Complement fixation anti-sperm antibody enzyme-linked immunosorbent assay method

An enzyme-linked immunosorbent assay and anti-sperm antibody technology, applied in the field of enzyme immunoassay
CN106093376AInactive Publication Date: 2016-11-09TIANJIN BAODI HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN BAODI HOSPITAL
Publication Date
2016-11-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a complement fixation anti-sperm antibody enzyme-linked immunosorbent assay method and belongs to the technical field of enzyme immunoassay. The method comprises the following steps: combining IgG, IgA, IgM, IgD and IgE type anti-sperm antibodies in serum with a reaction hole coated sperm antigen, then combining with a complement C3C to form antigen-antibody-complement C3C immune complex, adding anticomplement C3C labelling enzyme, reacting, then adding an enzyme substrate solution, and developing. The method disclosed by the invention can detect the IgG, IgA, IgM, IgD and IgE type anti-sperm antibodies in the serum at the same time, is easy to operate, rapid, sensitive, accurate and cheap, is applicable to total anti-sperm antibody detection and can be taken as an important examination index of infertility.
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Description

technical field

[0001] The invention relates to an enzyme-linked immunosorbent detection method for complement fixation in serum, which belongs to the technical field of enzyme immunoassay. Background technique

[0002] Anti-sperm antibody (AsAb) is a complex pathological product that can affect both men and women. The exact cause is not yet fully understood. Men’s sperm and seminal plasma are all specific antigens for women. After contact with blood, both men and women can Cause immune reaction, produce corresponding antibody, prevent sperm from combining with egg, and cause infertility, in the blood and seminal plasma of 5-10% infertile men, sperm agglutinating antibody and sperm braking antibody do exist. When the serum and seminal plasma with anti-sperm antibodies are mixed with normal sperm, the sperm will undergo head-to-head, tail-to-tail or mixed agglutination, or in the presence of complement, this serum and seminal plasma can make swimming Sperm stops moving or sh...

Claims

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