Unlock instant, AI-driven research and patent intelligence for your innovation.

A new heat-resistant fungus and its application in microbial fertilizer

A microbial fertilizer and microbial agent technology, applied in the field of microbial strains and applied agriculture, filamentous fungi and their application in the preparation of microbial fertilizers, to achieve high enzyme activity, fast reproduction, and good thermal stability

Active Publication Date: 2019-09-10
新疆山川秀丽生物有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research of prior art shows that there are relatively few studies on the biological control and microbial fertilizer of silvery mildew, and silvery mildew is a kind of potential biological control of plant nematodes. Therefore, it is of practical significance to study and utilize the characteristics of this microorganism

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A new heat-resistant fungus and its application in microbial fertilizer
  • A new heat-resistant fungus and its application in microbial fertilizer
  • A new heat-resistant fungus and its application in microbial fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Isolation, screening and identification of Cunninghamella sp. TF121

[0035] 1. Separation: It was obtained from the natural composting process samples of sheep manure around sheep pens in Turpan, Xinjiang. The sample adopts the gradient dilution method, weighs 10g of the irradiated soil sample, puts it in 90ml of sterile physiological saline, activates it at 28°C-32°C for 30min-2h, then performs gradient dilution, and spreads it on a PDA medium plate for cultivation.

[0036] 2. Purification: after the growth of fungal colonies, pick out colonies of different shapes, sizes, colors, etc., and inoculate them on corresponding plates, and purify and cultivate them at 40°C-60°C, preferably at 55°C until there are no foreign colonies. Strains are preferred.

[0037] 3. Classification and identification: By observing the colony shape and physiological and biochemical characteristics, the total DNA of the strain TF121 was extracted, and the fungal ITS PCR amplifica...

Embodiment 2

[0044] Example 2: Analysis of Cunninghamella sp. TF121 CGMCC No.12624 Functional Enzyme

[0045] Using the transparent circle method, plant Cunninghamella sp. TF121 CGMCC No.12624 on the cellulase screening medium ((NH 4 ) 2 SO4 2.0g, MgSO 4 0.5g, KH 2 PO 4 1.0g, CMC-Na 2.0g, Congo red 0.4g, agar 15.0g, distilled water 1L, pH natural), amylase screening medium (soluble starch 20.0g, KCl 0.5g, NaNO 3 2.0g, KH 2 PO 4 1.0g, MgSO 4 ·7H 2 O 0.5g, agar 15.0g, distilled water 1L, pH 6.5), lipase screening medium (KH 2 PO4 0.2g, MgSO 4 0.05g, PVA emulsion 2.0mL, yeast powder 0.2g, peptone 1.0g, agar 1.7g, distilled water 1L, pH 6.5. Sterilize and cool at 50°C, add 10mL0.2% rhodamine B solution), protease screening medium (peptone 10.0g, glucose 1.0g, CaCl 2 0.1g, tyrosine 0.1g, casein 5.0g, agar 15.0g, distilled water 1L, pH 6.5, chitosan enzyme screening medium (chitosan 10.0g, (NH 4 ) 2 SO 4 2.5g, MgSO 4 ·7H 2 O 0.25g, K 2 HPO 4 0.02g, CaCO 3 6.0g, agar 20.0, pH 6.0...

Embodiment 3

[0049] Example 3: Determination of parasitism of root-knot nematode eggs by heat-resistant Cunninghamella sp. TF121 CGMCC No.12624

[0050] The isolated and purified Cunninghamella sp. TF121 CGMCC No.12624 was cultured on the slant of PDA medium for 3 days, and then 5 mL of sterile water was added to the slant test tube, shaken vigorously, and then filtered with double-layer sterile gauze. The filtrate was prepared by appropriate dilution to contain 10 6 A spore suspension was used for later use.

[0051] Select a glass petri dish with good light transmittance, add sterilized 0.1% chloramphenicol water agar (distilled water 1L, agar 10g, add chloramphenicol after sterilization to a final concentration of 0.1%), and make a thickness of about 1.5mm Flat TLC. Add 10 μL of spore suspension of Cunninghamella sp. TF121 CGMCC No.12624 dropwise under sterile conditions on the water agar plate. Root-knot nematode egg suspension from the source, the petri dish was sealed with parafil...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new species of heat-resistant fungus and its application in microbial fertilizer. A new species of heat-resistant fungus TF121 is obtained by breeding from natural compost samples of sheep manure in Turpan, Xinjiang, and is tested by preparing microbial fertilizer. Root-knot nematode eggs derived from cotton, apples, tomatoes, cucumbers and eggplants all have parasitic effects, the parasitizing rate of eggs is 22.1%-32.2%, and the average parasitizing rate is 26.6%. This effect test is applied to cotton and greenhouse tomato crops It can be seen that the present invention utilizes the screened new species of silvery mildew TF121 to significantly increase the yield of crops, and for economic crops such as greenhouse cucumbers and greenhouse eggplants, as well as fruit trees such as apples and fragrant pears, it has obvious yield and synergistic effects. At the same time, it can effectively reduce the incidence of root-knot nematodes. It can be seen that the development and application of preparations prepared by using the new species of silvery mildew xiaograms TF121 provided by the present invention has a very large application prospect.

Description

technical field [0001] The invention relates to the technical field of microbial strains and applied agriculture, in particular to the technical field of a filamentous fungus and its application in the preparation of microbial fertilizers. Background technique [0002] The genus Cunningella is a type of filamentous fungus, which can be divided into 5 species according to the morphological and physiological characteristics: C. chinensis (short thorns) C. cingae, C. bancroft, C. brucei, Brazil nut Silvery mildew and Chili (Yazhi) Silvery mildew are a family of Mucorales. They are mainly saprophytic. In addition to living in the soil, they also grow in feces, decaying plants and other substrates. [0003] Root-knot nematode (Meloidogynesp.) occurs very commonly in my country, seriously endangers crops, and is difficult to control. With the improvement of people's ecological awareness and the restriction of highly toxic chemical pesticides, the isolation and screening of high-e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/20C05G3/02C12R1/645C12R1/125C12R1/885C05G3/60
CPCC05F3/00C12N1/14C12N1/20C05G3/60C12N1/145C12R2001/645C05F11/00C05F11/02C05F11/08C05F17/00
Inventor 王晓桦张謇汪玲许咏梅杨金钰栾作涛冯新玉崔艺凡李新梅唐鹏王春燕唐天成
Owner 新疆山川秀丽生物有限公司