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Microorganism water purifying assistant, and preparation and use thereof

A technology for microorganisms and water purification, applied in the field of microbial water purification aids and their preparation, can solve problems such as low water purification efficiency and achieve the effects of good effect, accelerated decomposition and increased speed

Inactive Publication Date: 2008-12-24
东莞市华中生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when microbial water purifiers are added to polluted water bodies, the extracellular enzymes secreted by the microbial flora into the environment are often affected by many environmental factors, such as enzyme adsorption, enzyme denaturation, and enzyme biodegradation, etc. However, it is limited, which leads to low water purification efficiency after microbial water purifiers are put into polluted water bodies, especially in the early stage of putting in, when the growth of microbial flora is not high, this environment affects the action of extracellular enzymes, which in turn leads to water purification Especially low efficiency
[0005] Therefore, adding corresponding enzymes to the water treatment project of microbial water purifiers can theoretically promote and improve the water purification efficiency of microbial water purifiers. At present, there is no report on the application of enzymes in sewage treatment in China.

Method used

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  • Microorganism water purifying assistant, and preparation and use thereof
  • Microorganism water purifying assistant, and preparation and use thereof
  • Microorganism water purifying assistant, and preparation and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 microbial water purification aid

[0042] A kind of microbial water purification aid, its preparation method is as follows:

[0043] 1. Experimental materials

[0044] 1.1 Preparation of potato medium

[0045] Boil 200g of peeled potatoes with 1000ml of water for half an hour, filter with double gauze, take the filtrate, add 20g of sucrose, add water to make up 1000ml, and add 20g of agar strips, the pH value is natural. The potato culture medium was made into a test tube slant according to the general method, sterilized by high pressure steam at 121°C for 30 minutes, and then set aside.

[0046] 1.2 Preparation of seed expansion medium

[0047] Take 98g bran, 2g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix with 101mL of water, the pH value is natural, 121 ℃, after 30min high-pressure steam sterilization, set aside.

[0048] 1.3 Preparation of Enzyme Production Medium

[0049] Take 98g bran, 2g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix...

Embodiment 2

[0056] Embodiment 2 microbial water purification aid

[0057] A kind of microbial water purification aid, its preparation method is as follows:

[0058] 1. Experimental materials

[0059] 1.1 Preparation of potato medium

[0060] Boil 200g of peeled potatoes with 1000ml of water for half an hour, filter with double gauze, take the filtrate, add 20g of sucrose, add water to make up 1000ml, and add 15g of agar strips, the pH value is natural. The potato culture medium was made into a test tube slant according to the general method, sterilized by high pressure steam at 121°C for 30 minutes, and then set aside.

[0061] 1.2 Preparation of seed expansion medium

[0062] Take 55g bran, 45g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4 Mix with 101mL of water, the pH value is natural, 121 ℃, after 30min high-pressure steam sterilization, set aside.

[0063] 1.3 Preparation of Enzyme Production Medium

[0064] Take 55g bran, 45g corn cob, 0.5g (NH4) 2 SO 4 , 0.5g KH 2 PO 4...

Embodiment 3

[0071] The enzyme production effect of the enzyme production medium of embodiment 3 different proportions of wheat bran and corncob powder

[0072] 1. Definition of enzyme activity

[0073] 1.1 Determination of xylanase activity

[0074] Enzyme activity definition: using xylan as a substrate, under the conditions of 40°C and pH 4.6, the enzymatic hydrolysis of 1 mg of xylose per hour is defined as an enzyme activity unit, and the unit is U / g.

[0075] 1.2 Cellulase activity assay method

[0076] Definition of enzyme activity: using sodium carboxymethylcellulose as a substrate, under the conditions of 40°C and pH 4.6, 1 mg of glucose produced by enzymatic hydrolysis per hour is defined as an enzyme activity unit (U), and the enzyme activity unit is expressed in U / g express.

[0077] 1.3 Determination of amylase activity

[0078] Definition of enzyme activity: using starch as a substrate, under the conditions of 40°C and pH 6.8, 1 mg of glucose produced by enzymatic hydrolys...

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PUM

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Abstract

The invention provides a microbe water purifying additive and a preparation method thereof and an application thereof. The preparation of the microbe water purifying additive is as follows: aspergillus niger CCAM080003 is subject to activation, expansion and fermentation culture, and the culture materials collected are dried and crushed, and the additive is prepared. The microbe water purifying additive of the invention contains hydrolase such as hot activity cellulase, xylanase, dextranase, prolease and lipase, etc., and can accelerate the decomposition of organic matters in the sewage and improve the water treatment efficiency, so the microbe water purifying additive is applied to the water purification treatment in ponds, sewage treatment plants and scenic water, etc. The microbe water purifying additive of the invention can be joined with the microbe purifying agent to purify water; compared with the independent use of the microbe purifying agent, the addition of the water purifying additive can greatly improve the speed of purifying the water and obviously improving the purifying effect.

Description

technical field [0001] The invention belongs to the technical field of microbial environmental protection, and in particular relates to a microbial water purification aid and its preparation method and application. Background technique [0002] The purification of sewage (mainly referring to organic wastewater) depends on the action of microorganisms whether it is a natural process or industrial treatment. Microbial water purifier belongs to a type of EM. EM is effective microbial flora, which is a complex of beneficial microorganisms, which can purify water quality, recycle water, reduce pathogenic derivatives and bad algae, decompose residual pesticides, and purify wells. Water, pond water pollution, etc., while the microbial water purifier itself does not contain any chemical harmful substances, has no toxic side effects, and does not pollute the environment. Therefore, live microbial water purifiers are more and more widely used. [0003] The process of degrading (organ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C02F3/34C12R1/685
Inventor 梁运祥王绩葛向阳钟旭胜李卓杰
Owner 东莞市华中生物科技有限公司
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