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Indole-degrading compound bacterial agent and reaction device thereof

A technology of compound bacterial agent and reaction device, which is applied in bacteria, microorganism-based methods, water/sludge/sewage treatment, etc. The effect of addressing limitations

Active Publication Date: 2016-10-12
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the limitations of existing methods for treating indole and the problem of low treatment efficiency of existing bacterial agents, the present invention provides a composite bacterial agent for degrading indole and a reaction device thereof

Method used

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  • Indole-degrading compound bacterial agent and reaction device thereof
  • Indole-degrading compound bacterial agent and reaction device thereof
  • Indole-degrading compound bacterial agent and reaction device thereof

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specific Embodiment approach 1

[0033] Specific embodiment 1: In this embodiment, the compound microbial agent for degrading indole is prepared from three strains of indole-degrading microorganisms: Pseudomonas monteilii QM (pseudomonas monteilii QM), Bacillus L1 and Tolumonas G1 , the specific preparation method is as follows:

[0034] 1. Inoculate Pseudomonas monteilii QM (pseudomonas monteilii QM), Bacillus L1 and Tolumonas G1 on liquid medium for activation respectively;

[0035] 2. Pick three activated single colonies and inoculate them on the liquid medium respectively, and enrich them for 1-3 days under aerobic conditions with a temperature of 20°C and a shaking speed of 150r / min;

[0036] 3. Inoculate the three kinds of single bacteria after the enrichment culture into the liquid medium respectively, and cultivate until the number of bacteria is 10 9 ~10 10 Each / mL, and then Pseudomonas monteilii QM (pseudomonas monteilii QM), Bacillus L1 and Tolumonas G1 were mixed at a volume ratio of 1:2:1 to ob...

specific Embodiment approach 2

[0042] Specific implementation mode two: as figure 1 As shown, the reaction device for degrading indole provided by the present embodiment is composed of an aeration mixing reaction zone, an aeration device 5, a bacterial agent feeder 1 equipped with a composite bacterial agent for degrading indole, an overflow An integrated device consisting of the flow area 3, the inclined plate sedimentation area, the excess sludge discharge pipe 14 and the sludge return device, wherein:

[0043] The bacteria material feeder 1 is arranged above the aeration mixing reaction zone;

[0044] The aeration mixed reaction zone is provided with a water inlet pipe 2 on the side close to the bacterial material feeder 1, and an overflow zone 3 is arranged on the side away from the water inlet pipe, and the lower part of the aerated mixed reaction zone is a slanted plate sedimentation zone;

[0045]The aeration device 5 is located in the aeration mixed reaction zone, and the air is fed through the aer...

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Abstract

The invention discloses an indole-degrading compound bacterial agent and a reaction device thereof. The compound bacterial agent is prepared from three indole-degrading microbial strains including pseudomonas monteilii QM, bacillus L1 and Tolumonas G1. The reaction device comprises an aeration mixing reaction area, an aeration device, a bacterial agent feeder with the indole-degrading compound bacterial agent, an overflow area, an inclined-plate precipitation area, a residual sludge discharge pipe and a sludge backflow device. The indole-degrading compound bacterial agent disclosed by the invention has strong practicability; under the joint effect with the compound bacterial agent, the pollutant degrading efficiency of aerobic active sludge is high; the compound bacterial agent can adapt to the environment within short time so as to culture and acclimatize an efficient flora suitable for indole wastewater; the indole and COD detected in the water both can reach the standard; and the problems of limitation of existing methods in treating nitrogen heterocyclic ring organic matter indole and low treatment efficiency of existing bacterial agents are solved.

Description

technical field [0001] The invention belongs to the field of water treatment and relates to a composite microbial agent for degrading indole and a reaction device thereof. Background technique [0002] Nitrogen Heterocyclic Compounds (NHCs) widely exist in industrial pollutants such as coking, dyes, medicines, cosmetics, pesticides and petroleum smelting. It has a pungent smell, is not easy to be biodegraded and metabolized in the natural environment, has an inhibitory effect on microorganisms, and some have "three causes" effects such as teratogenic, carcinogenic, and mutagenic. It can exist in soil and water stably for a long time, and has potential harm to the environment and human health.  [0003] As a nitrogen-containing heterocyclic organic compound, indole has a strong fecal odor when the concentration is high, strong and long-lasting diffusion, and strong biological toxicity. Due to its own characteristics, the presence of indole in water will greatly increase t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C12R1/07C12R1/01C02F101/38
CPCC02F3/341C02F2101/38C12N1/20
Inventor 王广智于洋洋王卓然丁杰
Owner HARBIN INST OF TECH
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