Microorganism water purifying assistant, and preparation and use thereof
A technology for microorganisms and water purification, applied in the field of microbial water purification aids and their preparation, can solve problems such as low water purification efficiency and achieve the effects of good effect, accelerated decomposition and increased speed
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Embodiment 1
[0041] Embodiment 1 microbial water purification aid
[0042] A kind of microbial water purification aid, its preparation method is as follows:
[0043] 1. Experimental materials
[0044] 1.1 Preparation of potato medium
[0045] Boil 200g of peeled potatoes with 1000ml of water for half an hour, filter with double gauze, take the filtrate, add 20g of sucrose, add water to make up 1000ml, and add 20g of agar strips, the pH value is natural. The potato culture medium was made into a test tube slant according to the general method, sterilized by high pressure steam at 121°C for 30 minutes, and then set aside.
[0046] 1.2 Preparation of seed expansion medium
[0047] Take 98g bran, 2g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix with 101mL of water, the pH value is natural, 121 ℃, after 30min high-pressure steam sterilization, set aside.
[0048] 1.3 Preparation of Enzyme Production Medium
[0049] Take 98g bran, 2g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix...
Embodiment 2
[0056] Embodiment 2 microbial water purification aid
[0057] A kind of microbial water purification aid, its preparation method is as follows:
[0058] 1. Experimental materials
[0059] 1.1 Preparation of potato medium
[0060] Boil 200g of peeled potatoes with 1000ml of water for half an hour, filter with double gauze, take the filtrate, add 20g of sucrose, add water to make up 1000ml, and add 15g of agar strips, the pH value is natural. The potato culture medium was made into a test tube slant according to the general method, sterilized by high pressure steam at 121°C for 30 minutes, and then set aside.
[0061] 1.2 Preparation of seed expansion medium
[0062] Take 55g bran, 45g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix with 101mL of water, the pH value is natural, 121 ℃, after 30min high-pressure steam sterilization, set aside.
[0063] 1.3 Preparation of Enzyme Production Medium
[0064] Take 55g bran, 45g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4...
Embodiment 3
[0071] The enzyme production effect of the enzyme production medium of embodiment 3 different proportions of wheat bran and corncob powder
[0072] 1. Definition of enzyme activity
[0073] 1.1 Determination of xylanase activity
[0074] Enzyme activity definition: using xylan as a substrate, under the conditions of 40°C and pH 4.6, the enzymatic hydrolysis of 1 mg of xylose per hour is defined as an enzyme activity unit, and the unit is U / g.
[0075] 1.2 Cellulase activity assay method
[0076] Definition of enzyme activity: using sodium carboxymethylcellulose as a substrate, under the conditions of 40°C and pH 4.6, 1 mg of glucose produced by enzymatic hydrolysis per hour is defined as an enzyme activity unit (U), and the enzyme activity unit is expressed in U / g express.
[0077] 1.3 Determination of amylase activity
[0078] Definition of enzyme activity: using starch as a substrate, under the conditions of 40°C and pH 6.8, 1 mg of glucose produced by enzymatic hydrolys...
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