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A kind of supersaturated methane saline and its preparation method and application

A technology of normal saline and methane, applied in the field of medicine, can solve the problems of increasing the burden on patients and increasing the cost of treatment, and achieve the effect of long-lasting potency and simple preparation method

Active Publication Date: 2018-10-26
FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used methods include surgery, drug therapy, gene therapy, transplantation therapy, etc., which increase the cost of treatment and the burden on patients

Method used

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  • A kind of supersaturated methane saline and its preparation method and application
  • A kind of supersaturated methane saline and its preparation method and application
  • A kind of supersaturated methane saline and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Prepare supersaturated methane saline:

[0056] Select an aluminum foil bag covered with a layer of non-toxic polyethylene film on the inner layer as a liquid storage bag and an infusion bag, which is composed of a bag body with a volume of 500ml and an infusion interface (prepared by Suzhou Xingchen New Material Co., Ltd., Jiangsu Province), and the infusion interface Sealed by a rubber seal; sterilized by γ-ray irradiation before use;

[0057] Inject sterile normal saline and methane gas (purity 99.9%) into the aluminum foil bag through the infusion port at a volume ratio of 3:1, and fill until the pressure in the aluminum foil bag reaches 0.2 MPa (from the infusion port, through the rubber seal of the infusion port) Measured by inserting a No. 6 infusion needle with a pressure gauge at one end into the ring);

[0058] Put the aluminum foil bag filled with methane into the hyperbaric chamber, raise the pressure in the hyperbaric chamber to 0.5MPa, and maintain this p...

Embodiment 2

[0062] The rat spinal cord ischemia-reperfusion injury model was injected intraperitoneally with supersaturated methane saline prepared in Example 1 immediately after reperfusion, and the injection volumes were 5ml / kg, 10ml / kg and 20ml respectively based on the weight of the rat / kg, Methane-5 group, Methane-10 group, Methane-20 group, and a blank control group, namely the IR group;

[0063] The hindlimb motor-sensory impairment index (neurobehavioral score), the number of normal neurons in the anterior horn of the lumbar spinal cord, and the methane concentration in plasma and lumbar spinal cord tissue were measured respectively after 72 hours of reperfusion. The results of the rat hindlimb motor-sensory impairment index were as follows: figure 2 As shown, the number of normal neurons in the anterior horn of the lumbar spinal cord is as follows image 3 As shown, the results of methane concentration in plasma and lumbar spinal cord tissue are as follows Figure 4 shown. ...

Embodiment 3

[0068] The rats were randomly divided into five groups, namely sham group, IR group, methane group, Si-Methane group and Con-Methane group, with 38 rats in each group. The specific information for each group is as follows:

[0069] Sham group: sham operation group, same as IR group except no spinal cord ischemia reperfusion injury.

[0070] IR group: The rat spinal cord ischemia-reperfusion injury model was prepared by blocking the thoracic aorta combined with systemic hypotension for 9 minutes and then restoring perfusion and blood pressure.

[0071] Methane group: The rat spinal cord ischemia-reperfusion injury model was prepared by blocking the thoracic aorta combined with systemic hypotension for 9 minutes and then restoring the perfusion and blood pressure; immediately after the perfusion was restored, supersaturated methane saline (MS) 20ml / kg was intraperitoneally injected .

[0072] Si-Methane group: Rats were injected intrathecally with Nrf2 small interfering RNA (N...

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Abstract

The invention relates to the field of medicines, in particular to over-saturation methane normal saline and a preparation method and application thereof. The preparation method of the over-saturation methane normal saline comprises the steps that sterile normal saline and methane gas are input an aluminum foil bag with an inner layer laminated with a polyethylene film according to the volume ratio of 3:(0.8-1.2) until the pressure in the aluminum foil bag reaches 0.18-0.22 MPa; the aluminum foil bag is treated for 6-8 hours under the pressure of 0.45-0.55 MPa; then, methane gas is input to the aluminum foil bag to supplement the part dissolved in the normal saline to enable the pressure in the aluminum foil bag to be kept at 0.18-0.22 MPa. By means of the method, an over-saturation or saturation level is achieved when the methane gas is dissolved in the normal saline, the methane gas content is high, and preparation is easy and convenient. The invention further provides application of the over-saturation methane normal saline to preparation of medicines for treating Nrf2 signal channels, especially to spinal cord ischemia reperfusion injury medicines.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a supersaturated methane saline solution and its preparation method and application. Background technique [0002] Spinal cord injury is still considered as a kind of injury without special treatment. In the past 20 years, people have carried out a lot of basic research and clinical observation on the etiology and mechanism of spinal cord injury, and realized that secondary damage after primary spinal cord injury, such as ischemia-reperfusion injury, is an important factor causing nerve damage. [0003] Spinal cord ischemia-reperfusion injury is an unpredictable complication after thoracoabdominal aortic aneurysm and spinal surgery. The incidence rate is as high as about 13-18%. The injury causes paraplegia or quadriplegia and seriously affects the quality of life of patients. However, there are still no effective control measures so far. Studies have shown that during the reperfusion p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/01A61K9/08A61K47/02A61P25/00B32B27/32B32B27/06B32B15/085B32B15/20
CPCA61K9/08A61K31/01A61K47/02B32B15/085B32B15/20B32B27/06B32B27/32
Inventor 王丽萍陈国忠
Owner FUZHOU GENERAL HOSPITAL OF NANJING MILITARY COMMAND P L A
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