A strain of Serratia with rich phosphorus, organic phosphorus degradation and inhibition of plant pathogenic fungi

A plant pathogenic fungus, Serratia technology, applied in the field of microbiology, can solve the problems that Serratia has not been reported, and achieve the effect of high stress resistance, simple cultivation method and broad market prospect

Active Publication Date: 2019-06-18
沃达农业科技股份有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many reports at home and abroad about the high-yielding chitinase of Serratia marcescens and the red non-diffusing pigment-protozoin, which has the functions of antibacterial, immune regulation, and tumor suppression. Glyphosate degradation effect of Serratia has not been reported

Method used

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  • A strain of Serratia with rich phosphorus, organic phosphorus degradation and inhibition of plant pathogenic fungi
  • A strain of Serratia with rich phosphorus, organic phosphorus degradation and inhibition of plant pathogenic fungi
  • A strain of Serratia with rich phosphorus, organic phosphorus degradation and inhibition of plant pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026]Water samples from Wuhan Shahu Lake and its surrounding areas were collected and added to LB medium, and enriched at 28°C and 150r / min for 24h. The above bacterial suspension was inoculated into the fermentation medium containing glyphosate 200mg / L according to the inoculum amount of 5%-10%, and after 28°C 150r / min enrichment culture for 3 days, it was transferred to the same medium, under the same conditions Continue to cultivate, and gradually increase the glyphosate content to 1000mg / L during the passage and acclimation process; streak the 5-passage bacterial solution on LB solid medium, culture at 28°C for 24-48h, according to the formation of colonies on the medium , pick well-growing colonies and inoculate them in LB culture solution containing 50mg / L glyphosate (diluted 5 times), culture at 150r / min at 28°C for 72h, and measure by the acetylcholinease inhibition rate method (GB / T5009.199-2003) The content of organophosphorus pesticides was calculated, and the degr...

Embodiment example 2

[0029] After the purified Serratia sp.MEW06 strain was activated overnight, adjust the OD of the bacterial solution 600 =1.0, according to the inoculum size of 4%, it is transferred to culture in the fermentation broth containing a certain concentration of glyphosate or dimethoate, and the degradation rate is measured by sampling at 1d, 3d, and 5d. The formulation of the fermentation medium is as follows: peptone 2g / L, yeast extract 1g / L, NaCl 2g / L, pH7.0-7.2. The results showed that Serratia sp.MEW06 had an excellent effect on the degradation of dimethoate. When treated with low concentration of dimethoate (50mg / L), the concentration of dimethoate was almost undetectable in the supernatant after 5 days of culture, and the degradation rate almost reached 100%. After treating high-concentration dimethoate (200mg / L) and culturing for 7 days, the degradation rate was determined to reach 90.12%. The ability of MEW06 to treat glyphosate was weaker than that of dimethoate, but at t...

Embodiment example 3

[0031] Get the single colony of Serratia in Example 1 and inoculate it into LB medium for overnight activation. The formula of LB medium is as follows: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH7.0-7.2, press 2% of the inoculum was inoculated in PAM medium with a phosphorus content (calculated as P) of 10 mg / L and 20 mg / L respectively, and samples were taken at 0, 12, 24, 48, and 72 hours to determine the phosphorus accumulation rate. The PAM medium formula For: CH 3 COONa 5g / L, NH 4 Cl2g / L, KH 2 PO 4 0.022~0.0878g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCl 2 0.2g / L, 1000mL deionized water, pH7.0~7.2. The results are shown in Table 1. When the phosphorus content in the solution was 10 mg / L, after 24 hours of enrichment, the inorganic phosphorus content was basically not detected in the solution, the phosphorus enrichment rate reached 99.94%, and the phosphorus content in the solution was 20 mg / L. After 24 hours of enrichment, the phosphorus enrichment rate reached 63.79%. T...

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Abstract

The invention discloses a serratia strain which is rich in phosphorus, degrades organic phosphorus and inhibits plant pathogenic fungi, and belongs to the technical field of microorganisms. the strain Serratia sp.MEW06 is preserved in the China Type Culture Collection Center, and the preservation number is CCTCC NO.M 2015779. The Serratia bacterium of the present invention has a short-term and high-efficiency phosphorus-enriching ability, which can be used to prepare biological fertilizers, and also has the effect on pathogenic fungi of crops. Disease suppression, can be applied to microbial control, at the same time, the bacterial strain has a good degradation effect on organophosphorus pesticide glyphosate and dimethoate, can effectively remove organophosphorus pesticide pollution, Serratia of the present invention has a wide range of applications scope.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Serratia MEW06 and the application of the strain in phosphorus enrichment, organic phosphorus degradation and inhibition of plant pathogenic fungi. Background technique [0002] Organophosphorus pesticides are a major category of pesticides today, accounting for about 80% of pesticide products. Because of their economical, efficient, and rapid control of agricultural diseases, insect pests, and weeds, they have been mass-produced and widely used at home and abroad. However, due to the expanded production of organophosphorus pesticide chemical plants, the amount of wastewater discharged during the production process is large, the COD content is extremely high, the pollutant components are complex, the toxicity is high, and it is difficult to biodegrade, causing great harm to the environment. Moreover, after the pesticide is applied, it will spread from the appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/425
CPCC12N1/205C12R2001/425
Inventor 倪红万娟刘诚唐梦君邵闯
Owner 沃达农业科技股份有限公司
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