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A serum or plasma miRNA marker associated with childhood obesity and its application

A marker and serum technology, applied in the field of serum or plasma miRNA markers, can solve the problems that tissue miRNA cannot meet the needs of early diagnosis, cannot be used for screening and prevention, etc., achieve accurate, objective and reliable results, and improve the possibility and feasibility. , pain relief effect

Active Publication Date: 2019-05-24
NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although miRNA in tumor tissue can be used as a new tumor marker, miRNA in tumor tissue must be obtained through surgery or puncture, so tissue miRNA cannot meet the needs of early diagnosis and cannot be used for extensive screening and prevention.

Method used

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  • A serum or plasma miRNA marker associated with childhood obesity and its application
  • A serum or plasma miRNA marker associated with childhood obesity and its application
  • A serum or plasma miRNA marker associated with childhood obesity and its application

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Experimental program
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Effect test

Embodiment 1

[0044] 1. Test materials: Trizol reagent, TaqMan reversal rate kit, UniversalMaster Mix II was purchased from Life technologies; chloroform and absolute ethanol were purchased from Sinopharm Pharmaceutical Co., Ltd.; DEPC water was purchased from Shanghai Biyuntian Biotechnology Co., Ltd.

[0045] 2. Sample collection and sorting of sample data:

[0046] The present invention uses a total of 352 children’s blood samples, including 136 samples from the obese group and 216 samples from the normal group; there is no significant difference in age between the two groups of samples (P> 0.05), BMI is significantly different (P <0.001), in addition, blood lipids, total cholesterol, low-density lipoprotein and high-density lipoprotein were significantly different between the two groups (P <0.05). 15 serum samples of obese children and normal children were selected. After total RNA extraction, every 5 samples were mixed into one sample for deep sequencing analysis (Deepsequencing).

[0047] 3...

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PUM

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Abstract

The invention discloses childhood obesity-related serum or plasma miRNA markers and a use thereof. The markers comprise one or more of miR-26b, miR-222, miR-146a and miR-146b. The invention also provides a childhood obesity early diagnosis kit. The markers can diagnose childhood obesity on the micro-level and provide early warning of a childhood obesity risk. The markers can be operated simply, produce accurate, objective and reliable results, can distinguish an obese group and a normal group, can accurately and sensitively evaluate childhood obesity and provide early warning of a childhood obesity risk, prevent use of a blood sample and other tissue samples, greatly improve the possibility and feasibility of clinical application and provide guidance and basis for clinicians to make early prevention and intervention schemes.

Description

Technical field [0001] The invention relates to the fields of genetic engineering and medicine, in particular to a serum or plasma miRNA marker related to childhood obesity and its application. Background technique [0002] As the country becomes prosperous and powerful, the living standards of our residents are improving day by day. Especially since the reform and opening up, people's lifestyles have also undergone tremendous changes. The improvement of the economic level and the acceleration of the mechanization process have led to a decline in the labor intensity and physical activity of the residents, and the improvement of living standards has led to the mainstream diet of high fat and low carbohydrates; the incidence of obesity has rapidly increased among all types of urban and rural people, becoming a serious problem Epidemics that endanger the health of residents. The harm of the obesity epidemic has also been increasingly recognized by the general public. Obesity is not...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 郭锡熔尤梁惠季晨博崔县伟祝丽君李沄
Owner NANJING MATERNITY & CHILD HEALTH CARE HOSPITAL
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