Ventilation net pipe for cells

Abstract

The invention discloses a ventilation net pipe for cells. The ventilation net pipe is arranged in a cell culture tank and comprises a stirring center, wherein the stirring center comprises a vertical stirring shaft and a stirring drum coaxially connected at the bottom end of the stirring shaft; a first filter is connected at the bottom end of the stirring drum in a sleeving manner and comprises a first lower end plate, a first upper end plate and a first filter net pipe; the first lower end plate is fixed and connected at the bottom end of the stirring drum in a sleeving manner; the first upper end plate is arranged above the first lower end plate and is fixed and connected with the stirring drum in a sleeving manner; the first filter net pipe connects the external circumference of the first upper end plate with that of the first lower end plate; stirring paddles fixed together with the external circumference of the stirring drum are arranged above the first filter; a ventilation pipe is arranged in the radial center of the stirring center and is divided into a ventilation pipe body and a hook part; the ventilation pipe body vertically penetrates through the stirring center; one end of the hook part is connected with the bottom end of the ventilation pipe body and the other end stretches upward into the bottom of the first filter from the bottom surface of the first lower end plate.

Description

technical field [0001] The invention relates to a cell ventilation network pipe in the field of medical equipment. Background technique [0002] Mammalian cell culture refers to the technique of maintaining cell growth and proliferation with culture medium under in vitro conditions of cells. A microcarrier cell reactor usually consists of a culture tank and a vent tube. The culture tank is filled with culture fluid and cells to provide a growth environment for the cells. The vent tube is used to deliver gas to the culture tank. During the culture process, the cells absorb the nutrients in the culture fluid and the dissolved oxygen in the gas for their own growth. need. [0003] There are mainly two methods for culturing and aeration of animal cell microcarriers at present. One is to use the method of surface oxygen supply and stirring. The disadvantage of this method is that the gas-liquid ratio surface is too small, and the oxygen supply of surface ventilation is often i...

AI Technical Summary

Problems solved by technology

One is to use the method of surface oxygen supply and stirring. The disadvantage of this method is that the gas-liquid ratio surface is too small, and the oxygen supply of surface ventilation is often insufficient.

Another method is to use deep aeration, that is, a ventilation tube is inserted into the bottom of the culture tank to supply air. The oxygen supply of this method can meet the needs of cell growth, but because the bubbles will trap cells during the rising process, so When the bubbles burst during ascent, the shear force generated by the rupture will tear the cells apart and cause cell death, which will cause a large number of cells to be damaged throughout the culture cycle

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