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A biological method for removing urushiol hypersensitivity

An allergic and biological method technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the development disadvantages of raw lacquer industry, allergic reactions and other problems, and achieve the solution of allergenicity problems, simple process operation, easy-to-implement effects

Active Publication Date: 2019-04-19
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the allergenic substances in raw lacquer, during the construction process, a large number of personnel directly contact or smell the smell of raw lacquer, and all will have different degrees of allergic reactions, resulting in fewer and fewer technicians who can use raw lacquer. With today's rapid development, under the condition of alternatives and fierce competition, the development of raw lacquer industry is bound to be at a disadvantage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The first step, slant cultivation

[0029] Rapeseed yellow single cell Xanthomonas campestris Inoculate on a solid slant medium, the solid medium is: 5.0g peptone, 3.0g beef extract, 5.0g NaCl, 15.0g agar, 1.0L distilled water, pH7.0; Cultivate for 5 days to obtain slant strains;

[0030] The second step is to prepare the seed culture medium

[0031] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is: 5.0g of peptone, 3.0g of beef extract, 5.0g of NaCl, Distilled water 1.0L, pH7.0; at 30-35°C, 180rpm shaker culture for 5-7 days to obtain yellow single cells of wild rape Xanthomonas campestris seed culture fluid;

[0032] The third step, biotransformation

[0033] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the substrate medium, and culture on a shaker at 180rpm at 30-35°C for 1-2 days, ferment After...

Embodiment 2

[0038] The first step, slant cultivation

[0039] Streptomyces griseus var. rust Streptomyces griseus var. ferrugineus Inoculate on a solid slant medium, the slant medium is: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH; under the condition of 28-30°C, static culture for 5 days to obtain slant strains;

[0040] The second step is to prepare the seed culture medium

[0041] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is 1.0L of potato extract, 20.0g of glucose, and the pH is natural; at 30~ Under the condition of 35°C, cultivate it on a shaker at 180rpm for 5-7 days to obtain the rust-colored variant of Streptomyces griseus Streptomyces griseus var. ferrugineus seed culture fluid;

[0042] The third step, biotransformation

[0043] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the s...

Embodiment 3

[0045] The first step, slant cultivation

[0046] silvery mildew Cunninghamella elegans The strains were inoculated on a solid slant medium, the slant medium was: 1.0L of potato extract, 20.0g of glucose, 15.0g of agar, natural pH; at 28-30°C, static culture for 5 days to obtain slant strains;

[0047] The second step is to prepare the seed culture medium

[0048] Take the slant bacteria in the first step, under aseptic conditions, take 5 rings of bacteria, and transfer them to the liquid medium, the liquid medium is: 1.0L of potato extract, 20.0g of glucose, natural pH; at 30 Cultivate in a shaker at 180rpm at ~35°C for 5-7 days to produce Yazhi Xiaogram silvery mildew Cunninghamella elegans seed culture fluid;

[0049] The third step, biotransformation

[0050] Use the seed culture solution in the second step, add 0.4-1% urushiol at a volume ratio of 4-6%, inoculate the substrate medium, and culture on a shaker at 180rpm at 30-35°C for 1-2 days, ferment After the orig...

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PUM

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Abstract

The invention relates to a biological method for removing urushiol sensitization of Rhus verniciflua Stokes and belongs to the field of natural product and biotransformation. The biological method is about screening of biotransformation strains taking the allergy source (urushiol) of raw lacquer as the substrate, the Streptomyces griseus var. ferrugineus strain capable of efficiently degrading urushiol is induced and screened out by the substrate, and the urushiol conversion rate can reach 93.38%. The biological method is simple and convenient to operate and high in urushiol conversion rate and can be widely applied to urushiol sensitization removal and development of no-sensitization Rhus verniciflua Stokes active extracts.

Description

technical field [0001] The invention relates to a biological method for removing urushiol allergy, belonging to the field of natural products and biotechnology. Background technique [0002] The secretion of the phloem of Anacadiaceae (Rhus verniciflua Stokes), raw lacquer, also known as "earth lacquer", "national lacquer" or "big lacquer", is the product with the most economic value in lacquer trees. It has a long history of more than seven thousand years [1]. Raw lacquer is a milky white gelatinous liquid, which turns brown once exposed to air, and the surface dries up and hardens after a few hours to form a lacquer. Raw lacquer is mainly used in the coating industry and has extremely high applicable economic value, because of its high gloss, high hardness, corrosion resistance, wear resistance, acid resistance, solvent resistance, heat resistance, good water insulation and insulation, high gloss waitability, anti-corrosion Chemical corrosion and other characteristics, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/64C12R1/545C12R1/845C12R1/72C12R1/645
CPCA62D3/02C12N1/14C12N1/20
Inventor 王成章陈虹霞
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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