The invention provides a microbe water purifying additive and a preparation method thereof and an application thereof. The preparation of the microbe water purifying additive is as follows: aspergillus niger CCAM080003 is subject to activation, expansion and fermentation culture, and the culture materials collected are dried and crushed, and the additive is prepared. The microbe water purifying additive of the invention contains hydrolase such as hot activity cellulase, xylanase, dextranase, prolease and lipase, etc., and can accelerate the decomposition of organic matters in the sewage and improve the water treatment efficiency, so the microbe water purifying additive is applied to the water purification treatment in ponds, sewage treatment plants and scenic water, etc. The microbe water purifying additive of the invention can be joined with the microbe purifying agent to purify water; compared with the independent use of the microbe purifying agent, the addition of the water purifying additive can greatly improve the speed of purifying the water and obviously improving the purifying effect.