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Rapid propagation method of kudzu roots

A fast, rhizome technology, applied in the field of plant tissue culture, to achieve the effect of broad application prospects and short culture period

Inactive Publication Date: 2016-12-07
张玉薇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent with the application number CN201110029788.5 discloses a rapid propagation method of Pueraria lobata, which uses young shoots as explants to induce culture, and the applicant has not yet seen a report on the rapid propagation of Pueraria lobata with shoot tips as explants

Method used

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  • Rapid propagation method of kudzu roots
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Embodiment 1

[0018] The rapid propagation method of Pueraria lobata in this embodiment selects 1 centimeter Pueraria lobata stem tip, soaks the stem tip in 1% sodium hypochlorite solution for 10 minutes, then inoculates it in the bud induction medium, controls the light to be 1800LUX~1878LUX, and the temperature The temperature is 23℃~25℃. After new shoots grow, inoculate into the rooting medium, control the light to 1600LUX~1670LUX, and the temperature is 22℃~26℃. After the roots grow 2 cm, inoculate into the strong seedling medium , control the light to 1917LUX ~ 1983LUX, the temperature is 24 ° C ~ 27 ° C, when the seedlings grow 4 leaves, open the cover of the culture bottle, harden the seedlings in the greenhouse for 3 days, finally take out the seedlings, wash off the medium, and transplant into the field.

[0019] In this embodiment, the bud induction medium uses MS medium as the basic medium, and 0.25 mg / liter 2,4-dichlorophenoxyacetic acid is added; the rooting medium uses 1 / 2 MS ...

Embodiment 2

[0021] The rapid propagation method of Pueraria lobata in this embodiment selects 1.3 centimeters of Pueraria lobata stem tips, soaks the stem tips in a 1% sodium hypochlorite solution for 20 minutes, then inoculates them in the bud induction medium, controls the light to be 1990LUX~2000LUX, and the temperature 23°C-25°C, after the new shoots grow, inoculate into the rooting medium, control the light to 1670LUX-1685LUX, and the temperature is 22°C-26°C, after the roots grow 2 cm, then inoculate into the strong seedling medium , control the light to 1978LUX ~ 2000LUX, the temperature is 24 ° C ~ 27 ° C, when the seedlings grow 4 leaves, open the cover of the culture bottle, harden the seedlings in the greenhouse for 2 days, finally take out the seedlings, wash off the medium, and transplant into the field.

[0022] In this embodiment, the bud induction medium uses MS medium as the basic medium, and 0.35 mg / liter 2,4-dichlorophenoxyacetic acid is added; the rooting medium uses 1...

Embodiment 3

[0024] The rapid propagation method of Pueraria lobata in this embodiment selects 2 centimeters of Pueraria lobata stem tips, soaks the stem tips in a 1% sodium hypochlorite solution for 16 minutes, then inoculates them in the bud induction medium, controls the light to be 1880LUX~1913LUX, and the temperature The temperature is 23℃~25℃. After the new shoots grow, inoculate into the rooting medium, control the light to 16740LUX~1700LUX, and the temperature is 22℃~26℃. After the roots grow 2 cm, inoculate into the strong seedling medium , control the light to 1750LUX ~ 17900LUX, the temperature is 24 ° C ~ 27 ° C, when the seedlings grow 5 leaves, open the cover of the culture bottle, harden the seedlings in the greenhouse for 3 days, finally take out the seedlings, wash off the medium, and transplant into the field.

[0025] In the present embodiment, the bud induction medium takes MS medium as the basic medium, and adds 0.3 mg / liter 2,4-dichlorophenoxyacetic acid; the rooting ...

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Abstract

The invention discloses a rapid propagation method of kudzu roots, and relates to the technical field of plant tissue culture. Kudzu root tips are selected as the explant, an MS culture medium serves as the basic culture medium for a shoot induction medium, and 2,4-dichlorophenoxyacetic acid with concentration of 0.25-0.35 mg / L is added; a 1 / 2 MS culture medium serves as the basic culture medium for a rooting culture medium, and naphthylacetic acid with concentration of 0.25-0.3 mg / L and gibberellin with concentration of0.1-0.2 mg / L are added; a 2 / 3 MS culture medium serves as a basic culture medium for a seedling strengthening culture medium, and naphthylacetic acid with concentration of 0.3-0.5 mg / L is added; the germination rates of stem tips all reach 92% or above, and the rooting rates can reach 91% or above.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of kudzu root. Background technique [0002] Pueraria is a medicinal and edible plant. Its roots, stems, leaves, and flowers can be used as medicine. The stems and leaves of Pueraria can be used as excellent animal feed. Due to its tenacious adaptability and vitality, Pueraria is also a soil and water conservation plant that is highly valued by countries all over the world. , Known as "the good doctor of the earth". Modern medical research shows that isoflavones in kudzu root can treat acute myocardial infarction, unstable angina pectoris, hyperviscosity, acute cerebral infarction, cervical spondylosis and other diseases, and have antagonism effect on memory impairment caused by scopolamine and ethanol; its edible part Mainly root tubers, people extract its starch and process it into a series of foods. In recent years, the planting indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 张玉薇
Owner 张玉薇
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