor for extraction. The power is 500W, the extraction temperature is 100°C, and the extraction time is 6min. Extract twice, filter the extract obtained twice into a 50mL volumetric flask, make a constant volume, and use the "sodium nitrite-aluminum nitrate-sodium hydroxide colorimetric method" to determine the absorbance of the extract at 510nm to calculate the total flavonoids The extraction rate reached 2.88%.
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